Anxiolytic effects of polydatin through the blockade of neuroinflammation in a chronic pain mouse model

Abstract

Background: Chronic pain is frequently comorbid with anxiety disorder, thereby complicating its treatment. Polydatin, a component from the root of Polygonum cuspidatum, has shown neuroprotection in the central nervous system. However, its effects on pain and anxiety processing have been rarely investigated. In this study, mice were injected with complete Freund's adjuvant (CFA) at the hindpaw to induce pain-and anxiety-like behaviors. Results: Treatment with polydatin (25 mg/kg) alleviated the anxiety-like behaviors but not pain perception in these mice. Polydatin treatment reversed the upregulation of N-methyl-D-aspartic acid receptors and GluA1-containing a-amino-3hydroxy-5-methyl-4-isoxazole-propionic acid receptors in the amygdala of CFA-injected mice. Additionally, this treatment reduced the levels of proinflammatory cytokines, namely, tumor necrosis factor-alpha and interleukin-1b, in the amygdala. Furthermore, activated nuclear factor kappa-B signaling was blocked in the amygdala from CFA-injected mice. By using docking technology, we found potential structural binding between polydatin and IjB kinase beta. Conclusion: This study indicates the anxiolytic effects of polydatin by suppressing inflammatory cytokines in the amygdala.

Temporomandibular inflammation mobilizes parvalbumin and FosB/deltaFosB neurons of amygdala and dorsal raphe

“…Again, the CFA-injected mouse model is used to induce pain and anxiety-like behaviors synchronously. In accordance, Guan et al (31) administered CFA in the hind paw to produce pain-and anxiety-like behaviors, and 7 days later, they observed the anxiolytic effects of polydatin by suppressing inflammatory cytokines in the amygdala in this model. Corroborating such findings, our previous data are consistent in suggesting the CFA-experimental model for temporomandibular inflammatory nociception for 10 days as generating an anxiolytic-like response (2).…”

The upshot of Polyphenolic compounds on immunity amid COVID-19 pandemic and other emerging communicable diseases: An appraisal

“…A study has demonstrated that polydatin improves lipopolysaccharide (LPS)-induced acute respiratory distress syndrome (ARDS). In addition, polydatin has mediated Parkin-dependent mitophagy and protects against mitochondria-dependent apoptosis in ARDS [ 209 , 210 ] niae (MP) infection, MP can infect both the upper and lower respiratory tracts, was found to be suppressed by polydatin treatment through inhibition of the NACHT domain-, leucine-rich repeat-, and pyd-containing protein 3 inflammasome (NLRP3) and the nuclear factor-κB pathway after MP infection [ 211 ]. Actually, several in vivo studies have reported an important protective role of polydatin treatment via different mechanisms in ovalbumin-induced bronchial asthma as well as in LPS-induced acute lung injury [ 212 , 213 ].…”

Electroacupuncture Attenuates Neuropathic Pain and Comorbid Negative Behavior: The Involvement of the Dopamine System in the Amygdala

“…Our results showing that exposure to CCI increased TNFα and IL-1β in the amygdala is consistent with these findings. One study showed that suppressing inflammatory cytokines in the amygdala could alleviate the anxiety-like behavior in a chronic pain mouse model (Guan et al, 2020). The administration of IL-1β-neutralizing antibody prevents the spared nerve injury-induced memory deficits and depressive and pain behaviors (Gui et al, 2016).…”

“…It is well known that excessive neuroinflammation is one of the key mechanisms for generating and sustaining chronic pain (Ji et al, 2014). Accumulating evidence suggests that high levels of neuroinflammation occur in the amygdala (Burke et al, 2013;Guan et al, 2020). Recently, emerging evidence has suggested that the dopaminergic system plays a critical role in regulating motivational and emotional behavior, including mood and pain modulation (Yang et al, 2020).…”