Anxiolytic effect of the heartwood of Haematoxylum campechianum L. and sappanchalcone in an in vivo model in mice

Escobar-Ramos, Armando; Gómez-Rivera, Abraham; Lobato‐García, Carlos E.; Zamilpa, Alejandro; Blé-González, Ever A.; González-Cortazar, Manasés; Gallegos-García, Ammy Joana; Herrera-Ruíz, Maribel

doi:10.1016/j.jep.2021.114764

Cited by 5 publications

(1 citation statement)

References 38 publications

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“…14,15 The molecular formula of sappanchalcone (SC) is C 16 H 14 O 5 , and its pharmacologic effects include anti-inflammatory, [16][17][18][19] antitumor, [20][21][22] bone-protective 19 and anxiolytic effects. 23 A previous study found that SC is effective in alleviating collagen-induced arthritis (CIA) in a mouse model of rheumatoid arthritis. It also showed that SC promotes wound healing and decreases the expression of inflammatory factors.…”

Section: Introductionmentioning

confidence: 99%

The osteogenic effects of sappanchalcone in vitro and in vivo

Zheng,

Chen,

Liu

et al. 2023

J of Periodontal Research

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Background and ObjectivesThe utilization of natural products to enhance the function of periodontal ligament cells (PDLCs) has emerged as a popular area of research. Recent investigations have demonstrated that sappanchalcone (SC) possesses pharmacological properties such as anti‐inflammatory and osteoprotective effects. This study aims to explore the impact of SC on the in vivo and in vitro osteogenic differentiation ability of PDLCs.MaterialsCell proliferation was quantified using the CCK‐8 assay, while gene expression levels were assessed through qRT–PCR analysis. Osteoblast differentiation capacity was evaluated by employing Alizarin red staining (ARS), alkaline phosphatase (ALP) staining and western blot (WB) analysis. A rat model of periodontitis was established utilizing the tether‐wire method. Micro‐CT imaging and hematoxylin and eosin (HE) staining were employed to evaluate alveolar bone resorption. Masson's trichrome staining was utilized to observe fiber alignment, whereas immunohistochemistry (IHC) techniques were applied for detecting osteogenic and inflammatory factors.ResultsThe results from the CCK‐8 assay indicate no observed cytotoxicity for concentrations of 1, 5, or 10 nM for SC treatment (p < .05), while qRT–PCR analysis demonstrates a significant decrease in inflammatory factors such as MMP‐1 and IL‐6 with treatment by SC (p < .05). Additionally, western blotting reveals an increase in protein expression levels of Runx2 and OPN within PDLCs treated with SC compared to control groups (p < .05), which is further supported by ARS and ALP staining indicating an increase in mineralized nodules formation along with elevated ALP content within these cells following treatment with this compound (p < .05). Finally, both HE staining as well as micro‐CT imaging suggest potential benefits associated with using this compound including slowing alveolar bone resorption while simultaneously promoting junctional epithelium proliferation.ConclusionsOur in vitro and in vivo findings suggest that SC can effectively enhance the inflammatory response of PDLCs and promote their osteogenic differentiation ability under inflammatory conditions, indicating its potential as a promising therapeutic agent for improving periodontal inflammation and bone formation.

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Section: Introductionmentioning

confidence: 99%

The osteogenic effects of sappanchalcone in vitro and in vivo

Zheng,

Chen,

Liu

et al. 2023

J of Periodontal Research

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Effect of Cinnamaldehyde Chalcone on Behavior in Adult Zebrafish (Danio rerio): In Silico Approach

Carneiro Romão,

Costa Siqueira,

Amâncio Ferreira

et al. 2024

Chemistry & Biodiversity

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The study focuses on the anxiolytic potential of chalcone (2E,4E)‐1‐(2‐hydroxyphenyl)‐5‐phenylpenta‐2,4‐dien‐1‐one (CHALCNM) in adult zebrafish. Successfully synthesized in 58% yield, CHALCNM demonstrated no toxicity after 96 h of exposure. In behavioral tests, CHALCNM (40 mg/kg) reduced locomotor activity and promoted less anxious behavior in zebrafish, confirmed by increased permanence in the light zone of the aquarium. Flumazenil reversed its anxiolytic effect, indicating interaction with GABAA receptors. Furthermore, CHALCNM (4 and 20 mg/kg) preserved zebrafish memory in inhibitory avoidance tests. Virtual screening and ADMET profile studies suggest high oral bioavailability, access to the CNS, favored by low topological polarity (TPSA ≤ 75 Å²) and low incidence of hepatotoxicity, standing out as a promising pharmacological agent against the GABAergic system. In molecular coupling, CHALCNM demonstrated superior affinity to diazepam for the GABAA receptor. These results reinforce the therapeutic potential of CHALCNM in the treatment of anxiety, highlighting its possible future clinical application.

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