Anxiolytic and thrombolytic investigation of methanol extract of Piper nigrum L. fruits and Sesamum indicum L. seeds

Emon, Nazim Uddin; Kaiser,; Kabir, M. D.Alamgir; Uddin, Mohammad Nazim; Jyoti, Mifta Ahmed; Tanjil, Saifullah; Rasel, Abu; Alam, Safaet; Islam, Mohammad Nazmul

doi:10.5455/jabet.2020.d121

Cited by 17 publications

(12 citation statements)

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“…Here for determining the pharmacokinetic properties (ADME) of three major compounds, the online tool SwissADME ( http://www.swissadme.ch/ ) was used [ 50 ]. Lipinski's rule of five (M.W not more than 500; H-bond donors ≤ 5; H-bond acceptors ≤ 10; Lipophilicity < 5 and molar refractivity ranging from 40 to 130) were considered to evaluate favorable drug-like properties of all compounds [ 51 ].…”

Section: Methodsmentioning

confidence: 99%

Biological and computational studies provide insights into Caesalphinia digyna Rottler stems

Emon

Alam

Sawon

et al. 2021

Biochemistry and Biophysics Reports

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Caesalpinia digyna (Rottl.) (Family: Fabaceae) is an essential medicinal plant for it's conventional uses against a kind of human disorders. This research aims to investigate the antidiarrheal, antibacterial and antifungal properties of the methanol extract of the stems extracts of the C. digyna (MECD). The in vivo antidiarrheal activity of the stem extracts were evaluated by using castor oil-induced diarrhea, castor oil-induced enteropooling and charcoal induced intestinal transit in mice model. Besides, in vitro antimicrobial potentiality of MECD was investigated by the disc diffusion method. In silico activity of the isolated compounds were performed by Schrödinger-Maestro (Version 11.1) software. In addition, The ADME/T analysis and PASS prediction were implemented by using pass online tools. In the antidiarrheal investigation, the MECD exhibited a notable inhibition rate in all test approaches which were statistically significant ( p < 0.05, p < 0.1, p < 0.01). MECD 400 mg/kg showed the maximum antidiarrheal potency in all the test methods. In vitro antimicrobial analysis unveiled that, MECD revealed higher potentiality against almost all pathogens and indicates dose-dependent activity against almost all the bacteria and fungi. In the case of in silico evaluation of anti-diarrheal, anti-bacterial and anti-fungal activity, all three isolated compounds met the pre-conditions of Lipinski's five rules for drug discovery. Pass predicted study also employed for all compounds. However, The chemical constituents of the C. digyna can be a potent source of anti-diarrheal, anti-bacterial and anti-fungal medicine and further modification and simulation studies are required to establish the effectiveness of bioactive compounds.

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Section: Methodsmentioning

confidence: 99%

Biological and computational studies provide insights into Caesalphinia digyna Rottler stems

Emon

Alam

Sawon

et al. 2021

Biochemistry and Biophysics Reports

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“…This widely approved experiment was conducted for evaluating anxiolytic activities in rodents [ 36 ]. The elevated plus-maze was positioned 50 cm above the ground.…”

Section: Methodsmentioning

confidence: 99%

CNS anti-depressant, anxiolytic and analgesic effects of Ganoderma applanatum (mushroom) along with ligand-receptor binding screening provide new insights: Multi-disciplinary approaches

Hossen

Islam

Hossain

et al. 2021

Biochemistry and Biophysics Reports

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This research was designed to evaluate the CNS depressant, anxiolytic, and analgesic action of aqueous and ethanol extract of Ganoderma applanatum , a valuable medicinal fungus used in multiple disorders belongs to Ganodermataceae family. Two extracts of G. applanatum were prepared using distilled water and ethanol as solvents and named AEGA and EEGA. Open field method, rotarod method, tail suspension method, and hole cross method were utilized for the CNS depressant action. In contrast, elevated plus-maze test and hole board method were utilized for the anxiolytic action. For determining the analgesic potential, acetic acid-induced writhing test, hot plate method, and tail immersion test were used. Besides, molecular docking has been implemented by using Discovery studio 2020, UCSF Chimera and PyRx autodock vina. At both doses (200 and 400 mg/kg) of AEGA and EEGA showed significant CNS depressant effect ( p < 0.05 to 0.001) against all four tests used for CNS depressant activity. Both doses of AEGA and EEGA exhibited important anxiolytic activity effect ( p < 0.05 to 0.001)against the EPM and hole board test. Both doses of AEGA and EEGA also exhibited a potential analgesic effect ( p < 0.05 to 0.001) against all three tests used for analgesic action. In addition, in the molecular docking the compounds obtained the scores of −5.2 to −12.8 kcal/mol. Ganoapplanin, sphaeropsidin D and cytosporone C showed the best binding affinity to the selected recptors. It can be concluded that AEGA and EEGA have potential CNS depressant, anxiolytic, and analgesic action, which can be used as a natural antidepressant, anxiolytic, and analgesic source.

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“…In current study, anti-thrombolytic efficacy of different fractionations of A. sativum are in range of 9 % to 20 %. Deep vein thrombosis, stroke and heart attack are among the cardiovascular conditions which alter the blood coagulation [34] and are modulated by antithrombogenic drugs [35]. Natural products have various antithrombogenic constituents that can be better therapeutic adjuncts than synthetic drugs as these have lesser side effects.…”

Section: Blood Protection Activitymentioning

confidence: 99%

Probing the Comparative Bioefficacy of Allium sativum L. Bulb through Different Solvents

Hussain¹,

Shahid²,

Zulfiqar³

et al. 2021

J. Mex. Chem. Soc.

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Abstract. Allium sativum (garlic), an aromatic bulbous plant is one of the most vital and oldest authenticated herbs which has been utilized from ancient times as a traditional medicine. A. sativum has cardioprotective, antimicrobial, anti-inflammatory, antidiabetic, antioxidant and anticancer properties due to a large range of phenolic compounds and sulfur containing compounds. The current study was conducted to assess the antioxidant chemistry, and numerous bioactivities of A. sativum bulbs extracts in different solvents. Ethanol fraction was the most active antioxidant and showed maximum total phenolic content (16.18 g GAE/100 g), total flavonoid content (95.04 g CE/100 g) and free radical scavenging activity (75.50 %). Methanol (4 %) fraction exhibited maximum antiglycation activity. Aqueous, n-hexane and ethyl acetate fractions exhibited maximal (52 %, 24 % and 38 %) inhibitions of alpha-amylase, alpha-glucosidase and acetylcholinesterase respectively. In an antimicrobial assay, ethanol (59.05 %) and chloroform (72.92 %) fractions showed maximum inhibition of Pasturella multocida and Staphylococcus aureus strains respectively. n-butanol and n-hexane fractions showed maximum (31 %) antihemolytic activity and (20 %) thrombolytic activities respectively. A. sativum bulb extracts and fractions have noteworthy bio-efficacies that holds promise to be used as a source of natural drug to cure various disorders. Resumen. Allium sativum (ajo), una planta bulbosa aromática, es una de las hierbas autenticadas más vitales y antiguas que se han utilizado desde la antigüedad como medicina tradicional. A. sativum tiene propiedades cardioprotectoras, antimicrobianas, anti-inflamatorias, anti-diabéticas, anti-oxidantes y anti-cancerígenas debido a una amplia gama de compuestos fenólicos y compuestos que contienen azufre. El estudio actual se llevó a cabo para evaluar la química antioxidante y numerosas bioactividades de extractos de rizoma de A. sativum en diferentes disolventes. La fracción de etanol tuvo la mayor actividad antioxidante y mostró un contenido fenólico total máximo (16.18 g GAE / 100 g), contenido total de flavonoides (95.04 g CE / 100 g) y actividad captadora de radicales libres (75.50 %). La fracción de metanol (4 %) exhibió la mayor actividad de antiglicación. Las fracciones acuosa, de n-hexano y de acetato de etilo inhibieron la actividad de alfa-amilasa, alfa-glucosidasa y acetilcolinesterasa, en 52 %, 24 % y 38 % respectivamente. En el ensayo antimicrobiano, las fracciones de etanol (59.05 %) y cloroformo (72.92 %) mostraron una inhibición máxima de las cepas de Pasturella multocida y Staphylococcus aureus, respectivamente. Las fracciones de n-butanol y n-hexano mostraron actividad anti-hemolítica (31 %) y trombolítica (20 %) respectivamente. Los extractos y fracciones de rizoma de A. sativum tienen bioeficacia notable con potencial de ser utilizadas como una fuente de fármaco natural para curar diversos trastornos.

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Anxiolytic and thrombolytic investigation of methanol extract of Piper nigrum L. fruits and Sesamum indicum L. seeds

Cited by 17 publications

References 0 publications

Biological and computational studies provide insights into Caesalphinia digyna Rottler stems

Biological and computational studies provide insights into Caesalphinia digyna Rottler stems

CNS anti-depressant, anxiolytic and analgesic effects of Ganoderma applanatum (mushroom) along with ligand-receptor binding screening provide new insights: Multi-disciplinary approaches

Probing the Comparative Bioefficacy of Allium sativum L. Bulb through Different Solvents

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