Antixenosis, tolerance and genetic analysis of some rice landraces for resistance to Nilaparvata lugens (Stål.)

Roy, Debashis; Chakraborty, Gautam; Biswas, Abhisek; Sarkar, P. K.

doi:10.1016/j.aspen.2020.10.012

Cited by 4 publications

(3 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several prior studies have shown that the antixenosis mechanism involved when feeding on incompatible or resistant hosts impairs several aspects of the developmental and physiological processes of pests [ 42 , 43 ]. A crucial structural gene whose expression showed early and sustained induction in both insects feeding on resistant rice lines is the cuticle protein.…”

Section: Discussionmentioning

confidence: 99%

Expression Analysis Reveals Differentially Expressed Genes in BPH and WBPH Associated with Resistance in Rice RILs Derived from a Cross between RP2068 and TN1

Anand,

Divya,

Mazumdar-Leighton

et al. 2023

IJMS

View full text Add to dashboard Cite

BPH (brown planthopper) and WBPH (white backed planthopper) are significant rice pests that often co-occur as sympatric species and cause substantial yield loss. Despite their genetic similarities, different host-resistance genes confer resistance against these two hoppers. The defense mechanisms in rice against these pests are complex, and the molecular processes regulating their responses remain largely unknown. This study used specific recombinant inbred lines (RILs) derived from a cross between rice varieties RP2068-18-3-5 (BPH- and WBPH-resistant) and TN1 (BPH- and WBPH-susceptible) to investigate the mechanisms of interaction between these planthoppers and their rice hosts. WBPH and BPH were allowed to feed on specific RILs, and RNA-Seq was carried out on WBPH insects. Transcriptome profiling and qRT-PCR results revealed differential expression of genes involved in detoxification, digestion, transportation, cuticle formation, splicing, and RNA processing. A higher expression of sugar transporters was observed in both hoppers feeding on rice with resistance against either hopper. This is the first comparative analysis of gene expressions in these insects fed on genetically similar hosts but with differential resistance to BPH and WBPH. These results complement our earlier findings on the differential gene expression of the same RILs (BPH- or WBPH-infested) utilized in this study. Moreover, identifying insect genes and pathways responsible for countering host defense would augment our understanding of BPH and WBPH interaction with their rice hosts and enable us to develop lasting strategies to control these significant pests.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression Analysis Reveals Differentially Expressed Genes in BPH and WBPH Associated with Resistance in Rice RILs Derived from a Cross between RP2068 and TN1

Anand,

Divya,

Mazumdar-Leighton

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Evaluation on the honeydew excretion of BPH was conducted with 30 day-old potted seedlings by following Sogawa & Pathak (1970) , as described in our previous study ( Roy et al, 2021 ). Five numbers of each 1-day-old adult females and 2 nd instar nymphs were placed separately to the lower stem portion of the 30 day-old potted seedling with an orange coloured bromocresol green treated filter paper around the base and an inverted and basal perforated transparent plastic cup (80 ml volume) on the filter paper incarcerating the insects to the stem portion of about 9 cm long.…”

Section: Methodsmentioning

confidence: 99%

“…This experiment was carried out by taking 40 selected rice landraces seeded at random rows (10 seeds per row), 3.0 cm apart in a seedbox by following the standard procedure given by Sarao & Bentur (2016) as described in our previous study ( Roy et al, 2021 ). The Swarna seeds were sown in two border rows, whereas a single row of Ptb33 was introduced in the middle of the box.…”

Section: Methodsmentioning

confidence: 99%

Evaluation and characterization of indigenous rice (Oryza sativa L.) landraces resistant to brown planthopper Nilaparvata lugens (Stål.) biotype 4

Roy

Biswas

Sarkar

et al. 2022

PeerJ

Self Cite

View full text Add to dashboard Cite

Evaluation and identification of resistant donors for brown planthopper (BPH) Nilaparvata lugens (Stål.), an economically important insect pest of rice, is a continuous process to develop new resistant rice varieties. However, several rice landraces of north-eastern India are not yet characterized for BPH resistance. In the present study, a set of 218 rice landraces were screened in both greenhouse and open-field conditions for three consecutive years, and thereafter forty selected promising entries were explored to evaluate their phenotypic and genotypic reactions against BPH biotype 4. Based on phenotypic evaluations, five landraces were identified as resistant, while 31 were moderately resistant, and grouped under the major cluster I and II, respectively, in a circular dendrogram. Antixenosis and antibiosis studies of these landraces divulged that, compared to the susceptible check variety, resistant landraces exhibited the lowest feeding rate, survival, and nymphal and adult settling, but higher frequency of unhatched eggs of BPH. Un-infested resistant landraces registered higher levels of ascorbic acid, oxalic acid and crude silica, however, elevated levels of total free amino acid, potassium and crude silica were observed under BPH herbivory. The present study focuses on identifying new donors having BPH resistance resources which could be useful in genomic studies for the development of BPH biotype 4 resistant rice varieties.

show abstract

Evaluating, Screening and Selecting Yardlong Bean [Vigna unguiculata subsp. sesquipedalis (L.) Verdc.] for Resistance to Common Cutworm (Spodoptera litura Fabricius)

Yao

Jiang

et al. 2023

Agronomy

View full text Add to dashboard Cite

The yardlong bean [Vigna unguiculata subsp. sesquipedalis (L.) Verdc.] is an important vegetable crop, but it is prone to pest infestation. Therefore, breeding insect-resistant varieties is essential to reduce pesticide applications and to increase bean quality and yield. In the present study, 64 yardlong bean varieties were screened for their resistance to the common cutworm (Spodoptera litura Fabricius). In the greenhouse, leaves, pods, and seeds of yardlong beans were harmed by naturally occurring common cutworms. Seventeen insect-resistant and four insect-susceptible yardlong bean varieties were identified based on the weight of the nine-day-old larvae and 72 h weight increases of 4th instar larvae through feeding newly hatched and 4th instar larvae, respectively. Subsequent verification feeding experiments with newly hatched larvae showed that Zhuzaidou and Pingtangjiangdou’s insect resistance are the weakest and Jiangdou No.5, j-1, Zhijiangtezao No. 30, and Changcaidou have the strongest insect resistance. In 21 yardlong bean varieties, starch content and larval weight showed negative correlation and there was positive correlation between crude protein and larval weight, but almost neither of them reached significant levels. Through organ antibiotic and antixenotic experiments, it was concluded that common cutworms preferred feeding on yardlong bean leaves, and the weight increase of common cutworms feeding on leaves was higher than that of pods and seeds. These insect-resistant yardlong bean varieties warrant further investigation in basic antibiosis mechanism research in yardlong beans and can serve as germplasm resources for breeding programs engaged in reducing pesticide usage.

show abstract

Antixenosis, tolerance and genetic analysis of some rice landraces for resistance to Nilaparvata lugens (Stål.)

Cited by 4 publications

References 38 publications

Expression Analysis Reveals Differentially Expressed Genes in BPH and WBPH Associated with Resistance in Rice RILs Derived from a Cross between RP2068 and TN1

Expression Analysis Reveals Differentially Expressed Genes in BPH and WBPH Associated with Resistance in Rice RILs Derived from a Cross between RP2068 and TN1

Evaluation and characterization of indigenous rice (Oryza sativa L.) landraces resistant to brown planthopper Nilaparvata lugens (Stål.) biotype 4

Evaluating, Screening and Selecting Yardlong Bean [Vigna unguiculata subsp. sesquipedalis (L.) Verdc.] for Resistance to Common Cutworm (Spodoptera litura Fabricius)

Contact Info

Product

Resources

About