Antiviral properties of structural fragments of the peptide Selank

Andreeva, L. A.; Nagaev, I. Yu.; Mezentseva, M. V.; Shapoval, I. M.; Podchernyaeva, R. Ya.; Щербенко, В. Э.; Potapova, Lyudmila Aleksandrovna; Russu, L. I.; Ершов, Ф. И.; Myasoedov, N. F.

doi:10.1134/s0012496610020031

Cited by 7 publications

(4 citation statements)

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“…Previously, it was shown that Selank causes a marked change in the expression of genes involved in inflammatory processes in the hippocampus and spleen of rodents (Kolomin et al, 2010 , 2011 , 2014 ). Our results have confirmed at the molecular level that the clinical effects observed after the introduction of Selank are related to its antiviral activity (Ershov et al, 2009 ; Andreeva et al, 2010 ).…”

Section: Introductionsupporting

confidence: 84%

“…Synthetic analogs of regulatory peptides typically contain only natural amino acids in their structure, so that they practically do not have any toxic side effects. Drugs that are developed on the basis of regulatory peptides can provide directional effects on certain human body systems and are already used for the treatment of a variety of human diseases, such as cardiovascular disease (Gusev et al, 1997 ), gastrointestinal disease (Ivanov Iu and Iasnetsov, 2000 ), viral infections (Ershov et al, 2009 ; Andreeva et al, 2010 ), and various pathologies of the nervous system (Gusev et al, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

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GABA, Selank, and Olanzapine Affect the Expression of Genes Involved in GABAergic Neurotransmission in IMR-32 Cells

et al. 2017

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Clinical studies have shown that Selank had an anxiolytic effect comparable to that of classical benzodiazepine drugs, which can enhance the inhibitory effect of GABA by allosteric modulation of GABAA receptors. These data suggest that the molecular mechanism of the effect of Selank may also be related to its ability to affect the performance of the GABAergic system. To test this hypothesis, we studied the changes in expression of 84 genes involved in the functioning of the GABAergic system and in the processes of neurotransmission in the culture of neuroblastoma IMR-32 cells using qPCR method. As test substances, in addition to Selank, we selected the major GABAA receptor ligand, GABA, the atypical antipsychotic, olanzapine, and combinations of these compounds (Selank and GABA; Selank and olanzapine). We found no changes in the mRNA levels of the genes studied under the effect of Selank. The combined effect of GABA and Selank led to nearly complete suppression of changes in expression of genes in which mRNA levels changed under the effect of GABA. When Selank was used in conjunction with olanzapine, the expression alterations of more genes were observed compared with olanzapine alone. The data obtained indicate that Selank has no direct effect on the mRNA levels of the GABAergic system genes in neuroblastoma IMR-32 cells. At the same time, our results partially confirm the hypothesis that the peptide may affect the interaction of GABA with GABAA receptors. Our data also suggest that Selank may enhance the effect of olanzapine on the expression of the genes studied.

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Section: Introductionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

GABA, Selank, and Olanzapine Affect the Expression of Genes Involved in GABAergic Neurotransmission in IMR-32 Cells

et al. 2017

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“…The study of the antiviral properties of the structural fragments of the Selank peptide allowed the selection of Gly-Pro as the minimum amino acid sequence (pharmacophore) that had a pronounced antiviral effect. Among the fragments of Selank studied, the tetrapeptide Arg-Pro-Gly-Pro exhibited the highest antiviral activity against the human influenza A/Aichi2/68 virus (H 3 N 2 ), the human influenza B/Ohio01/05 virus, the avian influenza virus (H 5 N 1 ), the herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), the cytomegalovirus (CMV), and the murine encephalomyocarditis virus (EMCV) [182].…”

Section: Immunomodulatory Effectmentioning

confidence: 99%

“…Three Selank fragments were selected for analysis: the natural regulatory peptide tuftsin, with a macrophageactivating effect, the Arg-Pro-Gly-Pro peptide, which exhibited the largest antiviral activity, and the dipeptide Gly-Pro, which is the smallest structural Selank unit that retains antiviral activity [182]. Selank and its fragments caused changes in the expression of 35 genes involved in inflammation (chemokines, cytokines, and their receptors) in the mouse spleen at 6 h and 24 h after a single intraperitoneal injection of the drug.…”

Section: Effect Of Selank On the Transcriptomementioning

confidence: 99%

A New Generation of Drugs: Synthetic Peptides Based on Natural Regulatory Peptides

Kolomin¹,

Шадрина²,

Slominsky³

et al. 2013

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Natural regulatory peptides are biologically active compounds that are produced by various cells and provide a link among the main regulatory systems of the body. The field of research into the biologic activity of endogenous regulatory peptides is extremely vast. These peptides affect the cardiovascular, immune, reproductive, endocrine, digestive, and other systems, alter energy metabolism, and are especially effective in the regulation of the central nervous system. Despite of the wide range of preventive and therapeutic effects of natural regulatory peptides and proteins, their application in clinical practice is difficult. This is primarily because of their extreme instability, as they are rapidly degraded by proteases of the gastrointestinal tract, blood, cerebrospinal fluid, and other biologic media. Compounds with higher stability (i.e., a considerably longer half-life compared with that of natural molecules) and the ability to provide a directional effect on the various body systems were obtained from modifications of endogenous regulatory peptides. Synthetic analogs of regulatory peptides, as a rule, contain only natural amino acids in their composition, and their biodegradation does not lead to the formation of toxic products; thus, they have fewer side effects. This review focuses on the consideration of two synthetic regulatory peptides, Semax and Selank, which were the bases for the creation of new drugs that are used effectively in the treatment of various diseases of the nervous system. The synthetic analog of an adrenocorticotropic hormone 4-10 fragment (ACTH 4-10) Semax is a powerful neuroprotective agent that is particularly effective as a therapy for stroke. Selank was synthesized on the basis of the natural immunomodulator tuftsin. Selank is a powerful anxiolytic that is used as a therapy for generalized anxiety disorder and neurasthenia without sedative and muscle-relaxant effects. This review presents the results of research aimed at studying the influence of these peptides on the transcriptome of brain cells. The problems of drugs developed based on the clinical activities of Semax and Selank are discussed separately.

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The temporary dynamics of inflammation-related genes expression under tuftsin analog Selank action

Kolomin

Morozova

Волкова

et al. 2014

Molecular Immunology

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Antiviral properties of structural fragments of the peptide Selank

Cited by 7 publications

References 1 publication

GABA, Selank, and Olanzapine Affect the Expression of Genes Involved in GABAergic Neurotransmission in IMR-32 Cells

GABA, Selank, and Olanzapine Affect the Expression of Genes Involved in GABAergic Neurotransmission in IMR-32 Cells

A New Generation of Drugs: Synthetic Peptides Based on Natural Regulatory Peptides

The temporary dynamics of inflammation-related genes expression under tuftsin analog Selank action

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