Antiviral Immunity in the Red Swamp Crayfish , Procambarus Clarkii : Hemocyte Production , Proliferation and Apoptosis

El-Din, Abdel-Hakim S.; Varjabedian, Kohar Garo; Abdel‐Gaber, Rewaida; Mohamed, Marwa M.

doi:10.12816/0006368

Cited by 5 publications

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“…P. clarkii is a highly adaptable, tolerant, and fecund freshwater crayfish that can inhabit a wide range of aquatic environments, including those with moderate salinity, low oxygen levels, extreme temperatures, and pollution [2] , [5] . Because of these characteristics, in addition to its economic role, the crayfish has become an important crustacean model organism in research on viral infection [6] – [10] , animal behavior [11] – [17] , and environmental stress and toxicity [18] – [23] .…”

Section: Introductionmentioning

confidence: 99%

In-Depth Transcriptome Analysis of the Red Swamp Crayfish Procambarus clarkii

Shen

et al. 2014

PLoS ONE

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The red swamp crayfish Procambarus clarkii is a highly adaptable, tolerant, and fecund freshwater crayfish that inhabits a wide range of aquatic environments. It is an important crustacean model organism that is used in many research fields, including animal behavior, environmental stress and toxicity, and studies of viral infection. Despite its widespread use, knowledge of the crayfish genome is very limited and insufficient for meaningful research. This is the use of next-generation sequencing techniques to analyze the crayfish transcriptome. A total of 324.97 million raw reads of 100 base pairs were generated, and a total of 88,463 transcripts were assembled de novo using Trinity software, producing 55,278 non-redundant transcripts. Comparison of digital gene expression between four different tissues revealed differentially expressed genes, in which more overexpressed genes were found in the hepatopancreas than in other tissues, and more underexpressed genes were found in the testis and the ovary than in other tissues. Gene ontology (GO) and KEGG enrichment analysis of differentially expressed genes revealed that metabolite- and immune-related pathway genes were enriched in the hepatopancreas, and DNA replication-related pathway genes were enriched in the ovary and the testis, which is consistent with the important role of the hepatopancreas in metabolism, immunity, and the stress response, and with that of the ovary and the testis in reproduction. It was also found that 14 vitellogenin transcripts were highly expressed specifically in the hepatopancreas, and 6 transcripts were highly expressed specifically in the ovary, but no vitellogenin transcripts were highly expressed in both the hepatopancreas and the ovary. These results provide new insight into the role of vitellogenin in crustaceans. In addition, 243,764 SNP sites and 43,205 microsatellite sequences were identified in the sequencing data. We believe that our results provide an important genome resource for the crayfish.

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Section: Introductionmentioning

confidence: 99%

In-Depth Transcriptome Analysis of the Red Swamp Crayfish Procambarus clarkii

Shen

et al. 2014

PLoS ONE

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A mitochondrial outer membrane-localized protein encoded by White spot syndrome virus

Yan

Liang

et al. 2016

Virus Genes

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White spot syndrome virus (WSSV) is a devastating pathogen of crustaceans that causes huge losses to global shrimp farming. In this study, a mitochondrial outer membrane protein encoded by WSSV, termed as wsv152, was identified. Bioinformatics analysis showed that wsv152 shared no similarity with any known proteins. Real-time RT-PCR revealed that wsv152 was initially transcribed at 6 h post WSSV infection. Fluorescent microscopy demonstrated that the wsv152-GFP fusion protein was present in the mitochondrion. Western blot further suggested that wsv152 was specifically localized on the outer membrane of mitochondria. To our knowledge, this is the first report of a mitochondrion-localized protein encoded by WSSV.

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WSV152 induces apoptosis and promotes viral replication in Litopenaeus vannamei

Yan

Liu

et al. 2020

Fish & Shellfish Immunology

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Antiviral Immunity in the Red Swamp Crayfish , Procambarus Clarkii : Hemocyte Production , Proliferation and Apoptosis

Cited by 5 publications

References 0 publications

In-Depth Transcriptome Analysis of the Red Swamp Crayfish Procambarus clarkii

In-Depth Transcriptome Analysis of the Red Swamp Crayfish Procambarus clarkii

A mitochondrial outer membrane-localized protein encoded by White spot syndrome virus

WSV152 induces apoptosis and promotes viral replication in Litopenaeus vannamei

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