Antiviral effects of Psidium guajava Linn. (guava) tea on the growth of clinical isolated H1N1 viruses: Its role in viral hemagglutination and neuraminidase inhibition

Sriwilaijaroen, Nongluk; Fukumoto, Syuichi; Kumagai, Kazuyoshi; Hiramatsu, Hiroaki; Odagiri, Takato; Tashiro, Masato; Suzuki, Yasuo

doi:10.1016/j.antiviral.2012.02.013

Cited by 68 publications

(34 citation statements)

References 29 publications

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“…Inhibition of influenza virus propagation was measured using AX4 cells in the presence of serial dilutions of 2a,3ax-difluoroNeu5Ac (0.1-100 mM) 37,51 . Influenza virus at a multiplicity of infection of 0.03 was preincubated with a medium containing 2 mg ml À 1 acetylated trypsin in the absence or presence of serially diluted inhibitor for 1 h at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Influenza neuraminidase operates via a nucleophilic mechanism and can be targeted by covalent inhibitors

et al. 2013

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Section: Methodsmentioning

confidence: 99%

Influenza neuraminidase operates via a nucleophilic mechanism and can be targeted by covalent inhibitors

et al. 2013

Self Cite

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“…Furthermore, guava leaf tea helped control of the growth of influenza viruses, including oseltamivir-resistant strains, via the prevention of viral entry into host cells, probably due to the presence of flavonols [36]. …”

Section: Pharmacological Propertiesmentioning

confidence: 99%

Health Effects of Psidium guajava L. Leaves: An Overview of the Last Decade

Díaz‐de‐Cerio

Verardo

Gómez-Caravaca

et al. 2017

IJMS

123

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Today, there is increasing interest in discovering new bioactive compounds derived from ethnomedicine. Preparations of guava (Psidium guajava L.) leaves have traditionally been used to manage several diseases. The pharmacological research in vitro as well as in vivo has been widely used to demonstrate the potential of the extracts from the leaves for the co-treatment of different ailments with high prevalence worldwide, upholding the traditional medicine in cases such as diabetes mellitus, cardiovascular diseases, cancer, and parasitic infections. Moreover, the biological activity has been attributed to the bioactive composition of the leaves, to some specific phytochemical subclasses, or even to individual compounds. Phenolic compounds in guava leaves have been credited with regulating blood-glucose levels. Thus, the aim of the present review was to compile results from in vitro and in vivo studies carried out with guava leaves over the last decade, relating the effects to their clinical applications in order to focus further research for finding individual bioactive compounds. Some food applications (guava tea and supplementary feed for aquaculture) and some clinical, in vitro, and in vivo outcomes are also included.

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“…The search for plant-based antivirals against the influenza virus is promising, as several plants have been shown to possess anti-influenza activity, some of which include: Thuja orientalis , Aster spathulifolius , Pinus thunbergia [16], Allium fistulosum [17], Sambucus nigra [18] and Psidium guajava [19]. Active components have also been isolated from crude plant extracts employing chemical fractionation techniques.…”

Section: Introductionmentioning

confidence: 99%

Identification of Traditional Medicinal Plant Extracts with Novel Anti-Influenza Activity

et al. 2013

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The emergence of drug resistant variants of the influenza virus has led to a need to identify novel and effective antiviral agents. As an alternative to synthetic drugs, the consolidation of empirical knowledge with ethnopharmacological evidence of medicinal plants offers a novel platform for the development of antiviral drugs. The aim of this study was to identify plant extracts with proven activity against the influenza virus. Extracts of fifty medicinal plants, originating from the tropical rainforests of Borneo used as herbal medicines by traditional healers to treat flu-like symptoms, were tested against the H1N1 and H3N1 subtypes of the virus. In the initial phase, in vitro micro-inhibition assays along with cytotoxicity screening were performed on MDCK cells. Most plant extracts were found to be minimally cytotoxic, indicating that the compounds linked to an ethnomedical framework were relatively innocuous, and eleven crude extracts exhibited viral inhibition against both the strains. All extracts inhibited the enzymatic activity of viral neuraminidase and four extracts were also shown to act through the hemagglutination inhibition (HI) pathway. Moreover, the samples that acted through both HI and neuraminidase inhibition (NI) evidenced more than 90% reduction in virus adsorption and penetration, thereby indicating potent action in the early stages of viral replication. Concurrent studies involving Receptor Destroying Enzyme treatments of HI extracts indicated the presence of sialic acid-like component(s) that could be responsible for hemagglutination inhibition. The manifestation of both modes of viral inhibition in a single extract suggests that there may be a synergistic effect implicating more than one active component. Overall, our results provide substantive support for the use of Borneo traditional plants as promising sources of novel anti-influenza drug candidates. Furthermore, the pathways involving inhibition of hemagglutination could be a solution to the global occurrence of viral strains resistant to neuraminidase drugs.

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Antiviral effects of Psidium guajava Linn. (guava) tea on the growth of clinical isolated H1N1 viruses: Its role in viral hemagglutination and neuraminidase inhibition

Cited by 68 publications

References 29 publications

Influenza neuraminidase operates via a nucleophilic mechanism and can be targeted by covalent inhibitors

Influenza neuraminidase operates via a nucleophilic mechanism and can be targeted by covalent inhibitors

Health Effects of Psidium guajava L. Leaves: An Overview of the Last Decade

Identification of Traditional Medicinal Plant Extracts with Novel Anti-Influenza Activity

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