Antiviral effects of Brazilian green and red propolis extracts on Enterovirus surrogates

Silva-Beltrán, Norma Patricia; Balderrama-Carmona, Ana Paola; Umsza-Guez, Marcelo Andrés; Machado, Bruna Aparecida Souza

doi:10.1007/s11356-019-07458-z

Cited by 28 publications

(19 citation statements)

References 48 publications

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“…As mentioned, antiviral efficacy depends on the extraction method. A study comparing the antiviral properties of green and red Brazilian propolis extract prepared using two different ultrasonic methods and maceration found that both ultrasonic extracts demonstrated greater activity against bacteriophages MS2 and Av-08 than the maceration extract, while the maceration extract of red propolis was more active than the corresponding green extract for damaging the viral cell membrane and inhibiting polymerase activity [50]. Recently, a Euro-Asian poplar propolis extract treatment for 4 days was found to inhibit H1N1 influenza virus infection of MDCK cells by suppressing both viral growth and neuraminidase (NA) activity (Table 1) [31].…”

Section: Anti-viral Activitymentioning

confidence: 99%

Anti-Viral and Immunomodulatory Properties of Propolis: Chemical Diversity, Pharmacological Properties, Preclinical and Clinical Applications, and In Silico Potential against SARS-CoV-2

Yosri

El-Wahed

Ghonaim

et al. 2021

Foods

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Propolis, a resin produced by honeybees, has long been used as a dietary supplement and folk remedy, and more recent preclinical investigations have demonstrated a large spectrum of potential therapeutic bioactivities, including antioxidant, antibacterial, anti-inflammatory, neuroprotective, immunomodulatory, anticancer, and antiviral properties. As an antiviral agent, propolis and various constituents have shown promising preclinical efficacy against adenoviruses, influenza viruses, respiratory tract viruses, herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), human immunodeficiency virus (HIV), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Over 300 chemical components have been identified in propolis, including terpenes, flavonoids, and phenolic acids, with the specific constituent profile varying widely according to geographic origin and regional flora. Propolis and its constituents have demonstrated potential efficacy against SARS-CoV-2 by modulating multiple pathogenic and antiviral pathways. Molecular docking studies have demonstrated high binding affinities of propolis derivatives to multiple SARS-CoV-2 proteins, including 3C-like protease (3CLpro), papain-like protease (PLpro), RNA-dependent RNA polymerase (RdRp), the receptor-binding domain (RBD) of the spike protein (S-protein), and helicase (NSP13), as well as to the viral target angiotensin-converting enzyme 2 (ACE2). Among these compounds, retusapurpurin A has shown high affinity to 3CLpro (ΔG = −9.4 kcal/mol), RdRp (−7.5), RBD (−7.2), NSP13 (−9.4), and ACE2 (−10.4) and potent inhibition of viral entry by forming hydrogen bonds with amino acid residues within viral and human target proteins. In addition, propolis-derived baccharin demonstrated even higher binding affinity towards PLpro (−8.2 kcal/mol). Measures of drug-likeness parameters, including metabolism, distribution, absorption, excretion, and toxicity (ADMET) characteristics, also support the potential of propolis as an effective agent to combat COVID-19.

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Section: Anti-viral Activitymentioning

confidence: 99%

Anti-Viral and Immunomodulatory Properties of Propolis: Chemical Diversity, Pharmacological Properties, Preclinical and Clinical Applications, and In Silico Potential against SARS-CoV-2

Yosri

El-Wahed

Ghonaim

et al. 2021

Foods

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“…[ 71 ] Several substances have been already described for their antiviral action, including propolis. [ 72 ]…”

Section: Propolis: Safety/side Effects and Immunomodulatory Actionmentioning

confidence: 99%

“…Cytotoxic assays included cell count, reduction markers and the analysis of morphological changes. More recently, Silva-Beltrán et al [ 72 ] employed MS2 and AV-08 bacteriophages to assess propolis activity against enterovirus. These bacteriophages have genetic and structural characteristics similar to enteric viruses and are considered a good model to evaluate new anti-enterovirus drugs in vitro .…”

Section: Evidence For Propolis Antiviral Actionmentioning

confidence: 99%

Propolis antiviral and immunomodulatory activity: a review and perspectives for COVID-19 treatment

Ripari

Sartori

Honorio

et al. 2021

Journal of Pharmacy and Pharmacology

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Objectives Viral outbreaks are a frequent concern for humans. A great variety of drugs has been used to treat viral diseases, which are not always safe and effective and may induce adverse effects, indicating the need for new antiviral drugs extracted from natural sources. Propolis is a bee-made product exhibiting many biological properties. An overview of viruses, antiviral immunity, propolis safety and its immunomodulatory and antiviral action is reported, as well as perspectives for coronavirus disease 2019 (COVID-19) treatment. PubMed platform was used for data collection, searching for the keywords “propolis”, “virus”, “antiviral”, “antimicrobial” and “coronavirus”. Key findings Propolis is safe and exerts antiviral and immunomodulatory activity; however, clinical trials should investigate its effects on individuals with viral diseases, in combination or not with antiviral drugs or vaccines. Summary Regarding COVID-19, the effects of propolis should be investigated directly on the virus in vitro or on infected individuals alone or in combination with antiviral drugs, due to its immunomodulatory and anti-inflammatory action. Propolis administration simultaneously with vaccines should be analyzed, due to its adjuvant properties, to enhance the individuals’ immune response. The search for therapeutic targets may be useful to find out how propolis can help to control COVID-19.

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“…Brazil is the major producer of propolis and this natural substance can be classified according to its color. Green propolis induced a 3.0 log 10 reduction in the infectivity of MS2 and 3.5 log10 in Av-08; red propolis was even more effective in reducing PFU, with a 4.2 and 4.0 log10 reduction for MS2 and Av-08 [ 96 ]. The main active molecule of red propolis is formononetin and the suggested mechanism of inhibition was alteration of the structure of the capsid [ 131 ].…”

Section: Effect Of Dietary Compounds On Phagesmentioning

confidence: 99%

Dietary Modulation of Bacteriophages as an Additional Player in Inflammation and Cancer

Marongiu

Burkard

Venturelli

et al. 2021

Cancers

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Natural compounds such as essential oils and tea have been used successfully in naturopathy and folk medicine for hundreds of years. Current research is unveiling the molecular role of their antibacterial, anti-inflammatory, and anticancer properties. Nevertheless, the effect of these compounds on bacteriophages is still poorly understood. The application of bacteriophages against bacteria has gained a particular interest in recent years due to, e.g., the constant rise of antimicrobial resistance to antibiotics, or an increasing awareness of different types of microbiota and their potential contribution to gastrointestinal diseases, including inflammatory and malignant conditions. Thus, a better knowledge of how dietary products can affect bacteriophages and, in turn, the whole gut microbiome can help maintain healthy homeostasis, reducing the risk of developing diseases such as diverse types of gastroenteritis, inflammatory bowel disease, or even cancer. The present review summarizes the effect of dietary compounds on the physiology of bacteriophages. In a majority of works, the substance class of polyphenols showed a particular activity against bacteriophages, and the primary mechanism of action involved structural damage of the capsid, inhibiting bacteriophage activity and infectivity. Some further dietary compounds such as caffeine, salt or oregano have been shown to induce or suppress prophages, whereas others, such as the natural sweeter stevia, promoted species-specific phage responses. A better understanding of how dietary compounds could selectively, and specifically, modulate the activity of individual phages opens the possibility to reorganize the microbial network as an additional strategy to support in the combat, or in prevention, of gastrointestinal diseases, including inflammation and cancer.

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Antiviral effects of Brazilian green and red propolis extracts on Enterovirus surrogates

Cited by 28 publications

References 48 publications

Anti-Viral and Immunomodulatory Properties of Propolis: Chemical Diversity, Pharmacological Properties, Preclinical and Clinical Applications, and In Silico Potential against SARS-CoV-2

Anti-Viral and Immunomodulatory Properties of Propolis: Chemical Diversity, Pharmacological Properties, Preclinical and Clinical Applications, and In Silico Potential against SARS-CoV-2

Propolis antiviral and immunomodulatory activity: a review and perspectives for COVID-19 treatment

Dietary Modulation of Bacteriophages as an Additional Player in Inflammation and Cancer

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