Antiviral Effect of Interferon Lambda Against Lymphocytic Choriomeningitis Virus

Lukáčiková, Ľubomíra; Ovečková, Ingrid; Betáková, Tatiana; Laposova, Katarina; Polcicova, Katarina; Pastoreková, Silvia; Pastorek, Jaromı́r; Tomaskova, Jana

doi:10.1089/jir.2014.0083

Cited by 12 publications

(4 citation statements)

References 75 publications

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“…We hypothesize that these variations in IFN-induced ISGs contribute to differential levels of NO induction. One such limitation of our study is that we did not verify the expression of IFN receptors on the lung epithelial cells, although these cells are known to respond to the different IFNs (18,77) and express the distinct IFN receptors (75, 78,79).…”

Section: Discussionmentioning

confidence: 99%

IFN-γ-mediated control of SARS-CoV-2 infection through nitric oxide

Silva,

Krogstad,

Teles

et al. 2023

Front. Immunol.

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IntroductionThe COVID-19 pandemic has highlighted the need to identify mechanisms of antiviral host defense against SARS-CoV-2. One such mediator is interferon-g (IFN-γ), which, when administered to infected patients, is reported to result in viral clearance and resolution of pulmonary symptoms. IFN-γ treatment of a human lung epithelial cell line triggered an antiviral activity against SARS-CoV-2, yet the mechanism for this antiviral response was not identified.MethodsGiven that IFN-γ has been shown to trigger antiviral activity via the generation of nitric oxide (NO), we investigated whether IFN-γ induction of antiviral activity against SARS-CoV-2 infection is dependent upon the generation of NO in human pulmonary epithelial cells. We treated the simian epithelial cell line Vero E6 and human pulmonary epithelial cell lines, including A549-ACE2, and Calu-3, with IFN-γ and observed the resulting induction of NO and its effects on SARS-CoV-2 replication. Pharmacological inhibition of inducible nitric oxide synthase (iNOS) was employed to assess the dependency on NO production. Additionally, the study examined the effect of interleukin-1b (IL-1β) on the IFN-g-induced NO production and its antiviral efficacy.ResultsTreatment of Vero E6 cells with IFN-γ resulted in a dose-responsive induction of NO and an inhibitory effect on SARS-CoV-2 replication. This antiviral activity was blocked by pharmacologic inhibition of iNOS. IFN-γ also triggered a NO-mediated antiviral activity in SARS-CoV-2 infected human lung epithelial cell lines A549-ACE2 and Calu-3. IL-1β enhanced IFN-γ induction of NO, but it had little effect on antiviral activity.DiscussionGiven that IFN-g has been shown to be produced by CD8+ T cells in the early response to SARS-CoV-2, our findings in human lung epithelial cell lines, of an IFN-γ-triggered, NO-dependent, links the adaptive immune response to an innate antiviral pathway in host defense against SARS-CoV-2. These results underscore the importance of IFN-γ and NO in the antiviral response and provide insights into potential therapeutic strategies for COVID-19.

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Section: Discussionmentioning

confidence: 99%

IFN-γ-mediated control of SARS-CoV-2 infection through nitric oxide

Silva,

Krogstad,

Teles

et al. 2023

Front. Immunol.

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“…Numerous studies have shown that IFN-λ, being a nonspecific activator of a large group of ISGs, exhibits antiviral activity against a whole group of negative strand ssRNA viruses: IVA [18,21,[33][34][35][36][37][38]; IBV [39]; RSV [2,40,41]; and LCMV [42]. Some positive strand ssRNA viruses are also affected, such as: HCV [14,[43][44][45]; Dengue virus [46,47]; and rhinovirus [48,49].…”

Section: Discussionmentioning

confidence: 99%

IFN-λ1 Displays Various Levels of Antiviral Activity In Vitro in a Select Panel of RNA Viruses

Plotnikova

Lozhkov

Romanovskaya-Romanko

et al. 2021

Viruses

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Type III interferons (lambda IFNs) are a quite new, small family of three closely related cytokines with interferon-like activity. Attention to IFN-λ is mainly focused on direct antiviral activity in which, as with IFN-α, viral genome replication is inhibited without the participation of immune system cells. The heterodimeric receptor for lambda interferons is exposed mainly on epithelial cells, which limits its possible action on other cells, thus reducing the likelihood of developing undesirable side effects compared to type I IFN. In this study, we examined the antiviral potential of exogenous human IFN-λ1 in cellular models of viral infection. To study the protective effects of IFN-λ1, three administration schemes were used: ‘preventive’ (pretreatment); ‘preventive/therapeutic’ (pre/post); and ‘therapeutic’ (post). Three IFN-λ1 concentrations (from 10 to 500 ng/mL) were used. We have shown that human IFN-λ1 restricts SARS-CoV-2 replication in Vero cells with all three treatment schemes. In addition, we have shown a decrease in the viral loads of CHIKV and IVA with the ‘preventive’ and ‘preventive/therapeutic’ regimes. No significant antiviral effect of IFN-λ1 against AdV was detected. Our study highlights the potential for using IFN-λ as a broad-spectrum therapeutic agent against respiratory RNA viruses.

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“…As a potent antiviral molecule, IFN-λs are capable of a variety of functions against: Hepatitis C virus, 29 Hepatitis B virus, 30 Herpes viruses, [31][32][33][34] Human immunodeficiency virus, 35 Dengue virus, 36 Zika virus, 37 SARS corona virus, 38,39 Influenza virus, 40 human metapneumovirus, 41 Lymphocytic choriomeningitis virus, 42 Norovirus, 43 Rhinovirus, 44 and many others. In addition to viral diseases, IFN-λs have a wide variety of actions in reduction and control of bacterial, fungal, and parasitic infections.…”

Section: Ifn-λs Influence On Medical Diseases and Disordersmentioning

confidence: 99%

Interferon Lambda: An Anonymous Warrior of Host-Microbial Fray in The Pathogenesis of Periodontal Diseases

Ahmed

2024

Bangladesh J Med Sci

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The current body of research focuses primarily on three fundamental groups of interferons: alpha, beta, and gamma interferons. However, little attention is paid to another important group of interferons called lambda interferons (IFN-λs), which have demonstrated activity against periodontal pathogens, particularly viruses. These recently discovered interferon-lambdas provide a striking contrast to the periodontal pathogens, exerting impact both at the capacity of the immune system and the cell, thereby affecting a variety of inflammatory and response pathways. Thus, this review sheds light on role of interferon lambda in the development of periodontal disease and associated activities. In order to collect the information necessary for this review, an exhaustive search of the relevant literature was conducted. The review focused on the structure, regulatory mechanisms, and role of interferon lambda in medical and periodontal infections and diseases. The findings of our review indicated that IFN-λs have an effect on the immunological regulatory mechanisms of the host in relation to viruses, bacteria, fungi, and parasites; nevertheless, their primary role is antiviral, and they have little to no effect on the immune system's ability to fight bacteria. Bangladesh Journal of Medical Science Vol.23 (Special Issue) 2024 p.S73-S78

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Antiviral Effect of Interferon Lambda Against Lymphocytic Choriomeningitis Virus

Cited by 12 publications

References 75 publications

IFN-γ-mediated control of SARS-CoV-2 infection through nitric oxide

IFN-γ-mediated control of SARS-CoV-2 infection through nitric oxide

IFN-λ1 Displays Various Levels of Antiviral Activity In Vitro in a Select Panel of RNA Viruses

Interferon Lambda: An Anonymous Warrior of Host-Microbial Fray in The Pathogenesis of Periodontal Diseases

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