Antiviral effect of baicalin on Marek’s disease virus in CEF cells

Abstract

Background Baicalin, the main metabolic component of Scutellaria baicalensis Georgi, has various pharmacological properties including anti-inflammatory, anti-oxidant, anti-apoptotic, anti-bactericidal and anti-viral. The purpose of this study was to investigate the anti-Marek’s disease virus (MDV) activities of baicalin in CEF cells. Results Here, we showed that baicalin could inhibit viral mRNA, protein levels and overall plaque formation in a time-dependent manner. We also found that baicalin could consistently inhibit MDV replication and directly affect the virus infectivity. Moreover, baicalin treatment has no effect on expression level of antiviral cytokine and inflammatory cytokines in MDV infected CEFs. Conclusions These results demonstrate that baicalin could be a potential drug against MDV infection.

Effects of Xiaochaihu decoction on the expression of cytochrome P450s in rats

“…As a traditional Chinese herbal medicine, Scutellariae Radix exhibits important effects in the treatment of various diseases, including emesis, hepatitis and high blood pressure. Baicalin, the main bioactive ingredient in Scutellariae Radix, exerts antidepressant ( 21 ), anti-inflammatory and antioxidant effects ( 22 ). Therefore, baicalin content is determined for quality control of many traditional Chinese medicines containing Scutellariae Radix ( 23 ).…”

Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts

“…In order to study the mechanism of LiCl inhibition on MDV infection, three time points related to the addition of LiCl (P1: before MDV (1000 pfu) infection, P2: during MDV infection, and P3: after MDV infection) were used, similar to our previous report [21]. The results of real-time PCR and plaque counts showed that the application of 20 mM LiCl had a significant inhibitory effect on MDV replication at all time points (Figure 3).…”

“…In order to study the mechanisms of LiCl inhibition of the virus, experiments related to the time of addition of LiCl were carried out as described previously [21]. Briefly, in the first experimental protocol (P1), CEF cells were pretreated with LiCl (20 mM) at 37 • C for 2 h, and the medium containing LiCl was removed before inoculation of 1000 PFU of virus.…”

“…Similar to the protocols described in our previous report [21], 1000 PFU of virus stock was directly mixed with 20 mM LiCl in a tube and incubated at 37 • C for 1.5 h. After centrifugation to remove the supernatant, the virus-infected cells were resuspended and added to the CEF in a 12-well plate. After 4 h of virus attachment, the inoculum was replaced with fresh maintenance medium containing 0.5% serum.…”

“…As described in our previous report [21], trypsinised primary CEF cells were pated into each well of 96-well plate. The next day, 10-fold dilutions of the virus-containing cell suspension were added to the CEF, with 12 replicates of each dilution.…”