Antiviral, antifungal, and insecticidal activities of Eucalyptus bark extract: HPLC analysis of polyphenolic compounds

Abdelkhalek, Ahmed; Salem, M. H.; Kordy, Ahmed; Salem, Abdelfattah Z. M.; Behiry, Said I.

doi:10.1016/j.micpath.2020.104383

Cited by 60 publications

(40 citation statements)

References 69 publications

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“…Bacterial isolation phenotypic identification and molecular characterization. By using the halfleaf method 28 , the most potent bacterial isolate exhibiting antiviral activity was selected and subject for morphological, physiological, biochemical characteristics (Table 1) and molecular identification. Based on the bacterial phenotypic characteristics and nucleotide sequencing analysis of the amplified 16S rRNA gene, the selected bacterial isolate was identified as Bacillus licheniformis strain POT1, and the annotated sequence was submitted to GenBank database under the accession number MT077309.…”

Section: Resultsmentioning

confidence: 99%

Bacillus licheniformis strain POT1 mediated polyphenol biosynthetic pathways genes activation and systemic resistance in potato plants against Alfalfa mosaic virus

Abdelkhalek

Al-Askar

Behiry

2020

Sci Rep

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Alfalfa mosaic virus (AMV) is a worldwide distributed virus that has a very wide host range and causes significant crop losses of many economically important crops, including potato (Solanum tuberosum L.). In this study, the antiviral activity of Bacillus licheniformis strain POT1 against AMV on potato plants was evaluated. The dual foliar application of culture filtrate (CF), 24 h before and after AMV-inoculation, was the most effective treatment that showed 86.79% reduction of the viral accumulation level and improvement of different growth parameters. Moreover, HPLC analysis showed that a 20 polyphenolic compound was accumulated with a total amount of 7,218.86 and 1606.49 mg/kg in POT1-treated and non-treated plants, respectively. Additionally, the transcriptional analysis of thirteen genes controlling the phenylpropanoid, chlorogenic acid and flavonoid biosynthetic pathways revealed that most of the studied genes were induced after POT1 treatments. The stronger expression level of F3H, the key enzyme in flavonoid biosynthesis in plants, (588.133-fold) and AN2, anthocyanin 2 transcription factor, (97.005-fold) suggested that the accumulation flavonoid, especially anthocyanin, might play significant roles in plant defense against viral infection. Gas chromatography-mass spectrometry (GC-MS) analysis showed that pyrrolo[1,2-a]pyrazine-1,4-dione is the major compound in CF ethyl acetate extract, that is suggesting it acts as elicitor molecules for induction of systemic acquired resistance in potato plants. To our knowledge, this is the first study of biological control of AMV mediated by PGPR in potato plants.

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Section: Resultsmentioning

confidence: 99%

Bacillus licheniformis strain POT1 mediated polyphenol biosynthetic pathways genes activation and systemic resistance in potato plants against Alfalfa mosaic virus

Abdelkhalek

Al-Askar

Behiry

2020

Sci Rep

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“…The ZnO NPs were suspended in sterile distilled water at 10 mg/mL, and then diluted with sterile distilled H 2 O to obtain a final concentration of 100 µg/mL. By using the half-leaf method [41,42], the antiviral activity of ZnO NPs was primary tested on Datura stramonium, as a local lesion host for TMV, according to the inhibition percentage towards the number of local lesions. Under insect-proof greenhouse conditions, tomato seeds were surface sterilized and cultivated in plastic pots (20 cm in diameter) filled with sterilized soil (clay: sand, 1:1).…”

Section: Greenhouse Experimental Design and Antiviral Activity Assaymentioning

confidence: 99%

“…Beside it is the first enzyme in the phenylpropanoid pathway linking the primary and secondary metabolism [61], PAL is involved in salicylic acid (SA) biosynthesis [62]. SA is an important signal that regulates systemic acquired resistance (SAR) in plants [42,63] and its role in plant immunity has been investigated for decades. The activation of SA, in response to pathogens, results in the accumulation and overexpression of PR-1 (SA marker gene) [64].…”

Section: Transcriptional Levels Of Defense-related Genesmentioning

confidence: 99%

Green Synthesized ZnO Nanoparticles Mediated by Mentha Spicata Extract Induce Plant Systemic Resistance against Tobacco Mosaic Virus

2020

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Globally, plant viral infection is one of the most difficult challenges of food security, where considerable losses in crop production occur. Nanoparticles are an effective control agent against numerous plant pathogens. However, there is limited knowledge concerning their effects against viral infection. In the present study, the green synthesis of zinc oxide nanoparticles (ZnO NPs) using aqueous leaf extract of Mentha spicata was achieved. X-ray diffraction patterns confirmed the crystalline nature of the prepared ZnO NPs. Dynamic light scattering and scanning electron microscopy analyses revealed that the resultant ZnO NPs were spherical in shape with a particle size ranged from 11 to 88 nm. Fourier transmission infrared spectroscopy detected different functional groups, capping and stability agents, and showed Zn-O bond within wavenumber of 487 cm−1. Under greenhouse conditions, the antiviral activity of biological synthesized ZnO NPs (100 µg/mL) against Tobacco mosaic virus (TMV) was evaluated. The double foliar application of the prepared ZnO NPs, 24 h before and 24 h after TMV-inoculation, was the most effective treatment that showed a 90.21% reduction of viral accumulation level and disease severity. Additionally, the transcriptional levels of PAL, PR-1 (salicylic acid marker gene), CHS, and POD genes were induced and up-regulated in all ZnO NPs treated plants. Notably, the results exhibited that aqueous extract of Mentha spicata was an effective reducing agent for the green synthesis of ZnO NPs, which showed significant antiviral activity. Finally, the detected protective and curative activity of ZnO NPs against TMV can encourage us to recommend its application for plant viral disease management. To our knowledge, this is the first study describing the antiviral activity of the green synthesized ZnO NPs.

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“…harzianum and A. niger without changing the wood structures, 84 Leucaena leucocephala wood treated with Acer saccharum var. saccharum extract from inner or outer bark extract in combination with citric acid showed bioactivity against the growth of T. viride, F. subglutinans, and A. niger, 6 Melia azedarach wood treated with E. camaldulensis or V. agenus-castus n-hexane oily extracts showed potential antifungal against F. culmorum, Rhizoctonia solani and Penicillium chrysogenum, 7 M. azedarach wood samples treated with A. saligna owers extract showed promising antifungal activity against P. chrysogenum, 4 M. azedarach wood treated with Musa paradisiaca extract showed good bioactivity against F. culmorum and R. solani, 5 treated-chinaberry wood blocks with E. camaldulensis bark extract shpwed potential antifungal activity against F. culmorum and Botrytis cinerea, 85 and M. azedarach wood treated with whole plant extract Haplophyllum tuberculatum observed good antifungal activity against F. culmorum and R.…”

Section: Discussionmentioning

confidence: 98%

“…saligna wood treated with of Cupressus sempervirens methanolic extract against Trichoderma harzianum infestation,3 treated wood samples from Pinus sylvestris, P. rigida and Fagus sylvatica with two EOs of P. rigida (wood) or Eucalyptus camaldulensis (leaves) showed promising antifungal activity against ve molds (A. alternata, F. subglutinans, C. globosum, A. niger, and T. viride),50 treated wood samples from A. saligna, F. sylvatica, Juglans nigra and P. rigida the oil from Origanum showed good antifungal activity against T. harzianum and A. niger without changing the wood structures,84 Leucaena leucocephala wood treated with Acer saccharum var. saccharum extract from inner or outer bark extract in combination with citric acid showed bioactivity against the growth of T. viride, F. subglutinans, and A. niger,6 Melia azedarach wood treated with E. camaldulensis or V. agenus-castus n-hexane oily extracts showed potential antifungal against F. culmorum, Rhizoctonia solani and Penicillium chrysogenum, 7 M. azedarach wood samples treated with A. saligna owers extract showed promising antifungal activity against P. chrysogenum, 4 M. azedarach wood treated with Musa paradisiaca extract showed good bioactivity against F. culmorum and R. solani,5 treated-chinaberry wood blocks with E. camaldulensis bark extract shpwed potential antifungal activity against F. culmorum and Botrytis cinerea,85 and M. azedarach wood treated with whole plant extract Haplophyllum tuberculatum observed good antifungal activity against F. culmorum and R.…”

mentioning

confidence: 99%

Mentha longifolia and Citrus reticulata Essential Oils: Potential wood-biofungicide and MNDO Quantum Chemical Studies

Ali¹,

Elgat²,

EL-Hefny³

et al. 2020

Preprint

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Essential oils (EOs) from aerial parts from Mentha longifolia (Saudi cultivar) and Citrus reticulata grown in Egypt, were analyzed by GC/MS to identify their chemical compounds. The EOs were tested for their antifungal activity against four common fungi Aspergillus flavus, A. niger, A. fumigatus, and Fusarium culmorum. The most abundant compounds identified in the EO from M. longifolia were menthone, and eucalyptol with percentages of 48.00%, and 21.66%, respectively. The main chemical compounds from C. reticulata EO were β-caryophyllene, β-caryophyllene oxide, and β-elemene with values of 15.57%, 7.04%, and 6.39%, respectively. At the concentrations of 500 and 250 µL/mL, the EOs from M. longifolia and C. reticulata showed potent antifungal activity against A. flavus and A. fumigatus with 100% fungal mycelial inhibition growth (FMIG). FMIG with values of 98% and 95% were observed against A. fumigatus by C. reticulata and M. longifolia EOs, respectively, at 125 µL/mL. At 500 and 250 µL/mL levels of concentration from the EO of M. longifolia, potent antifungal activity found against A. niger with 100% FMIG. F. culmorum completely inhibited (100% FMIG) as EOs from M. longifolia and C. reticulata applied at 500 µL/mL. Pinus halepensis wood treated with M. longifolia at 125 µL/mL showed inhibition zone values of 7.33 mm, 21.33 mm A. flavus and Aspergillus niger, respectively. The results suggested the potential values of the EOs as potent antifungal activity against some common molds that grows over wood packaging during transportation of vegetables and fruits or can cause food spoilage.

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Antiviral, antifungal, and insecticidal activities of Eucalyptus bark extract: HPLC analysis of polyphenolic compounds

Cited by 60 publications

References 69 publications

Bacillus licheniformis strain POT1 mediated polyphenol biosynthetic pathways genes activation and systemic resistance in potato plants against Alfalfa mosaic virus

Bacillus licheniformis strain POT1 mediated polyphenol biosynthetic pathways genes activation and systemic resistance in potato plants against Alfalfa mosaic virus

Green Synthesized ZnO Nanoparticles Mediated by Mentha Spicata Extract Induce Plant Systemic Resistance against Tobacco Mosaic Virus

Mentha longifolia and Citrus reticulata Essential Oils: Potential wood-biofungicide and MNDO Quantum Chemical Studies

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