Antiviral and Anti-inflammatory Diterpenoids from the Soft Coral <i>Sinularia gyrosa</i>

Cheng, Shi‐Yie; Chuang, Cheng-Ta; Wang, Shang‐Kwei; Wen, Zhi‐Hong; Chiou, Shu‐Fen; Hsu, Chi‐Hsin; Dai, Chang‐Feng; Duh, Chang‐Yih

doi:10.1021/np100185a

Cited by 44 publications

(28 citation statements)

References 23 publications

(56 reference statements)

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“…Previous studies have indicated that diterpenes, a main constituent of the genus Sinularia , exhibit various biological activities, such as anti-inflammatory (Chao et al 2011; Cheng et al 2010; Lu et al 2010; Su and Wen 2011), antiviral (Cheng et al 2010), and cytotoxic (Grote et al 2008; Kamel et al 2007; Lo et al 2009; Su et al 2009) effects. As a part of our ongoing investigations on screening active compounds from Vietnamese Sinularia soft corals towards anti-inflammatory effects (Thao et al 2012, 2013a, b), we recently reported the isolation, structure elucidation, and inhibitory effects on lipopolysaccharide-stimulated production of proinflammatory cytokines in bone marrow-derived dendritic cells of 12 diterpenoids [sinumaximol A ( 1 ), sinumaximol B ( 2 ), sinumaximol C ( 3 ), sethukarailin ( 4 ), sinumaximol D ( 5 ), sinumaximol E ( 6 ), sinumaximol F ( 7 ), sinumaximol G ( 8 ), sinumaximol H ( 9 ), (1 S ,2 E ,4 S ,6 E ,8 S ,11 R )-2,6,12(20)-cembratriene-4,8,11-triol ( 10 ), isomandapamate ( 11 ), and sinumaximol I ( 12 )] (Thao et al 2012) and 7 norditerpenoids [scabrolide A ( 13 ), 12-hydroxy-scabrolide A ( 14 ), yonarolide ( 15 ), ineleganolide ( 16 ), 5-epinorcembrene ( 17 ), 13- epi -scabrolide C ( 18 ), and norcembrene 5 ( 19 )] (Thao et al 2013a) from Sinularia maxima (see Fig.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of NF-κB transcriptional activation in HepG2 cells by diterpenoids from the soft coral Sinularia maxima

et al. 2013

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Anti-inflammatory transcriptional effects of nineteen compounds (1–19) from the soft coral Sinularia maxima were evaluated using NF-κB luciferase and reverse transcriptase polymerase chain reaction. Compounds 1, 2, 4, 8, 15, 17, and 18 significantly inhibited TNFα-induced NF-κB transcriptional activity in HepG2 cells in a dose-dependent manner, with IC50 values ranging from 15.81 ± 2.29 to 29.10 ± 1.54 μM. Furthermore, the transcriptional inhibitory function of these compounds was confirmed by a decrease in intercellular adhesion molecule-1 and inducible nitric oxide synthase gene expression levels in HepG2 cells. These results provide a scientific rationale for the use of the soft coral S. maxima warrant further studies to develop new agents for the prevention and treatment of inflammatory.

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Section: Introductionmentioning

confidence: 99%

Inhibition of NF-κB transcriptional activation in HepG2 cells by diterpenoids from the soft coral Sinularia maxima

et al. 2013

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“…Cell Western blot analysis. Cells were treated with 0, 5, 20 and 80 µmol/l PAB solution for 24 h. The cells were then harvested (cell number, >5x10 6 /ml) and washed twice with cold PBS. Western blot analysis was performed.…”

Section: Assessment Of Morphological Changes A549 Cells (50x10mentioning

confidence: 99%

“…Diterpene acid is one of the numerous traditional Chinese medicines that has a long history of recordation and clinical application (5). Diterpene acid has antiviral, anti-inflammatory, antitumor, antifungal and immunosuppressive effects (6)(7)(8)(9). Pseudolaric acid B (PAB; Fig.…”

Section: Introductionmentioning

confidence: 99%

Role of pseudolaric acid B in A549 lung cancer cell proliferation and apoptosis

Guan

Yang

2013

Molecular Medicine Reports

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Abstract. Recently, traditional Chinese medicine has gained attention for its potential use as a chemotherapeutic agent. Pseudolaric acid B (PAB) is a diterpene acid isolated from Pseudolarix kaempferi and possesses antifungal, antimicrobial, antifertility and antiangiogenic properties. It was also reported that PAB may inhibit proliferation and induce apoptosis in various types of cancer. However, its effects on A549 lung cancer cells remain to be determined. The present study aimed to determine the potential roles of PAB in the proliferation and apoptosis of A549 cells. The results showed that PAB inhibited A549 cell proliferation in a time-and dose-dependent manner. Fluorescence microscopy results showed that cells treated with 20 µmol/l PAB for 24 h exhibited karyorrhexis and apoptotic body formation. In addition, A549 cells were treated with 5, 10, 20, 40 or 80 µmol/l PAB for 24 h and apoptosis was analyzed using Annexin-V/propidium iodide kit. The apoptosis rates were 8. 95, 18.71, 24.66, 35.02 and 43.64%, respectively, in PAB-treated cells and 0.80% in the control group. Furthermore, western blot analysis showed that PAB treatment upregulated the protein levels of Bax, Bad and downregulated Bcl-2 and Bcl-xl expression. In conclusion, PAB may serve as a potent chemotherapeutic agent against human lung cancer.

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“…The genera of Sinularia and Lobophytum (Phylum: Coelenterata, Family: Alcyoniidae, Class: Anthozoa, Order: Alcyonacea) are two of the most widely distributed of their genera, constituting a dominant portion of the biomass in tropical reef environments (Anjaneyulu and Rao, 1997;Lakshmi and Kumar, 2009). Previous studies have indicated that diterpenes, a main constituent of the genus Sinularia and Lobophytum (most are derived from the 14-membered cembrane nucleus), exhibit various biological activities, such as anti-inflammatory (Chao et al, 2011;Cheng et al, 2010;Lu et al, 2010;Su and Wen, 2011), antiviral (Cheng et al, 2010), and cytotoxic (Grote et al, 2008;Kamel et al, 2007;Lo et al, 2009;Su et al, 2009) effects.…”

Section: Introductionmentioning

confidence: 99%

Antiosteoporotic and antioxidant activities of diterpenoids from the Vietnamese soft corals Sinularia maxima and Lobophytum crassum

et al. 2015

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Marine organisms possess bioactive potential with tremendous pharmaceutical promise. The purpose of the present study was to investigate the antioxidant (peroxyl radical-scavenging and reducing capacities) and antiosteoporotic activities of isolated constituents (1-26) from the soft corals Sinularia maxima and Lobophytum crassum on pre-osteoblastic MC3T3-E1 cells and pre-osteoclastic RAW 264.7 cells. Among the isolated compounds, 7, 9, 17, 18, and 20 (10.0 lM) exhibited significant peroxyl radical-scavenging capacity, with TE values ranging from 4.00 ± 0.14 to 9.06 ± 0.33 lM. In addition, compounds 1, 5, 7, 20, and 24 significantly stimulated the differentiation of pre-osteoblastic MC3T3-E1 cells by increasing collagen synthesis and/or mineralization functions of osteoblasts, while compounds 1, 5-8, 12, 14-22, 24, and 26 showed significant inhibition of TRAP in NFjB ligand-induced osteoclastic RAW 264.7 cells, with values ranging from 99.11 ± 1.36 to 61.54 ± 1.61 %. These results indicate that the soft corals S. maxima and L. crassum are excellent sources among the antioxidant and antiosteoporotic marine invertebrates.

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Antiviral and Anti-inflammatory Diterpenoids from the Soft Coral Sinularia gyrosa

Cited by 44 publications

References 23 publications

Inhibition of NF-κB transcriptional activation in HepG2 cells by diterpenoids from the soft coral Sinularia maxima

Inhibition of NF-κB transcriptional activation in HepG2 cells by diterpenoids from the soft coral Sinularia maxima

Role of pseudolaric acid B in A549 lung cancer cell proliferation and apoptosis

Antiosteoporotic and antioxidant activities of diterpenoids from the Vietnamese soft corals Sinularia maxima and Lobophytum crassum

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