Antiviral Activity of Some Plants Used in Nepalese Traditional Medicine

Rajbhandari, Meena; Mentel, Renate; Jha, Pramod Kumar; Chaudhary, Ram Prasad; Bhattarai, Shandesh; Gewali, Mohan B.; Karmacharya, Neena; Hipper, Marja; Lindequist, Ulrike

doi:10.1093/ecam/nem156

Cited by 134 publications

(31 citation statements)

References 26 publications

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“…diabetes 7 and it was also found to have antiviral activity against Influenza virus A. 8 Our survey revealed that the polyherbal formulation (HP) is made by mixing the roots of both the plants by an herbal practitioner for the treatment of heart palpitation. However the medicine prepared by traditional methods may not have the desired quality and batch to batch consistency.…”

Section: Introductionmentioning

confidence: 96%

Pharmacognostic Studies and In Vitro Antioxidant Potential of Traditional Polyherbal Formulation of West Sikkim with Asparagus Spp

Subba¹,

Mandal²

2015

Phcog J

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Introduction:The powder mixture of the two species of Asparagaceae (Asparagus filicinus and Asparagus officinalis) was found to be used traditionally for the treatment of heart palpitation in west Sikkim. Objective: Pharmacognostic characterisation was carried out for the authentication of the powder drug which included powder microscopy, fluorescence analysis and physicochemical characterisation. The presence of any therapeutic potential in HP was also determined by qualitative and quantitative estimation of phytochemicals along with free radical scavenging activity of various successive solvent extracts (based on their polarity). Thin layer chromatography (TLC) of the powdered HP was also done. The standard software SPSS (ver. 15.0) and XLSTAT 2009 (Addinsoft) and Smith's Statistical Package were used for different statistical analysis. Results: Powder microscopy of HP revealed the presence of calcium oxalate crystal, tracheids, stone cells etc. Various fluorescence colours were exhibited by HP on UV after reacting with different chemical reagents. The analysis values were also obtained in a satisfactory way. TLC and qualitative phytochemical analysis revealed the presence of some active phytoconstituents. Among all the solvent extracts, acetone, heptane, ethyl acetate and benzene extracts showed higher antioxidant potential. Conclusion: The results support the use of HP as a traditional medicine and further purification should be done for the identification of bioactive phytoconstituents responsible for its antioxidant activity.Key words: Antioxidant, Pharmacognostic evaluation, Phytoconstituents, Successive solvent extraction, Thin layer Chromatography. SUMMARY• HP powder prepared from the roots of Asparagus officinalis and Asparagus filicinus is used to cure heart palpitation in the traditional system at a village in west Sikkim.• Powder was subjected for some pharamacognostic characterisation methods and the result of which might be helpful in authentication of the traditional drug.• Some phytoconstituents such as anthraglycosides, arbutin, cardiac glycosides, flavonoids, bitter principles and coumarins were revealed on the sample by separating through thin layer chromatography. Alkaloids and saponins were absent. • Qualitative evaluation of phytochemicals revealed presence of cardiac glycosides and flavonoids in all solvent extracts while amino acid and triterpenoids in few extracts only and showed absence of tannin, resin and alkaloids.• Antioxidants potential varied in different solvent extracts but overall acetone and heptane extracts were better than other extracts.

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Section: Introductionmentioning

confidence: 96%

Pharmacognostic Studies and In Vitro Antioxidant Potential of Traditional Polyherbal Formulation of West Sikkim with Asparagus Spp

Subba¹,

Mandal²

2015

Phcog J

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“…Medicinal plants because of their potential health effects have become part of complementary medicine worldwide (2). Various plant extracts have been widely used for therapeutic purposes, including battling infectious diseases (3). Verbascum L. is a member of the family Scrophulariaceae represented by 5,100 species, all of which are grown in temperate and tropical areas (4).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Aqueous-Alcoholic Extracts and the Essential Oil of Verbascum thapsus L.

Ghasemi

Rezaei

Araghi

et al. 2015

Jundishapur J Nat Pharm Prod

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Background:The increasing resistance of human pathogens to the available antimicrobials is a serious threat, resulting in the need for novel antibiotic resources such as plants. Some species of the genus Verbascum have been used by mankind since ancient times as an effective remedy for infectious diseases. Objectives: This study was designed to determine the antimicrobial efficacy of the aqueous-alcoholic extracts and the essential oil of Verbascum thapsus L. against different kinds of bacterial and fungal strains, viz. Streptococcus pyogenes, Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus fumigatus. Materials and Methods:The antimicrobial activities of the V. thapsus extracts were examined in the present study on the basis of disc diffusion and microdilution assays, and their potency was quantitatively assessed in terms of inhibition zone diameters and minimum inhibitory concentration (MIC) values. Results:The disk diffusion test showed that the methanol extract of V. thapsus had more growth inhibitory effects on E. coli and S. pyogenes than the aqueous and ethanol extracts. The methanol and aqueous extracts had no effects on S. aureus. The maximal inhibition zone for the microorganisms sensitive to the methanol extract was in the range of 7 -16.8 mm, and the MIC value was 31.25 μg/mL. For the ethanol extract, the maximal inhibition zone was 5.3 -11 mm and the MIC value was 62.5 -125 μg/mL. The essential oil of V. thapsus did not exhibit any antibacterial and antifungal activities. Conclusions:The findings of the present study revealed the V. thapsus extract possesses compounds with antibacterial properties that can be used as novel antimicrobial agents in the development of new drugs for the treatment of infectious diseases.

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“…Besides ethnobotanical studies, in-vitro and in-vivo trials were realized to identify the mechanisms explaining the effectiveness of some of the medicinal plants used in traditional medicine (Griggs et al, 2001;Panthi and Chaudhary, 2006;Rajbhandari et al, 2009). This practice of establishing phytochemical or pharmacological explanations for traditional uses is not only helpful to institutionalize traditional medicine, but can also lead to the development of new drugs (Newman and Cragg, 2007) or indicate future directions for bio-prospecting (Soejarto et al, 2005;Douwes et al, 2008).…”

Section: Introductionmentioning

confidence: 99%