Antiviral activity of silver nanoparticle/chitosan composites against H1N1 influenza A virus

Mori, Yasutaka; Ono, Takeshi; Miyahira, Yasushi; Nguyen, Vinh; Matsui, Takemi; Ishihara, Masayuki

doi:10.1186/1556-276x-8-93

Cited by 290 publications

(201 citation statements)

References 26 publications

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“…Furthermore, the performed studies of antiviral action of various nanoparticles show that the higher the nanoparticle concentration, the higher their antiviral and antimicrobial effect [8][9][10][11][12]. Thus, the results are in good agreement with the results of biomedical experiments.…”

Section: Semiconductor Physics Quantumsupporting

confidence: 65%

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Possible method for evaluation of virus, bacteria and yeasts infectivity by optical measurements

Rusinchuk¹

2016

Semicond. Phys. Quantum Electron. Optoelectron.

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Abstract. The possible method for evaluation of virus, bacteria and yeasts infectivity based on study of their absorption spectra has been proposed. The decrease in the samples infectivity was caused by adding the CeO 2 nanoparticles. Optical absorption of the samples was measured for the wavelength range from 400 to 750 nm. The obtained data detect the specific changes in absorption of the samples, which can be used for development of the new technique of evaluation of virus, bacteria and yeasts infectivity.

show abstract

Section: Semiconductor Physics Quantumsupporting

confidence: 65%

“…It is well known that the samples with nanoparticles have antiviral and antimicrobial effect [8][9][10][11][12]. Furthermore, the performed studies of antiviral action of various nanoparticles show that the higher the nanoparticle concentration, the higher their antiviral and antimicrobial effect [8][9][10][11][12].…”

Section: Semiconductor Physics Quantummentioning

confidence: 99%

Possible method for evaluation of virus, bacteria and yeasts infectivity by optical measurements

Rusinchuk¹

2016

Semicond. Phys. Quantum Electron. Optoelectron.

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“…Park et al (2014) demonstrated that magnetic hybrid colloid decorated with AgNP (AgNP@MHCs) is able to inactivate bacteriophage ΦX174, murine norovirus (MNV), and adenovirus serotype 2 (AdV2). Mori et al (2013) proved antiviral activity of AgNP/chitosan composites against H1N1 influenza A virus. Lara et al (2010) reported that AgNP exert anti-viral activity against HIV-1 at an early stage of viral replication, since AgNP may act as inhibitors of viral entry.…”

Section: Den-2 Growth Inhibition Assaysmentioning

confidence: 95%

Green-synthesized silver nanoparticles as a novel control tool against dengue virus (DEN-2) and its primary vector Aedes aegypti

et al. 2015

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Dengue is an arthropod-borne viral infection mainly vectored through the bite of Aedes mosquitoes. Recently, its transmission has strongly increased in urban and semi-urban areas of tropical and sub-tropical regions worldwide, becoming a major international public health concern. There is no specific treatment for dengue. Its prevention and control solely depends on effective vector control measures. In this study, we proposed the green-synthesis of silver nanoparticles (AgNP) as a novel and effective tool against the dengue serotype DEN-2 and its major vector Aedes aegypti. AgNP were synthesized using the Moringa oleifera seed extract as reducing and stabilizing agent. AgNP were characterized using a variety of biophysical methods including UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and sorted for size categories. AgNP showed in vitro antiviral activity against DEN-2 infecting vero cells. Viral titer was 7 log10 TCID50/ml in control (AgNP-free), while it dropped to 3.2 log10 TCID50/ml after a single treatment with 20 μl/ml of AgNP. After 6 h, DEN-2 yield was 5.8 log10 PFU/ml in the control, while it was 1.4 log10 PFU/ml post-treatment with AgNP (20 μl/ml). AgNP were highly effective against the dengue vector A. aegypti, with LC50 values ranging from 10.24 ppm (I instar larvae) to 21.17 ppm (pupae). Overall, this research highlighted the concrete potential of green-synthesized AgNP in the fight against dengue and its primary vector A. aegypti. Further research on structure-activity relationships of AgNP against other dengue serotypes is urgently required.

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“…11,12 AgNPs are increasingly being explored as antiviral agents in viral illnesses, such as human immunodeficiency virus-1, hepatitis B virus, herpes simplex virus type 1, Monkeypox virus, and Tacaribe virus. [13][14][15][16][17] The mechanisms by which AgNPs exert their antiviral effects remain unclear.…”

mentioning

confidence: 99%

Inhibition of A/Human/Hubei/3/2005 (H3N2) influenza virus infection by silver nanoparticles in vitro and in vivo

Xiang¹,

Zheng²,

Duan³

et al. 2013

IJN

173

114

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Silver nanoparticles (AgNPs) have attracted much attention as antimicrobial agents and have demonstrated efficient inhibitory activity against various viruses, including human immunodeficiency virus, hepatitis B virus, and Tacaribe virus. In this study, we investigated if AgNPs could have antiviral and preventive effects in A/Human/Hubei/3/2005 (H3N2) influenza virus infection. Madin-Darby canine kidney cells infected with AgNP-treated H3N2 influenza virus showed better viability ( P <0.05 versus influenza virus control) and no obvious cytopathic effects compared with an influenza virus control group and a group treated with the solvent used for preparation of the AgNPs. Hemagglutination assay indicated that AgNPs could significantly inhibit growth of the influenza virus in Madin-Darby canine kidney cells ( P <0.01 versus the influenza virus control). AgNPs significantly reduced cell apoptosis induced by H3N2 influenza virus at three different treatment pathways ( P <0.05 versus influenza virus control). H3N2 influenza viruses treated with AgNPs were analyzed by transmission electron microscopy and found to interact with each other, resulting in destruction of morphologic viral structures in a time-dependent manner in a time range of 30 minutes to 2 hours. In addition, intranasal AgNP administration in mice significantly enhanced survival after infection with the H3N2 influenza virus. Mice treated with AgNPs showed lower lung viral titer levels and minor pathologic lesions in lung tissue, and had a marked survival benefit during secondary intranasal passage in vivo. These results provide evidence that AgNPs have beneficial effects in preventing H3N2 influenza virus infection both in vitro and in vivo, and demonstrate that AgNPs can be used as potential therapeutics for inhibiting outbreaks of influenza.

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Antiviral activity of silver nanoparticle/chitosan composites against H1N1 influenza A virus

Cited by 290 publications

References 26 publications

Possible method for evaluation of virus, bacteria and yeasts infectivity by optical measurements

Possible method for evaluation of virus, bacteria and yeasts infectivity by optical measurements

Green-synthesized silver nanoparticles as a novel control tool against dengue virus (DEN-2) and its primary vector Aedes aegypti

Inhibition of A/Human/Hubei/3/2005 (H3N2) influenza virus infection by silver nanoparticles in vitro and in vivo

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