Antiviral activity of Mx proteins from gilthead seabream (Sparus aurata) against lymphocystis disease virus (LCDV)

Fernández-Trujillo, M.A.; García-Rosado, Esther; Alonso, M. Carmen; Borrego, Juan J.; Álvarez, M. Carmen; Béjar, Julia

doi:10.1016/j.fsi.2013.03.052

Cited by 1 publication

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, Mx3 was undetectable until day 30 post-infection. Furthermore, the expression of the Mx genes fluctuated during the antiviral response [ 10 ]. Mx gene expression was shown to increase significantly after type I IFN expression was induced in Xenopus laevis , and substantially reduced frog virus 3 (FV3) replication both in vitro and in vivo [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…Overexpression of the mullet fish Mx gene ( MuMx ) confirmed the significant inhibition of viral hemorrhagic septicemia virus (VHSV) transcripts, while eliciting crucial antiviral functions against viral antigens [ 29 ]. The SauMx1 and SauMx2 of gilthead seabream have antiviral activity against lymphocystis disease virus (LCDV), although SauMx3 did not inhibit LCDV replication in CHSE-214 cells [ 10 ]. Therefore, these studies of the response of Mx gene expression to the virus indicate that the functions of Mx vary with species and isoforms.…”

Section: Discussionmentioning

confidence: 99%

“…Chickens carry one Mx gene [ 7 ], while pigs carry both Mx1 and Mx2, and humans carry MxA and MxB [ 8 ]. In fish, two Mx genes have also been identified in the European sea bass ( Dicentrarchus labrax ) [ 9 ], three ( SauMx1 , SauMx2, and SauMx3 ) in the gilthead seabream ( Sparus aurata ) [ 10 ], seven in the zebrafish ( Danio rerio ) [ 11 ], nine in both the Atlantic salmon ( Salmo salar ) [ 12 ] and rainbow trout ( Oncorhynchus mykiss ) [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…As a host restriction factor, Mx2 affected hepatitis B virus replication in humans [ 22 ]. The SauMx1 and SauMx2 proteins of Gilthead seabream showed antiviral activity against lymphocystis disease virus infection (LCDV, belonging to the Iridoviridae family) [ 10 ]. Andrias davidianus , which is one of the largest amphibian species in the world, is a significant species in terms of biological evolution and nutritional value.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Four Mx Genes Identified in Andrias davidianus and Characterization of Their Response to Chinese Giant Salamander Iridovirus Infection

Meng

Fan

Jiang

et al. 2022

Animals

View full text Add to dashboard Cite

Amphibians, including Andrias davidianus, are declining worldwide partly due to infectious diseases. The Myxovirus resistance (Mx) gene is a typical interferon (IFN)-stimulated gene (ISG) involved in the antiviral immunity. Therefore, knowledge regarding the antiviral immunity of A. davidianus can be used for improved reproduction in captivity and protection in the wild. In this study, we amplified and characterized four different A. davidianus Mx genes (adMx) and generated temporal mRNA expression profiles in healthy and Chinese giant salamander iridovirus (GSIV) infected A. davidianus by qualitative real-time PCR (qPCR). The four adMx genes ranged in length from 2008 to 2840 bp. The sequences revealed conserved protein domains including the dynamin superfamily signature motif and the tripartite guanosine-5-triphosphate (GTP)-binding motif. Gene and deduced amino acid sequence alignment revealed relatively high sequence identity with the Mx genes and proteins of other vertebrates. In phylogenetic analysis, the adMx genes clustered together, but also clustered closely with those of fish species. The four adMx genes were broadly expressed in healthy A. davidianus, but were differentially expressed in the spleen during the GSIV infection. Our results show that the adMx genes share major structural features with their homologs, suggesting similar functions to those in other species.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%