Antiviral activity of lanatoside C against dengue virus infection

Cheung, Yan Yi; Chen, Karen Caiyun; Chen, Huixin; Seng, Eng Khuan; Chu, Justin Jang Hann

doi:10.1016/j.antiviral.2014.09.007

Cited by 54 publications

(39 citation statements)

References 16 publications

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“…Considering the liver involvement in dengue pathophysiology, our infection model uses the Huh-7 hepatocellular carcinoma cell line as a virus target. It is known that hepatocytes are good targets for DENV and this cell line has been used to test antivirals against DENV (Cheung et al 2014) and anti-inflammatory products (Li et al 2012). …”

Section: Discussionmentioning

confidence: 99%

Decrease in Dengue virus-2 infection and reduction of cytokine/chemokine production by Uncaria guianensis in human hepatocyte cell line Huh-7

Mello

Valente

Wolff

et al. 2017

Mem. Inst. Oswaldo Cruz

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BACKGROUND Dengue fever may present hemorrhages and cavitary effusions as result of exacerbated immune responses. We investigated hydro-alcoholic extracts from leaves (UGL) and bark (UGB) of the medicinal species Uncaria guinanensis with respect to antiviral effects in Dengue virus (DENV) infection and in immunological parameters associated with in vivo physiopathological features.METHODS Chemical profiles from UGB or UGL were compared in thin layer chromatography and 1H nuclear magnetic resonance using flavonoid compounds and a pentacyclic oxindole alkaloid-enriched fraction as references. DENV-2-infected hepatocytes (Huh-7) were treated with extracts. Cell viability, DENV antigens and immunological factors were detected by enzyme-linked immunosorbent assay (ELISA) or flow cytometry.FINDINGS The UGL mainly differed from UGB by selectively containing the flavonoid kaempferitrin. UGB and UGL improved hepatocyte viability. Both extracts reduced intracellular viral antigen and inhibited the secretion of viral non-structural protein (NS1), which is indicative of viral replication. Reduction in secretion of macrophage migration inhibitory factor was achieved by UGB, of interleukin-6 by UGL, and of interleukin-8 by both UGB and UGL. MAINCONCLUSIONS The U. guianensis extracts presented, antiviral and immunomodulatory effects for DENV and possibly a hepatocyte-protective activity. Further studies may be performed to consider these products as potential candidates for the development of an herbal product for the future treatment of dengue.

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Section: Discussionmentioning

confidence: 99%

Decrease in Dengue virus-2 infection and reduction of cytokine/chemokine production by Uncaria guianensis in human hepatocyte cell line Huh-7

Mello

Valente

Wolff

et al. 2017

Mem. Inst. Oswaldo Cruz

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“…A cardiac glycoside, lanatoside C, was reported to exert potent antiviral effects against DENV-1-4, Kunjin virus and other RNA viruses 266 . Analysis of the mechanism of action of lanatoside C suggests that this compound possibly targets viral RNA synthesis 266 . Digoxin, another cardiac glycoside, was recently evaluated against ZIKV and displayed antiviral effects 12 .…”

Section: Polyamine Synthesis Inhibitorsmentioning

confidence: 99%

Broad-spectrum agents for flaviviral infections: dengue, Zika and beyond

Boldescu

Behnam

Vasilakis³

et al. 2017

Nat Rev Drug Discov

248

217

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Infections with flaviviruses, such as dengue, West Nile virus, and the recently re-emerging Zika virus are an increasing and probably lasting global risk. This review summarizes and comments on the opportunities for broad-spectrum agents that are active against a range of flaviviruses. Broad-spectrum activity would be particularly desirable as preparatory measure for the next flaviviral epidemic that could emerge from as-yet-unknown or neglected viruses. Potential target sites for broad-spectrum anti-flaviviral compounds include viral proteins and host mechanisms that are exploited by these viruses during entry and replication. A variety of compounds with broad-spectrum antiviral activity have already been identified by target-specific or phenotypic assays. For some other compound classes, broad-spectrum activity can be anticipated because of their mode of action and molecular target(s).

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“…In addition to evaluating compounds that are known to have antiviral activity, classes of compounds shown to inhibit other pathogens or that have cytoprotective properties have also been evaluated. These compounds include chemicals such as flavaglines (87), suramin (88,89), flavonoids (such as silymarin) (90-92), cardiac glycosides (93,94), and other compounds from existing FDA-approved drug libraries (95). While some of these compounds were effective only if given prior to CHIKV infection, all of these compounds show some efficacy against CHIKV in cell culture systems.…”

Section: Known Antimicrobial Compoundsmentioning

confidence: 99%

Vaccine and Therapeutic Options To Control Chikungunya Virus

2018

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Beginning in 2004, chikungunya virus (CHIKV) went from an endemic pathogen limited to Africa and Asia that caused periodic outbreaks to a global pathogen. Given that outbreaks caused by CHIKV have continued and expanded, serious consideration must be given to identifying potential options for vaccines and therapeutics. Currently, there are no licensed products in this realm, and control relies completely on the use of personal protective measures and integrated vector control, which are only minimally effective. Therefore, it is prudent to urgently examine further possibilities for control. Vaccines have been shown to be highly effective against vector-borne diseases. However, as CHIKV is known to rapidly spread and generate high attack rates, therapeutics would also be highly valuable. Several candidates are currently being developed; this review describes the multiple options under consideration for future development and assesses their relative advantages and disadvantages.

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Antiviral activity of lanatoside C against dengue virus infection

Cited by 54 publications

References 16 publications

Decrease in Dengue virus-2 infection and reduction of cytokine/chemokine production by Uncaria guianensis in human hepatocyte cell line Huh-7

Decrease in Dengue virus-2 infection and reduction of cytokine/chemokine production by Uncaria guianensis in human hepatocyte cell line Huh-7

Broad-spectrum agents for flaviviral infections: dengue, Zika and beyond

Vaccine and Therapeutic Options To Control Chikungunya Virus

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