Antiviral activity of doxycycline against vesicular stomatitis virusin vitro

Abstract

Doxycycline (Dox) is a tetracycline derivative with broad-spectrum antimicrobial activities that is used as an effector substance in inducible gene-expression systems. We investigated the antiviral activity of Dox against vesicular stomatitis virus (VSV) infection in cultured H1299 cells. Dox at concentrations of 1.0-2.0 μg ml(-1) significantly inhibited VSV replication and the VSV-induced cytopathic effect in dose-dependent manners, suggesting that Dox may have broader activity in inhibiting viral replication, in addition to its well-defined bacteriostatic activity. Dox exerted its antiviral effect at the early-mid stage of VSV infection, suggesting that it did not interfere with VSV infectivity, adsorption, or entry into target cells. These results indicate that Dox can inhibit VSV infection and may therefore have potential applications for the treatment of viral infections.

The Use of Antimalarial Drugs against Viral Infection

“…This hypothesis was confirmed by in vitro experiments demonstrating that the effect of DOX was directed more toward viral entry than toward viral replication [169]. Furthermore, the inhibitory effect of DOX on the replication of vesicular stomatitis virus (VSV) in different stably infected cell lines was reported [170]. The results showed significant inhibition of the replication of VSV in a dose-dependent but not cell-type dependent manner, suggesting that DOX exerted its antiviral activity at the early-mid stage of VSV infection [170].…”

“…It has been reported that these therapies could have activities beyond antimalarial activity, also against viral infectious agents. Surprisingly, in many cases, the administration of antibiotics alone or in combination with other antiviral agents showed significant antiviral activities against different types of viral infections [168][169][170][171]. Despite this evidence, the antiviral mechanisms of action have not been completely investigated.…”

“…Furthermore, the inhibitory effect of DOX on the replication of vesicular stomatitis virus (VSV) in different stably infected cell lines was reported [170]. The results showed significant inhibition of the replication of VSV in a dose-dependent but not cell-type dependent manner, suggesting that DOX exerted its antiviral activity at the early-mid stage of VSV infection [170]. These results indicated a different mode of action of DOX against VSV than that against DENV and CHIKV, suggesting that the antiviral activity of DOX could be dependent on the virus species.…”

“…Flaviviridae BVDV(surrogate HCV) Artemisinin in vitro [187,188] Dihydroartemisinin in vitro [63] HCV Artemisinin in vitro [20][21][22] Artesunate in vitro [57] Chloroquine in vitro [148][149][150] ZIKV Mefloquine in vitro [130,131] Chloroquine in vitro [140,141] Amodiaquine in vitro [140] Atovaquone in vitro [166] DENV Quinine sulfate in vitro [66] Mefloquine in vitro [131] Halofantrine in vitro [134] Doxycycline in vitro [168,178] Amodiaquine in vitro [162] CHIKV Doxycycline in vitro [169] Chloroquine in vitro [37,138,139] Atovaquone in vitro [166] Togaviridae SFV Halofantrine in vitro [134] Rhabdoviridae VSV Doxycycline in vitro [170] Orthomyxoviridae IAV Quinine sulfate in vitro [70] In vivo (mouse) [67] Mefloquine in vitro [70] Doxycycline in vivo (mouse) [171] Chloroquine in vitro [159]…”

Inhibition of Rabies Virus by 1,2,3,4,6-Penta-O-galloyl-β-d-Glucose Involves mTOR-Dependent Autophagy

“…Twelve-well plates were seeded with BHK-21 cells and cultured overnight. 10 μM PGG was added at various stages of the virus infection [ 38 ]: 1 or 2 h before RABV infection (−2, −1 h p.i. ), together with RABV infection (0 h p.i.…”