“…Antiallergenic properties are measured in vitro determining IgE content by the reverse enzyme allergosorbent, ELISA, or enzyme allergosorbent tests (Falagiani et al., ; Wigotzki, Steinhart, & Paschke, ), or in vivo by measuring indexes of spleen and thymus status, such as changes in weight, hemolysin content, changes in optical density as a result of macrophage activity, and release of histamine from mast cells (Pan et al., ). Cytotoxic activity and apoptosis have been assessed measuring cell viability and proliferation by staining, (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay, acid phosphatase assay, neutral red uptake assay, lactate dehydrogenase assay, incorporation of [ 3 H] thymine, NK assay, or JAM test (Bateman et al., ; Chalamaiah et al., ; Hagiwara, Shinoda, Fukuwatari, & Shimamura, ; Hou et al., ; Repetto, del Peso, & Zurita, ; Richardson et al., ; Rodríguez Saint‐Jean et al., ; Yang, Sinai, & Kain, ), apoptosis by the annexin V test and caspase assay (Green & Steinmetz, ; Hartmann, Wal, Bernard, & Pentzien, ), mitochondrial transmembrane potential (Hartmann et al., ; Richardson et al., ), or DNA synthesis and damage (Hsien, Ho, Lai, & Yen, ).…”