Antiviral activity and pathogenetic targets for seaweed sulfated polysaccharides in herpesvirus infections

Беседнова, Н. Н.; Макаренкова, И. Д.; Zvyagintseva, T. N.; Имбс, Т. И.; Сомова, Л. М.; Ts, Zaporozhets

doi:10.1134/s1990750816010029

Cited by 5 publications

(5 citation statements)

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“…This result suggests the capability of these SPs to avoid viral adsorption to the cell; these data were confirmed by viral penetration assays where we observed less antiviral activity after viral attachment to the cell. More recent studies have demonstrated that fucoidans exhibit their antiviral activity when the compound is present during the virus adsorption period by blocking the interaction of viruses to the cells [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effects of Sulfated Polysaccharides from Mexican Seaweeds against Measles Virus

Morán-Santibañez

Cruz‐Suárez

Ricque‐Marie

et al. 2016

BioMed Research International

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Sulfated polysaccharides (SPs) extracted from five seaweed samples collected or cultivated in Mexico (Macrocystis pyrifera, Eisenia arborea, Pelvetia compressa, Ulva intestinalis, and Solieria filiformis) were tested in this study in order to evaluate their effect on measles virus in vitro. All polysaccharides showed antiviral activity (as measured by the reduction of syncytia formation) and low cytotoxicity (MTT assay) at inhibitory concentrations. SPs from Eisenia arborea and Solieria filiformis showed the highest antiviral activities (confirmed by qPCR) and were selected to determine their combined effect. Their synergistic effect was observed at low concentrations (0.0274 μg/mL and 0.011 μg/mL of E. arborea and S. filiformis SPs, resp.), which exhibited by far a higher inhibitory effect (96% syncytia reduction) in comparison to the individual SP effects (50% inhibition with 0.275 μg/mL and 0.985 μg/mL of E. arborea and S. filiformis, resp.). Time of addition experiments and viral penetration assays suggest that best activities of these SPs occur at different stages of infection. The synergistic effect would allow reducing the treatment dose and toxicity and minimizing or delaying the induction of antiviral resistance; sulfated polysaccharides of the tested seaweed species thus appear as promising candidates for the development of natural antiviral agents.

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Section: Discussionmentioning

confidence: 99%

Synergistic Effects of Sulfated Polysaccharides from Mexican Seaweeds against Measles Virus

Morán-Santibañez

Cruz‐Suárez

Ricque‐Marie

et al. 2016

BioMed Research International

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“…The inhibitory effects of SSPS on viral replication in vitro have been known for more than 60 years with activities against several enveloped RNA viruses, the family of the current source of pandemic SARS-CoV-2, such as HIV (various strains), Sindbis, Semliki forest, Junin, Tacaribe, VSV, Influenza A, and RSV viruses ( Luescher-Mattli, 2003 ; Pujol et al, 2007 ; Witvrouw & De Clercq, 1997 ; Gerber et al, 1958 ; Chiu et al, 2012 ; Hayashi, Hamada & Hayashi, 1996 ; Lee et al, 2004 ; Besednova et al, 2016 ; Terasawa et al, 2020 ). As an example, SSPS was found to block HIV replication in cell cultures, at concentrations as low as 10 µg mL −1 and without showing any toxicity to the host cells at concentrations of up to 2.5 mg mL −1 ( Luescher-Mattli, 2003 ; Witvrouw & De Clercq, 1997 ).…”

Section: Introductionmentioning

confidence: 99%

Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay

Shefer

Robin

Chemodanov

et al. 2021

PeerJ

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Due to the global COVID-19 pandemic, there is a need to screen for novel compounds with antiviral activity against SARS-COV-2. Here we compared chemical composition and the in vitro anti- SARS-COV-2 activity of two different Ulva sp. crude ulvan extracts: one obtained by an HCl-based and another one by ammonium oxalate-based (AOx) extraction protocols. The composition of the crude extracts was analyzed and their antiviral activity was assessed in a cytopathic effect reduction assay using Vero E6 cells. We show that the extraction protocols have a significant impact on the chemical composition, anti- SARS-COV-2 activity, and cytotoxicity of these ulvan extracts. The ulvan extract based on the AOx protocol had a higher average molecular weight, higher charge, and 11.3-fold higher antiviral activity than HCl-based extract. Our results strongly suggest that further bioassay-guided investigation into bioactivity of compounds found in Ulva sp. ulvan extracts could lead to the discovery of novel anti-SARS-CoV-2 antivirals.

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“…The antiviral properties of sulfated polysaccharides are highly dependent on the presence of sulfate ester groups, where the higher the DS, the better the antiviral potency [112,113]. Sulfated polysaccharides with high average molecular weight generally display better antiviral properties than those with low average molecular weight.…”

Section: Antiviral Fucoidanmentioning

confidence: 99%

“…Low molecular weight sulfated polysaccharides can possess strong antiviral properties though, especially when the DS is high [113]. Although fucoidan exhibit antiviral properties both in vitro and in vivo, they have shown no direct (or on a few occasions very weak) virucidal activity [1,112,114].…”

Section: Antiviral Fucoidanmentioning

confidence: 99%

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Fucoidan-Mimetic Glycopolymers : Synthesis and Biomedical Applications

Tengdelius¹

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Cover: 3D models of three different fucoidan-mimetic glycopolymers Linköping Studies in Science and Technology Dissertations No. 1743 Printed by LiU-Tryck, Linköping, Sweden, 2016 Till morfar som inspirerade mig att börja och Lina och Theo som inspirerar mig att fortsätta. Mattias Tengdelius Fucoidan-Mimetic Glycopolymers -Synthesis and Biomedical Applications I AbstractThe marine polysaccharide fucoidan has demonstrated several interesting biological properties, for instance being antiviral, anticoagulant, anti-inflammatory, anticancer, and platelet activating. Many of these properties are desirable for various biomedical applications. Yet, there are few reports on fucoidan being used in such applications. The reasons for this are primarily the heterogeneity and low structural reproducibility of fucoidan.This thesis describes the synthesis of polymers with pendant saccharides bearing the key structural features of fucoidan. These glycopolymers were synthesized via different radical polymerization techniques yielding polymers of different chain lengths and dispersity. These glycopolymers showed antiviral and platelet activating properties similar to those of natural fucoidan, thus making them fucoidan-mimetic glycopolymers. However, compared to fucoidan from natural sources, the fucoidan-mimetic glycopolymers had homogeneous and reproducible structures making them suitable for biomedical applications.Further studies demonstrated that platelet activation, caused by these glycopolymers, showed dose-response curves almost identical to fucoidan. The platelet activation was induced via intracellular signaling and caused platelet surface changes similar to those of fucoidan. Fucoidan-mimetic glycopolymers can therefore be used as unique biomolecular tools for studying the molecular and cellular responses of human platelets.Fucoidan-mimetic glycopolymers generally assert their antiviral activity by blocking viral entry to host cells, thus inhibiting spreading of the viral infection but not acting virucidal, i.e. not killing the viruses. Introduction of hydrophobic groups to the polymer's chain ends improved the antiviral properties significantly and is an important step towards yielding glycopolymers with virucidal properties.The fucoidan-mimetic glycopolymers were also applied as capping agents when synthesizing gold nanoparticles. These fucoidan-mimetic glycopolymer coated gold nanoparticles showed improved colloidal stability compared to uncapped gold nanoparticles. Furthermore, the nanoparticles also demonstrated selective cytotoxicity against a human colon cancer cell line over fibroblast cells. III Populärvetenskaplig SammanfattningSedan urminnes tider har människan vänt sig till naturen för att bota sjukdomar och andra krämpor. Genom extrakt från framförallt växter har bland annat feber, inflammationer och olika infektioner behandlats långt innan läkemedelskonsten utvecklades till vad den är idag. Även när den moderna läkemedelsvetenskapen, farmakologin, började utvecklas var det naturen som var den främst...

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Antiviral activity and pathogenetic targets for seaweed sulfated polysaccharides in herpesvirus infections

Cited by 5 publications

References 74 publications

Synergistic Effects of Sulfated Polysaccharides from Mexican Seaweeds against Measles Virus

Synergistic Effects of Sulfated Polysaccharides from Mexican Seaweeds against Measles Virus

Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay

Fucoidan-Mimetic Glycopolymers : Synthesis and Biomedical Applications

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