Antiviral activity against enterovirus 71 of sulfated rhamnan isolated from the green alga Monostroma latissimum

Wang, Shuyao; Wang, Wei; Hao, Cui; Yu, Yunjia; Qin, Ling; He, Meijia; Mao, Wenjun

doi:10.1016/j.carbpol.2018.07.067

Cited by 49 publications

(31 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fucoidan also binds gp120 and has anti-HIV activity [27]. RS has also been reported to bind the capsid of enterovirus 71 [18]. The ionic interaction between the sulfated polysaccharide molecule and the viral glycoprotein is believed to be the main mechanism for viral inhibition; the RS derived from M. nitidum in this study may also bind viruses through a similar mechanism.…”

Section: Discussionmentioning

confidence: 64%

“…Our results showed that the oral administration of RS led to an early improvement of influenza symptoms based on the reduction of influenza virus titers in normal mice and the suppression of body weight loss in FU-treated mice. In another animal experiment, the intramuscular injection of RS derived from M. latissimum exerted a therapeutic effect on enterovirus replication [18]. However, this study is the first to report the efficacy of orally administered RS against viral infection.…”

Section: Discussionmentioning

confidence: 77%

“…The main repeating unit of RS consists of rhamnose with a sulfate-group substituent that forms long linear chains with branched side chains [10][11][12]. Several in vitro and in vivo studies have reported that RS has anticoagulative [12][13][14][15][16][17], antiviral [10,[18][19][20], anti-inflammatory [21], and anti-obesity properties [9].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae Monostroma nitidum in Mice with Normal and Compromised Immunity

et al. 2020

View full text Add to dashboard Cite

Influenza viruses cause a significant public health burden each year despite the availability of anti-influenza drugs and vaccines. Therefore, new anti-influenza virus agents are needed. Rhamnan sulfate (RS) is a sulfated polysaccharide derived from the green alga Monostroma nitidum. Here, we aimed to demonstrate the antiviral activity of RS, especially against influenza A virus (IFV) infection, in vitro and in vivo. RS showed inhibitory effects on viral proliferation of enveloped viruses in vitro. Evaluation of the anti-IFV activity of RS in vitro showed that it inhibited both virus adsorption and entry steps. The oral administration of RS in IFV-infected immunocompetent and immunocompromised mice suppressed viral proliferation in both mouse types. The oral administration of RS also had stimulatory effects on neutralizing antibody production. Fluorescent analysis showed that RS colocalized with M cells in Peyer's patches, suggesting that RS bound to the M cells and may be incorporated into the Peyer's patches, which are essential to intestinal immunity. In summary, RS inhibits influenza virus infection and promotes antibody production, suggesting that RS is a potential candidate for the treatment of influenza virus infections.

show abstract

Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae Monostroma nitidum in Mice with Normal and Compromised Immunity

et al. 2020

View full text Add to dashboard Cite

show abstract

“…EV71 infection is strongly associated with severe HFMD, which is accompanied by neurological symptoms and usually leads to death. Therefore, many studies have focused on the identification of substances with activity against EV71, and these studies have identified many candidate drugs for the treatment of EV71 infection, such as amphotericin B, isochlorogenic acid C, acid beta‐glucosidase 1, sulfated rhamnan, and suramin . Considering their relatively low cytotoxicity, extracts from traditional Chinese medicines or foods show particular advantages and have attracted increasing attention …”

Section: Discussionmentioning

confidence: 99%

Virucidal activity and the antiviral mechanism of acidic polysaccharides against Enterovirus 71 infectionin vitro

Cui

Liu

et al. 2020

Microbiology and Immunology

View full text Add to dashboard Cite

Enterovirus 71 (EV71) is the predominant pathogen for severe hand, foot, and mouth disease (HFMD) in children younger than 5 years, and currently no effective drugs are available for EV71. Thus, there is an urgent need to develop new drugs for the control of EV71 infection. In this study, LJ04 was extracted from Laminaria japonica using diethylaminoethyl cellulose‐52 with 0.4 mol/l NaCl as the eluent, and its virucidal activity was evaluated based on its cytopathic effects on a microplate. LJ04 is composed of fucose, galactose, and mannose and mainly showed good virucidal activity against EV71. The antiviral mechanisms of LJ04 were the direct inactivation of the virus, the blockage of virus binding, disruptions to viral entry, and weak inhibitory activity against the nonstructural protein 3C. The two most important findings from this study were that LJ04 inhibited EV71 proliferation in HM1900 cells, which are a human microglia cell line, and that LJ04 can directly inactivate EV71 within 2 hr at 37°C. This study demonstrates for the first time the ability of a polysaccharide from L. japonica to inhibit viral and 3C activity; importantly, the inhibition of 3C might have a minor effect on the antiviral effect of LJ04. Consequently, our results identify LJ04 as a potential drug candidate for the control of severe EV71 infection in clinical settings.

show abstract

“…Wang and coworkers found that the sulfated polysaccharide PML isolated from the green alga Monostroma latissimum (Mr ~ 513 000) significantly inhibited EV71 infection in different cells . PML blocked the viral adsorption process mainly through directly binding to viral capsid protein VP1.…”

Section: Potential Antiviral Agents Derived From Marine Glycansmentioning

confidence: 99%

Marine glycan–based antiviral agents in clinical or preclinical trials

et al. 2019

Reviews in Medical Virology

Self Cite

View full text Add to dashboard Cite

The constant outbreak of diseases caused by viral infections has caused serious harm to human health all over the world. Although many antiviral drugs have been approved for clinical use during the past decade, important issues, such as unsatisfactory efficacy, toxicity, and high cost of drugs, remain unresolved. Glycans are major components of the surfaces of both host cells and most viruses and play critical roles in the steps of viral infection. Marine glycans have more structural diversities than those found in humans. Most importantly, low toxicity and low-cost marine glycans have demonstrated potent antiviral activities through multiple molecular mechanisms. As a result, a series of marine glycan-derived agents are undergoing preclinical and clinical trials. This review discusses the recent progress in research on the marine glycan-based antiviral agents in clinical trials, relating to their structural features and clinical applications. In addition, molecular mechanisms of marine glycans involved in viral infection and novel strategies used in glycan-based drug development are critically reviewed and discussed. KEYWORDS antiviral activities, clinical trials, heparinoids, marine glycans, virus entry 1 | INTRODUCTION During the past decade, the number of antiviral drugs approved for clinical use has increased from five to more than 30, 1 but major issues with efficacy, toxicity, and cost remain unresolved. 2 Hence, the development of better antiviral agents that can address these antiviral drug issues is of high importance. Glycans are major components of the surface of both host cells and most pathogens such as bacteria and viruses. It is well established that most interactions of microbial pathogens with their hosts are influenced by the pattern of their glycans and glycan-binding receptors. 3 Glycans such as negatively charged heparins can interfere with the virus entry process through masking the positive charge of the pathogen surface receptors to prevent them from binding to host cell surface heparan sulfate proteoglycans. 4 Marine glycans, especially those from seaweed, have many advantages as antiviral drug candidates, such as relatively low production costs, low cytotoxicity, and wide availability. 2 Thus, marine glycans are attractive candidates for developing potential antiviral agents and have attracted great academic and industrial interest.This review presents an overview of recent progress in research on the marine glycan-based antiviral agents in clinical trials, relating to their structure features and clinic applications, focusing on potential antiviral agents derived from the heparinoids. Recent developments in the roles of glycans in viral infection and novel glycan-based therapeutic strategies will also be discussed.

show abstract

Antiviral activity against enterovirus 71 of sulfated rhamnan isolated from the green alga Monostroma latissimum

Cited by 49 publications

References 35 publications

Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae Monostroma nitidum in Mice with Normal and Compromised Immunity

Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae Monostroma nitidum in Mice with Normal and Compromised Immunity

Virucidal activity and the antiviral mechanism of acidic polysaccharides against Enterovirus 71 infectionin vitro

Marine glycan–based antiviral agents in clinical or preclinical trials

Contact Info

Product

Resources

About