Antiurolithic efficacy of a phenolic rich ethyl acetate fraction of the aerial parts of Aerva lanata (Linn) Juss. ex Schult. in ethylene glycol induced urolithic rats

Abstract

Objectives The study was carried out to evaluate the in vivo antiurolithic efficaciousness of an ethyl acetate fraction of Aerva lanata (EAFAL) derived from the hydromethanolic extract of its aerial parts (HMEAL). Methods In vivo pharmacological potency of EAFAL was assessed by ethylene glycol (EG) induced urolithiasis model in male Wistar albino rats. Urine samples of the animals were analysed for physical parameters, stone promoters, inhibitors along with an evaluation of the biochemical parameters of serum and kidneys. Histopathological investigation of the kidneys was done. The fraction was further subjected to LC-MS and HPLC for its phytochemical evaluation. Key findings EAFAL demonstrated a significant antiurolithic effect by a restoration of the balance between urinary promoters and inhibitors along with an amelioration of the urinary pH. The abnormally elevated levels of serum nitrogenous substances, calcium, albumin, globulin, total protein along with altered renal calcium, oxalate and uric acid were also alleviated significantly followed by an improvement of the histopathological aberrancies. Phytochemical analysis showed evidence of phenolic components and flavonoids. Conclusions The current findings prove the beneficial role of phenolic and flavonoid rich EAFAL in ameliorating urolithiasis induced abnormalities of urine, serum and kidneys.

Antioxidant, Anti-Inflammatory, and Anti-Diabetic Activity of Phenolic Acids Fractions Obtained from Aerva lanata (L.) Juss.

“…The presence of ferulic, gallic, and ellagic acids was also reported by other researchers [10,39,40]. Recently, the presence of cinnamic and chlorogenic acids has been demonstrated too [41].…”

Plants Used in Mexican Traditional Medicine for the Management of Urolithiasis: A Review of Preclinical Evidence, Bioactive Compounds, and Molecular Mechanisms

“…Some in vitro and in vivo studies suggested that the antioxidant activity of flavonoids played an important role in the prevention of the accumulation of CaOx by the reduction in ROS levels, which promoted protection against oxidative renal damage [ 64 , 65 ]. Additionally, it has been reported that the use of flavonoids inhibited CaOx crystal deposition in the urine of animal models [ 66 , 67 , 68 ].…”

LC-ESI-MS/MS Polyphenolic Profile and In Vitro Study of Cosmetic Potential of Aerva lanata (L.) Juss. Herb Extracts

“…contains a rich composition of secondary metabolites, e.g., alkaloids, sterols, and phenolic compounds. For example, the literature provides information about the presence of some flavonoid compounds in polpala, e.g., kaempferol, myricetin, apigenin, chrysin, rutin, quercetin, and isorhamnetin [5][6][7]. However, there is still no comprehensive research on phenolic secondary metabolites in the herb of A. lanata (L.) Juss.…”