Antiurolithic activity and biotransformation of galloylquinic acids by Aspergillus alliaceus ATCC10060, Aspergillus brasiliensis ATCC 16404, and Cunninghamella elegans ATCC 10028b

Abstract

Copaifera lucens n-butanolic fraction (BF) was used as a source of galloylquinic acids, and aerobically incubated with Aspergillus alliaceus ATCC10060, Aspergillus brasiliensis ATCC 16404, and Cunninghamella elegans ATCC 10028b cultures for 60 and 120 h. Out of the three studied filamentous fungi, A. alliaceus ATCC10060 was able to degrade galloylquinic acids into one major metabolite, 3-O-methylgallic acid (M1). The product was identified by 1 H-NMR, UPLC-MS/MS and its potential effect on calcium oxalate monohydrate (COM) crystal binding to Madin-Darby canine kidney cells type I surface was studied. Renal cells pretreatment with BF and M1 for 3 h significantly decreased calcium oxalate monohydrate crystal-adherence at 50 μg/mL and 5 μM, respectively. Both M1 and BF significantly reduced surface expression of COM-binding proteins annexin A1 and heat shock protein 90, respectively as evidenced by Western blot analysis of membrane, cytosolic, and whole cell lysate fractions. The compounds also showed antioxidant activities in DPPH assay.

Novel Preclinical Study of Galloylquinic Acid Compounds from Copaifera lucens with Potent Antifungal Activity against Vaginal Candidiasis Induced in a Murine Model via Multitarget Modes of Action

“…Another study stated that C. glabrata was susceptible to the leaf extract of Camellia sinensis , which is rich in polyphenolic compounds ( 20 ). Other studies mentioned that plant polyphenol-rich extracts had potential activity against pathogenic fungi ( 21 , 22 ), consistent with the polyphenolic properties of GQAs, which are capable of reducing reactive oxygen species due to their promising antioxidant properties ( 16 , 17 ). Additionally, our tested compounds showed a wider safe window in an MTT cytotoxicity assay using Vero cells, with an IC 50 of 168.17 mg/mL.…”

“…A voucher sample was kept (RB 474303) as a reference at the Laboratory of Pharmacognosy, FCFRP, Brazil. The major galloylquinic acid compounds (GQAs) were extracted and identified spectrophotometrically in the n -butanolic fraction of C. lucens , using high-performance liquid chromatography (HPLC), based on their characteristic UV spectra according to our previously reported data ( 16 , 17 ). The compounds were prepared as a stock solution (50 mg/mL) in dimethyl sulfoxide (DMSO) and stored at −80°C until use.…”

“…Copaifera species ( Fabaceae ), such as Copaifera lucens and Copaifera langsdorffii , are common trees in Brazil possessing diverse biological activities and are mainly used in urolithiasis modulation ( 16 ). Phytochemical investigations revealed the presence of a high content of galloylquinic acid compounds (GQAs), representing the major secondary metabolites in the n -butanolic and aqueous fractions of the leaf extract, which are mainly responsible for their bioactivity.…”

Calculus-related functional protein expression in ureteral calculus-adhered polyp

“…Annexin A1 that is highly expressed in epithelial cells of polyps can bind COM crystal in the urine to the surface of calculi through its transmembrane domain. [ 18 , 19 ] Alternatively, it can bind oxalic acid and calcium ions in the urine through its target protein S100 A9, which may be realized by EF-hand motifs in the S100 A9 domain. [ 4 , 20 ] In our study, correlation analysis of the H-score of annexin A1/S100 A9 indicated a strong correlation between the expressions of the 2 proteins; this suggested a synergistic relationship between annexin A1 and S100 A9 in binding COM crystal.…”

Effects of nitrogen deficiency induced by straw decomposition after incorporation into soil on the autophagy and pathogenicity of Fusarium graminearum based on an off‐line simulation

“…The degradation of GFP‐Fgatg8 in F. graminearum could be measured by Western blot analysis (El‐Salam et al., 2019). Twenty microlitres of protein sample was loaded into the stacking gel, and the voltage was kept at 100 V. Then, the voltage was increased to 130 V when the sample ran into the separating gel.…”