Antiulcerogenic Activity of Some Sesquiterpene Lactones Isolated from Artemisia annua

Foglio, Mary Ann; Dias, Patricia Correa; Antônio, Márcia Aparecida; Possenti, Ana; Rodrigues, Rodney Alexandre Ferreira; Silva, Erica Ferreira da; Rehder, Vera Lúcia Garcia; Carvalho, João Ernesto de

doi:10.1055/s-2002-32570

Cited by 44 publications

(22 citation statements)

References 9 publications

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“…The presence of dihydro-epideoxyarteannuin B in normal roots was confi rmed by CG/MS comparison with the authentic standard (Foglio et al, 2002). Gas Chromatography analysis of the root extracts revealed a peak assigned to A with 12.76 min retention time and a molecular ion in m/z 234 identical to mass profi le as dihydro-epideoxyarteannuin B (compound A).…”

Section: Resultsmentioning

confidence: 86%

“…Dihydro-epideoxyartenuin B was identifi ed comparing their spectral data with previous studies data (Foglio et al, 2002).…”

Section: Column Chromatographymentioning

confidence: 99%

“…Foglio et al (2002) demonstrated that the artemisinin extraction by-product exhibited intense antiulcerogenic activity in ulcer models induced by indometacin and ethanol comparable to the standard drug carbenoxolone. According to the authors, artemisinin did not provide cytoprotection under the experimental models tested.…”

Section: Introductionmentioning

confidence: 99%

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Biotechnology approaches for production of antiulcerogenic dihydro-epideoxyarteannuin B isolated from Artemisia annua L.

Marques¹,

Foglio²,

Morgante³

et al. 2006

Rev. bras. farmacogn.

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RESUMO: "Ferramentas biotecnológicas para a produção de dihidro-epideoxiarteanuina B, um antiulcerogênico isolado de Artemisia annua L.". Foram desenvolvidas metodologias para o estabelecimento e cultivo de raízes de Artemisia annua L. (híbrido CPQBA 2/39 x PL5). Estas raízes foram submetidas a diferentes condições de luz e a transformação genética com Agrobacterium rhizogenes (cepas 8196 e 15834). As raízes transgênicas e não-transgênicas (normais) foram cultivadas em meios de Murashige e Skoog (1962), mantidas sobre diferentes condições de fotoperíodo e analisadas para avaliação do conteúdo do composto antiulcerogênico dehidro-epideoxiarteanuína B (composto A). A confi rmação do caráter transgênico das raízes foi obtida por Dot Blot. Os extratos dos materiais vegetais foram analisados por Cromatografi a Gasosa acoplada a um Espectômetro de Massas (CG/EM). Os cromatogramas dos extratos das raízes normais revelaram a presença de dehidro-epideoxiarteanuína B e de um outro composto (composto B). As condições fotoperiódicas de cultivo infl uenciaram na produção destes dois compostos, sendo que sobre condição de escuro contínuo, dehidro-epideoxiarteanuína B foi intensamente produzido e o composto B foi detectado em pequenas proporções, enquanto que sob fotoperíodo de 16 horas, o inverso ocorreu. A quantifi cação de dehidro-epideoxiarteanuína B por Cromatografi a Gasosa acoplada a um Detector de Ionização de Chamas (CG/FID) revelou um aumento de aproximadamente cinco vezes na produção deste composto pelas raízes normais cultivadas sobre escuro contínuo em relação às raízes cultivadas na presença de 16 horas de luz. O terpeno dehidro-epideoxiarteanuína B não estava presente nas raízes transgênicas.Unitermos: Artemisia annua, terpeno, cultura de raízes in vitro, raízes transgênicas, luz.ABSTRACT: Methodologies were developed for the establishment and cultivation of Artemisia annua L (CPQBA 2/39 x PL5 hybrid) roots submitted to light conditions and genetic transformation performed with Agrobacterium rhizogenes (15834 and 8196 strains). The transgenic and nontransgenic (normal) roots were cultured in Murashige and Skoog (1962) medium, kept under different photoperiodic conditions and analyzed for evaluation of the antiulcerogenic dihydroepideoxyarteannuin B (compound A) contents. The Dot Blot technique was used to confi rm the transgenic nature of the roots. The plants′s crude extracts were analyzed by Gas Chromatography coupled to Mass Spectrum (CG/MS). The chromatograms of the extracts taken from normal roots revealed the presence of dihydro-epideoxyarteannuin B and other compound (compound B). Photoperiods during cultivation infl uenced the production of these two compounds: under continuous darkness dihydro-epideoxyarteannuin B was intensely produced and the compound B present in small amounts, while on 16 h photoperiod, the inverse occurred. The quantifi cation of dihydro-epideoxyarteannuin B by Gas Chromatography coupled to Flame Detector Ionization (CG/ FID) revealed an approximately fi vefold increase in the prod...

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Section: Resultsmentioning

confidence: 86%

“…Dihydro-epideoxyartenuin B was identifi ed comparing their spectral data with previous studies data (Foglio et al, 2002).…”

Section: Column Chromatographymentioning

confidence: 99%

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Biotechnology approaches for production of antiulcerogenic dihydro-epideoxyarteannuin B isolated from Artemisia annua L.

Marques¹,

Foglio²,

Morgante³

et al. 2006

Rev. bras. farmacogn.

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“…The resulting powder (2000 g) was submitted to dynamics maceration with ethanol during 2 hours (this procedure was repeated three times) [8]. …”

Section: Methodsmentioning

confidence: 99%

Artemisia annua L.: evidence of sesquiterpene lactones’ fraction antinociceptive activity

Favero

Grando

Nonato

et al. 2014

BMC Complement Altern Med

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BackgroundArtemisia annua L. has been used for many centuries in Chinese traditional medicine. Artemisinin, the active principle was first isolated and identified in the 1970s becoming the global back bone to the fight against malaria. Our research group previously developed an economic and ecological friendly process to obtain this compound. In the pursuit to also exploit the residue generated throughout the process we further evaluated the pharmacological potential of that extract.MethodsThe alcoholic crude extract after artemisinin precipitation maintained an enriched sesquiterpene lactones content with residue artemisinin (1.72%) and deoxyartemisinin (0.31%), used as chemical markers for this sample. This study evaluated the pharmacological potential of the enriched sesquiterpene lactone fraction (Lac-FR) on different nociceptive and inflammatory experimental animal models. Previous findings on the biological properties of lactones obtained from natural products permitted us to explore the antinociceptive activities of these compounds based on in vivo chemical-induced behavioral assays.ResultsThe enriched sesquiterpene lactone fraction (Lac-FR) was administrated by intraperitoneal injection producing a relevant reduction in the reaction time of the animals in both phases of the formalin test, significantly reduced the sensitivity to mechanical allodynia stimulus, reduced the paw edema caused by carrageenan injection and promoted high antinociceptive activity in tail flick model suggesting relationship with the opioid system. Further studies are being undertaken to elucidate the active components involved with the antinociceptive activity as well as evaluation of synergy effect that is seen by combination of substances that is greater than would be expected from consideration of individual contributions.ConclusionFor the first time, results presented herein provided consistent data to support the potential use of these lactones for pain relief as revealed by chemical-induced nociception assays in mice.

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“…O mecanismo de ação, aspectos clínicos e toxicidade da artemisinina têm sido extensamente estudados nos últimos anos, assim como estudos agrotecnológicos, farmacológicos e químicos (elucidação estrutural) de outros compostos sesquiterpênicos presentes na planta 6,11,[15][16][17][18][19][20] . Este trabalho teve como objetivo a redução dos custos de produção de artemisinina através da otimização do método de extração e purificação com o emprego de reagentes de menor custo, aliado a técnicas operacionais de execução rápida e simples.…”

Section: Figura 1 Estruturas Químicas Da Cloroquina (1) Mefloquina unclassified

Otimização do processo de extração e isolamento do antimalárico artemisinina a partir de Artemisia annua L.

Rodrigues¹,

Foglio²,

Júnior³

et al. 2006

Quím. Nova

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Antiulcerogenic Activity of Some Sesquiterpene Lactones Isolated from Artemisia annua

Cited by 44 publications

References 9 publications

Biotechnology approaches for production of antiulcerogenic dihydro-epideoxyarteannuin B isolated from Artemisia annua L.

Biotechnology approaches for production of antiulcerogenic dihydro-epideoxyarteannuin B isolated from Artemisia annua L.

Artemisia annua L.: evidence of sesquiterpene lactones’ fraction antinociceptive activity

Otimização do processo de extração e isolamento do antimalárico artemisinina a partir de Artemisia annua L.

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