Antitumoural hydroxyapatite nanoparticles‐mediated hepatoma‐targeted trans‐arterial embolization gene therapy: in vitro and in vivo studies

Abstract

In all, this new treatment based on nHAP can enhance therapeutic effect of HCC safely both in vitro and in vivo.

The cooperative effect of p53 and Rb in local nanotherapy in a rabbit VX2 model of hepatocellular carcinoma

“…11 Thus, our first aim was to enhance the effectiveness of LNT treatment through a cooperative gene effect and out second was to determine the possible molecular mechanism of this enhancement. The therapeutic protocol was designed using p53+Rb, with the aim of enhancing the antitumor effects obtained with p53 alone in the former LNT for VX2 tumors.…”

“…The event used as an endpoint was death. A P-value of #0.05 was considered For target gene expression, a previous study 11 identified the successful gene transduction only in tumor cells by observing the green fluorescence and immunostained band of EGFPp53 fusion protein using a fluorescent microscope and Western blot, respectively. This work also detected the specific expression of EGFP-p53 in nanoplex-p53/ Lipiodol (Group C) and nanoplex-(p53+Rb)/Lipiodol (Group E) group.…”

“…Immediately after the surface modification, the polyplex was prepared by the addition of pDNA into the nanoplex solution at weight ratio of 15 (previously determined as being the best weight ratio by Li et al 11 ) and left at 25°C for 30 minutes. Third, the Lipiodol ® (Guerbet, Villepinte, France)/ nanoplex and Lipiodol/polyplex emulsions were prepared by emulsifying 1 mL Lipiodol and 1 mL (3.75 mg) of the nanoplex and polyplex, respectively, according to the pumping method.…”

“…6,7 Of these, combined therapy has proven the best solution to date. [8][9][10] A previous study 11 successfully combined p53 gene therapy, embolic therapy, and nanotherapy at the liver tumor site by exploiting poly-L-lysine (PLL)-modified hydroxyapatite nanoparticles (nHAPs) to serve as embolic material and gene vector at the same time. However, complete tumor elimination was not observed in any of the animals and the survival prolongation was limited, thus further improvements to the therapy are necessary before clinical application.…”

Cytotoxicity of gold nanoclusters in human liver cancer cells

“…Li et al 21 reported that polypolex inhibited the growth of HepG2 cells and induced apoptosis, while keeping safe to L02 cell line at the same concentration. The authors deemed the different result of cytotoxicity was due to the different swollen ability between the tumor and normal cells.…”