Antitumour activity of Annona muricata L. leaf methanol extracts against Ehrlich Ascites Carcinoma and Dalton’s Lymphoma Ascites mediated tumours in Swiss albino mice

Abstract

The use of plants as a source of sedative or treatment for cancer is reasonably widespread worldwide. Annona muricata Linn exhibits a vast array of medicinal and ethno-pharmaceutical benefits, attributed by different plant parts. The activity of this plant is regarded to the bio-production of secondary metabolites like alkaloids, phenols, flavonoids, and most unique group of compounds, namely, annonaceous acetogenins. Whilst this plant is gaining popularity as an anticancer treating plant, this study was undertaken to verify the plausible anticancer effect of leaf methanol extracts of A. muricata (LEAM). Acute toxicity study was carried to obtain safe dose in mice models using haematological, biochemical, and histological evaluations in Swiss albino mice. In-vitro cytotoxicity towards Dalton's Lymphoma Ascites (DLA) and Ehrlich Ascites Carcinoma (EAC) cell lines were determined by trypan blue exclusion method. In-vivo antitumour activity of LEAM (100, 200, and 500mg/kg b.wt.) was evaluated using DLA induced solid carcinoma and EAC induced ascites carcinoma models and its comparison with standard drug Cisplatin. Acute toxicity studies did not exhibit significant variations in treated mice suggesting diminutive side effects of LEAM. Statistical analysis revealed the IC 50 values for DLA and EAC cell lines as 85.56 ± 5.28 and 68.07 ± 7.39 µg/mL, respectively, indicating better cytotoxic activity against EAC than DLA cells. LEAM decreased the tumour burden in dose-dependent manner. In comparison, with different concentrations tested, treatment with LEAM (200 mg/kg b.wt. and 500 mg/kg b.wt.) significantly reduced the solid tumour volume development by 58.11% and 65.70%, respectively. While lifespan was prolonged up to 51.43% in 500 mg/kg b.wt. LEAM treated ascites tumour-induced mice. This study thus indicates that LEAM possesses potent cytotoxic and antineoplastic activity and calls for more methodical safety assessments and other end-points of anti-tumourigenesis.

Root Bark Extract of Oroxylum indicum Vent. Inhibits Solid and Ascites Tumors and Prevents the Development of DMBA-Induced Skin Papilloma Formation

“…Dalton’s lymphoma spontaneously originates in the thymus of murine animals in the transplantable form [ 45 , 46 , 47 ] and, hence, widely used in cancer research, with tumor volume measurement as a reproducible parameter. The current study also revealed that oral administration of OIM extract brought about a gradual, but significant reduction in tumor volume, which was evident from the 12th day of induction onwards up to day 30, signifying the anticancer property of the extract, consistent with previous anticancer studies of plant extracts [ 48 , 49 ] using DLA induced tumors.…”

Medicinal plants used in cancer treatment: A survey conducted among traditional Ayurveda medical practitioners in Sri Lanka

“…Further the bark of F. leucopyrus has been identified to be effective against ovarian carcinoma (Hettihewa et al, 2015) Annona muricata was the second most commonest plant (fruits, leaves and whole plant) used for cancer therapy in this study group. Other studies have shown that leaves of Annona muricata have potent anticancer activity (Naik et al, 2021) and also has been used in breast cancers (Gavamukulya et al, 2014). The most important phychemicals of A. muricata are alkaloids, phenols and acetogenins (Coria-Téllez et al, 2018).…”

International Journal of KIU

“…Further the bark of F. leucopyrus has been identified to be effective against ovarian carcinoma (Hettihewa et al, 2015) Annona muricata was the second most commonest plant (fruits, leaves and whole plant) used for cancer therapy in this study group. Other studies have shown that leaves of Annona muricata have potent anticancer activity (Naik et al, 2021) and also has been used in breast cancers (Gavamukulya et al, 2014). The most important phychemicals of A. muricata are alkaloids, phenols and acetogenins (Coria-Téllez et al, 2018).…”