Antitumoral Effects of D-Fraction from<i>Grifola Frondosa</i>(Maitake) Mushroom in Breast Cancer

Alonso, Ernesto; Ferronato, María Julia; Gandini, Norberto Ariel; Fermento, María Eugenia; Obiol, Diego Javier; Romero, Alejandro; Arévalo, Julián; Villegas, María Emilia; Facchinetti, María Marta; Curino, Alejandro Carlos

doi:10.1080/01635581.2017.1247891

Cited by 26 publications

(23 citation statements)

References 64 publications

(82 reference statements)

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“…The present data show that Maitake D-Fraction decreases the viability of TNBC MDA-MB-231 cells through apoptosis induction. In our previous works we have demonstrated that D-Fraction also decreases the viability of hormone-dependent MCF7 [ 24 ] and hormone-independent LM3 breast cancer cells [ 25 ]. These results indicate that Maitake D-Fraction affects breast cancer cell viability regardless of hormone receptors and HER2 status of tumor cells.…”

Section: Discussionmentioning

confidence: 99%

“…More recently, increasing evidence shows that D-Fraction also exhibits immune-independent antitumoral effects on some types of cancer. Thus, the proteoglucan has antiproliferative or cytotoxic effect on PC3 (prostate cancer) [ 22 ], T24 (bladder cancer), HepG2 (liver cancer), U89 (brain cancer), HL60 (leukemia) [ 23 ], MCF7 and LM3 (breast cancer) [ 23 – 25 ], ACHN cells (kidney cancer) [ 26 , 27 ] and three canine cancer cell lines, but it does not affect the viability of A549 (lung cancer) and AGS cells (gastric cancer) [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

“…Particularly with regard to breast cancer, we have demonstrated that Maitake D-Fraction decreases the viability of hormone-dependent MCF7 cells [ 24 ] and modulates the expression of genes associated with cell proliferation, cell death, migration, invasion and metastasis among others [ 29 ]. Moreover, considering that breast cancer is a heterogeneous disease constituted by a broad spectrum of tumor subtypes, we demonstrated that D-Fraction decreases the viability and metastatic potential of hormone-independent LM3 cells in culture and reduces the tumor burden and the number of lung metastases in the LM3 syngeneic murine model [ 25 ]. However, it remains unknown whether Maitake D-Fraction has antitumoral effects in TNBC, an aggressive tumor subtype that have a limited number of treatment choices.…”

Section: Introductionmentioning

confidence: 99%

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Antitumoral and antimetastatic activity of Maitake D-Fraction in triple-negative breast cancer cells

et al. 2018

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Triple-negative breast cancer (TNBC) is associated with poor prognosis, high local recurrence rate and high rate of metastasis compared with other breast cancer subtypes. In addition, TNBC lacks a targeted therapy. This scenario highlights the need for novel compounds with high potential for TNBC treatment. In this regard, natural products are important sources of anticancer drugs. D-Fraction, a proteoglucan extracted from the edible and medicinal mushroom Grifola frondosa (Maitake), is a dietary supplement that has been shown to exert both immunostimulatory and immune-independent antitumoral effects on some cancer types. However, its antitumoral potential in TNBC is unknown. Therefore, we employed TNBC cells to investigate if D-Fraction is able to attenuate their aggressive phenotype. We found that D-Fraction decreases MDA-MB-231 cell viability through apoptosis induction and reduces their metastatic potential. D-Fraction increases cell-cell adhesion by increasing E-cadherin protein levels and β-catenin membrane localization, and increases cell-substrate adhesion. D-Fraction also decreases cell motility by affecting actin cytoskeleton rearrangements, and proteolytic activity of MMP-2 and MMP-9. Furthermore, D-Fraction decreases the invasive capacity of MDA-MB-231 cells. In concordance, D-Fraction retards tumor growth and reduces lung metastases in a xenograft model. Altogether, these results suggest the potential therapeutic role of D-Fraction in aggressive TNBC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antitumoral and antimetastatic activity of Maitake D-Fraction in triple-negative breast cancer cells

et al. 2018

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“…Grifola frondosa is a species of maitake and its d -fraction is rich in proteoglucan, which has long been exclusively attributed to their immune-stimulatory capacity. In particular, it decreases cell viability, increases cell adhesion, and reduces the migration and invasion of mammary tumor cells, generating a less aggressive cell behavior in a murine model of breast cancer [ 34 ]. G. frondosa also inhibits hepatocellular carcinoma by inhibiting proliferation, inducing cell cycle arrest, and inducing apoptosis in Hep3B hepatoma cells [ 35 ].…”

Section: Medicinal Properties Of Mushroommentioning

confidence: 99%

A Critical Review on Health Promoting Benefits of Edible Mushrooms through Gut Microbiota

Jayachandran

Xiao

2017

IJMS

196

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Mushrooms have long been used for medicinal and food purposes for over a thousand years, but a complete elucidation of the health-promoting properties of mushrooms through regulating gut microbiota has not yet been fully exploited. Mushrooms comprise a vast, and yet largely untapped, source of powerful new pharmaceutical substances. Mushrooms have been used in health care for treating simple and common diseases, like skin diseases and pandemic diseases like AIDS. This review is aimed at accumulating the health-promoting benefits of edible mushrooms through gut microbiota. Mushrooms are proven to possess anti-allergic, anti-cholesterol, anti-tumor, and anti-cancer properties. Mushrooms are rich in carbohydrates, like chitin, hemicellulose, β and α-glucans, mannans, xylans, and galactans, which make them the right choice for prebiotics. Mushrooms act as a prebiotics to stimulate the growth of gut microbiota, conferring health benefits to the host. In the present review, we have summarized the beneficial activities of various mushrooms on gut microbiota via the inhibition of exogenous pathogens and, thus, improving the host health.

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“…Diet appears to be one of the ways by which carcinogenic agent instigate the https://doi.org/10.1016/j.fct.2018.01.015 Received 4 December 2017; Received in revised form 9 January 2018; Accepted 10 January 2018 DNA repair modulating the intensity of early carcinogenic events (Rejhová et al, 2017). Several natural chemopreventive food compounds are being evaluated as edible and medicinal supplements, including the ones found in mushrooms, with antitumor substances already been identified in many species (Alonso et al, 2017;Gogavekar et al, 2014;Jin et al, 2016;Santesso and Wieland, 2016). Antiproliferative activity of Pleurotus sajor-caju (Fr) Singer extracts against human tumor cell lines (Hep-2, HeLa, Sarcoma 180, among others) has been reported (Assis et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis induction by Pleurotus sajor-caju (Fr.) Singer extracts on colorectal cancer cell lines

Finimundy

Abreu

Bonetto

et al. 2018

Food and Chemical Toxicology

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Pleurotus sajor-caju (PSC) is an edible mushroom used in food supplements, presenting antitumor properties through induction of cell death pathways. The PSC potential against colorectal cancer was analyzed by exposing HCT116 cells to different PSC extracts. The PSC n-hexane extract (PSC-hex) showed the highest cytotoxicity effect (IC value 0.05 mg/mL). The observed cytotoxicity was then associated to apoptosis-promoting and cell cycle-arrest pathways. PSC-hex was able to induce apoptosis related to breakdown of mitochondrial membrane potential and ROS generation. The absence of cytotoxicity in HTC116 and HTC116 cells, alongside with an increase in p53, Bax and Caspase-3 expression, and decrease in Bcl-2 expression, supports that the pro-apoptotic effect is probably induced through a p53 associated pathway. PSC-hex induced cell cycle arrest at G2/M in HCT116 without cytotoxicity in HTC116 cells. These findings suggest that a p21/p53 cell cycle regulation pathway is probably disrupted by compounds present on PSC-hex. Identification of the major components was then performed with ergosta-5,7,22-trien-3β-ol representing 30.6% of total weight. In silico docking studies of ergosta-5,7,22-trien-3β against Bcl-2 were performed and results show a credible interaction with the Bcl-2 hydrophobic cleft. The results show that PSC-hex can be used as supplementary food for adjuvant therapy in colorectal carcinoma.

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Antitumoral Effects of D-Fraction fromGrifola Frondosa(Maitake) Mushroom in Breast Cancer

Cited by 26 publications

References 64 publications

Antitumoral and antimetastatic activity of Maitake D-Fraction in triple-negative breast cancer cells

Antitumoral and antimetastatic activity of Maitake D-Fraction in triple-negative breast cancer cells

A Critical Review on Health Promoting Benefits of Edible Mushrooms through Gut Microbiota

Apoptosis induction by Pleurotus sajor-caju (Fr.) Singer extracts on colorectal cancer cell lines

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