Antitumoral activily in an organic extract of the sea anemone Bunodosoma caissarum

Malpezzi, E L A; Matsui, Daigo; Groote, S.C.T.S.; Freitas, J.C.; Santelli, Giovanni; Fernandas, J.B.

doi:10.1016/0041-0101(95)99327-y

Cited by 6 publications

(3 citation statements)

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“…Consistent with this observation, there was significantly diminished viability of A549 cells by H. crispa in the current study. Malpezzi et al (26) reported that Bunodosoma caissarum, which presents antimitotic activity, was fractionated by partition and adsorption on silica gel to identify the active substances. N-butanol from lipid fractions was tested on T47D cells at concentrations ranging from 50 to 500 μg/mL.…”

Section: Discussionmentioning

confidence: 99%

Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms

Ramezanpour¹,

Silva²,

Sanderson³

2012

J. Venom. Anim. Toxins incl. Trop. Dis

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Although sea anemones are well known for being rich sources of toxins, including cytolysins and neurotoxins, their venoms and toxins have been poorly studied. In the present study, the venoms from five sea anemones (Heteractis crispa, Heteractis magnifica, Heteractis malu, Cryptodendrum adhaesivum and Entacmaea quadricolor) were obtained by the milking technique, and the potential of these venoms to kill cancer cells was tested on three cell lines (A549 lung cancer, T47D breast cancer and A431 skin cancer). The total protein level in the crude extract was determined by the bicinchoninic acid (BCA) protein assay. The cytotoxicity on different cell lines was assayed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay which measures survival based on the detection of mitochondrial activity and by the crystal violet assay, which measures survival based on the ability of cells to remain adherent to microplates. The results indicate that the sea anemone venom is cytotoxic to human cancer cells. The A549 cell line was the most sensitive of the cell lines tested with a significant reduction in viability observed at 40 µg/mL. H. malu, C. adhaesivum and E. quadricolor had a significant inhibitory effect on A431 cells. Furthermore, H. malu and C. adhaesivum had a significant inhibitory effect on T47D cell line at 40 µg/mL. In conclusion, the sea anemone venoms tested have the potential to be developed as anticancer agents.

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Section: Discussionmentioning

confidence: 99%

Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms

Ramezanpour¹,

Silva²,

Sanderson³

2012

J. Venom. Anim. Toxins incl. Trop. Dis

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“…No Brasil existem alguns antecedentes de estudos químicos em cnidários, principalmente anêmonas e octocorais. Freitas e colaboradores trabalham desde a década do 80 com substancias biactivas de anêmonas-do-mar, principalmente com Bunodosoma caissarum e B. cangicum, encontrando atividade antimitótica, hemolítica, neurotóxica e antitumoral em câncer de mama humano Elena, 1991;Lagos et al, 2001;Malpezzi et al, 1995;Oliveira et al, 2004).…”

Section: Introductionunclassified

Avaliação da atividade antiinflamatória e analgésica do extrato bruto hidroalcoólico do zoantídeo Palythoa caribaeorum (Duchassaing & Michelotti, 1860)

Soares

Pérez

Maia

et al. 2006

Rev. bras. farmacogn.

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RESUMO:O presente trabalho representa a primeira análise das atividades analgésica e antiinfl amatória do extrato bruto hidroalcoólico do zoantídeo Palythoa caribaeorum. Foram realizados os testes de Writhing induzido por ácido acético (1%, 10 mL.kg -1 i.p.) e da chapa quente em camundongos para avaliar o efeito analgésico; e o teste de edema de pata de rata induzido por carragenina para avaliar a atividade antiinfl amatória. As análises evidenciaram atividade analgésica no teste de Writhing no grupo tratado com 200 mg.kg -1 v.o. de extrato, com uma inibição de 47,22% do número de contorções abdominais ("Writhings"), revelando atividade estatisticamente signifi cativa. INTRODUÇÃOOs cnidários antozoários são um grupo de organismos coloniais ou solitários, que apresentam uma grande variedade de formas, tamanhos e cores, habitando todos os mares do mundo, desde zonas costeiras até abissais (Pérez, 1999). Os zoantídeos são cnidários polipóides exclusivamente marinhos e habitantes dos recifes costeiros. Palythoa é um gênero de zoantídeos coloniais cujas espécies se caracterizam pela incorporação de grão de sedimento nos tecidos da sua parede corporal. Na espécie Palythoa caribaeorum (Duchassaing & Michelotti, 1860) os pólipos estão conectados por um espesso tecido chamado cenénquima, o qual também agrega partículas na sua superfície. Estas colônias formam extensos "tapetes" localizados nas áreas submersas, mas quando estão expostos durante a maré baixa, seus pólipos produzem um muco que protege a colônia da dessecação. O muco da o nome popular de "baba-de-boi". Este zoantídeo é o mais abundante do litoral pernambucano, ocupando extensas regiões sobre os recifes.O estudo químico com cnidários teve grande impulso durante a década do 60 com o descobrimento das prostaglandinas em varias espécies de corais moles do Caribe (em especial a gorgónia Plexaura homomalla) (Weinheimer;Spraggins, 1969). A extrema diversidade dos efeitos biológicos das prostaglandinas provocou, naquele momento, a coleta indiscriminada destas espécies, pondo em perigo as populações das mesmas em varias regiões caribenhas. Felizmente o desenvolvimento de métodos de síntese em laboratório avançou rapidamente, resguardando estes corais (Correia et al., 2002). Desde então, fi cou em evidencia o potencial destes organismos como produtores de compostos de interesse biológico e farmacológico. A maior quantidade de metabólitos secundários de cnidários foi isolada de octocorais (alcionáceos e gorgonáceos) e de zoantídeos (Rodríguez;Cobar, 1993;Babu et al., 1997). Existem muitos exemplos de terpenoides de corais que possuem atividade biológica ou farmacológica. Rinehart et al. (1981) estudaram a atividade antiviral, antimicrobiana e

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“…Bunodosoma caissarum ( B. caissarum ) is a Brazilian sea anemone species that populates the intertidal zone of the southern coast. In the 1990s, B. caissarum ’s extract was thoroughly studied by Malpezzi and Freitas research group, which published several findings related to its antimitotic, antitumor, cytotoxic, hemolytic, and neurotoxic activities [ 15 , 16 , 17 , 18 ], and described the first three toxins of this species [ 19 ]. However, access to different databases shows an absence of genome annotation for the genus of B. caissarum , and the number of deposited genomes for the phylum Cnidaria is still relatively low.…”

Section: Introductionmentioning

confidence: 99%

Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum

et al. 2023

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Sea anemones are sessile invertebrates of the phylum Cnidaria and their survival and evolutive success are highly related to the ability to produce and quickly inoculate venom, with the presence of potent toxins. In this study, a multi-omics approach was applied to characterize the protein composition of the tentacles and mucus of Bunodosoma caissarum, a species of sea anemone from the Brazilian coast. The tentacles transcriptome resulted in 23,444 annotated genes, of which 1% showed similarity with toxins or proteins related to toxin activity. In the proteome analysis, 430 polypeptides were consistently identified: 316 of them were more abundant in the tentacles while 114 were enriched in the mucus. Tentacle proteins were mostly enzymes, followed by DNA- and RNA-associated proteins, while in the mucus most proteins were toxins. In addition, peptidomics allowed the identification of large and small fragments of mature toxins, neuropeptides, and intracellular peptides. In conclusion, integrated omics identified previously unknown or uncharacterized genes in addition to 23 toxin-like proteins of therapeutic potential, improving the understanding of tentacle and mucus composition of sea anemones.

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Antitumoral activily in an organic extract of the sea anemone Bunodosoma caissarum

Cited by 6 publications

References 0 publications

Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms

Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms

Avaliação da atividade antiinflamatória e analgésica do extrato bruto hidroalcoólico do zoantídeo Palythoa caribaeorum (Duchassaing & Michelotti, 1860)

Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum

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