Antitumor potential of SLPI promoter controlled recombinant caspase-3 expression in laryngeal carcinoma

Chen, J; Yang, Baoyu; Zhang, S; Ling, Yi He; Ye, J; Jia, Zhenyu; Cao, Jiashu

doi:10.1038/cgt.2012.5

Cited by 14 publications

(20 citation statements)

References 32 publications

(20 reference statements)

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“…18,19 This protein may be a inhibitor of CASP3 and CASP7. 20 In light of the previous reports, however, the effect of miR-203 and survivin on the proliferation of HCC cells, as well as the functional relationship between miR-203 and survivin in HCC cells has not been documented.…”

Section: Discussionmentioning

confidence: 93%

miR‐203 inhibits proliferation of HCC cells by targeting survivin

Wang

Liu²,

Sun

et al. 2012

Cell Biochemistry & Function

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To validate whether down-regulation of microRNA-203 (miR-203) in hepatocellular carcinoma (HCC) is involved in HCC progression by targeting survivin. MiR-203 mimics was transfected into HepG2 cells to enhance miR-203 expression, and miR-203 inhibitor was transfected into HepG2 cells to inhibit miR-203 expression. The effect of up-regulation and down-regulation of miR-203 on survivin expression of HepG2 cells was evaluated using Western blot assay. The effect of miR-203 or survivin expression on the proliferation of HepG2 cells was detected using the CKK-8 assay. Over-expression of miR-203 significantly inhibited the expression of survivin in HepG2 cells (p < 0·05), and down-expression of miR-203 significantly promoted the expression of survivin in HepG2 cells (p < 0·05). Both over-expression of miR-203 and down-regulation of survivin suppressed proliferation of HepG2 cells significantly compared with negative control. Low expression of miR-203 contributes to the progression of HCC via targeting survivin.

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Section: Discussionmentioning

confidence: 93%

miR‐203 inhibits proliferation of HCC cells by targeting survivin

Wang

Liu²,

Sun

et al. 2012

Cell Biochemistry & Function

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“…Despite the LSCC treatment strategies that have been developed in the past two decades, 32 , 33 , 34 satisfactory therapeutic outcomes and survival rates of patients with LSCC have not improved significantly. 35 Drug resistance and recurrence of malignancy are responsible for the uncertain therapeutic effects.…”

Section: Discussionmentioning

confidence: 99%

Contribution of TIP30 to chemoresistance in laryngeal carcinoma

et al. 2014

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Laryngeal squamous cell carcinoma (LSCC) is one of the most common carcinomas of the head and neck. Despite advances in diagnosis and treatment, the survival of patients with LSCC has not improved in the past two decades. TIP30, a newly identified tumour suppressor, appears to be involved in multiple processes during tumour development. Here, we investigated the involvement of TIP30 in chemoresistance of LSCC in vitro and in vivo. We showed that TIP30 expression decreased significantly in drug-selected cells (DSCs) of laryngeal carcinoma. Suppressing TIP30 enhanced resistance capability to multiple chemotherapy drugs, cell proliferation and self-renewal in Hep2 cells. Additionally, decreased self-renewal capacity and chemotherapeutic resistance were observed in DSCs overexpressing TIP30. Furthermore, TIP30 negatively regulated tumourigenesis and chemoresistance in LSCC cells subcutaneously transplanted into nude mice. Moreover, decreased TIP30 expression contributed to chemoresistance, self-renewal and proliferation of LSCC cells via nuclearlisation of β-catenin, a cell–cell adhesion and stem cell renewal regulator. Consistently, Kaplan–Meier and Cox proportional hazards regression modelling analyses showed that decreased TIP30 expression independently predicted poor survival in patients with LSCC. Taken together, our results reveal that TIP30 has a crucial role in chemoresistance of LSCC through the AKT/glycogen synthase kinase-3β/β-catenin signalling pathway and may be a promising candidate for improving LSCC chemotherapy.

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“…Several state-of-the-art treatment strategies have been developed for LSCC, including molecular targeted therapy [18], gene therapy [19] and immunotherapy [20]. However, no treatment could achieve satisfactory therapeutic outcome and the survival rate of LSCC has not been improved significantly [21].…”

Section: Discussionmentioning

confidence: 99%

Alpha B-crystallin is a new prognostic marker for laryngeal squamous cell carcinoma

Yuan

Zhang

Lin

et al. 2012

J Exp Clin Cancer Res

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BackgroundAlpha B-crystallin (αB-crystallin) has been suggested to play an important role in the development of solid tumors. However, the association between αB-crystallin expression and clinicopathological characteristics of human laryngeal carcinoma is not well defined. This study aimed to examine the expression of αB-crystallin in human laryngeal squamous cell carcinoma (LSCC) and investigate the relationship between its expression and the prognosis of LSCC.MethodsReal-time polymerase chain reaction (six LSCC samples, six tumor-adjacent normal samples) and immunohistochemistry by tissue microarrays (109 LSCC samples and 28 tumor-adjacent normal samples) were performed to characterize expression of the αB-crystallin gene in LSCC. Kaplan-Meier survival and Cox regression analyses were carried out to evaluate the prognosis of LSCC.ResultsReal-time polymerase chain reaction and immunohistochemistry analysis showed that the expression of αB-crystallin in LSCC was significantly higher than that in tumor-adjacent normal tissues. Moreover, the expression level of αB-crystallin protein in LSCC was significantly related to alcohol consumption (P = 0.022), tumor differentiation (P = 0.007), pTNM stage (P = 0.041) and 5 years’ survival (P =0.030). COX multi-factor analysis showed that αB-crystallin (P = 0.013), as well as pTNM stage (P =0.027) and lymphatic metastasis (P = 0.015) were independent prognosis factors for LSCC.ConclusionsThe data suggest that αB-crystallin expression is correlated with malignant phenotypes of LSCC and it may serve as a novel prognostic factor for LSCC.

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Antitumor potential of SLPI promoter controlled recombinant caspase-3 expression in laryngeal carcinoma

Cited by 14 publications

References 32 publications

miR‐203 inhibits proliferation of HCC cells by targeting survivin

miR‐203 inhibits proliferation of HCC cells by targeting survivin

Contribution of TIP30 to chemoresistance in laryngeal carcinoma

Alpha B-crystallin is a new prognostic marker for laryngeal squamous cell carcinoma

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