Antitumor potential of Pllans–II, an acidic Asp49–PLA2 from Porthidium lansbergii lansbergii snake venom on human cervical carcinoma HeLa cells

Jiménez-Charris, Eliécer; Lopes, Daiana Silva; Gimenes, Sarah Natalie Cirilo; Teixeira, Samuel Cota; Montealegre–Sánchez, Leonel; Solano–Redondo, Luis; Fierro–Pérez, Leonardo; Ávila, Veridiana de Melo Rodrigues

doi:10.1016/j.ijbiomac.2018.09.053

Cited by 25 publications

(37 citation statements)

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“…The presence of active sites is essential in PLA 2 catalytic action, with this activity dependent on calcium ions as a cofactor [62,63]. Function prediction using PROSITE indicates that A2-EPTX-Nsm1a is an Asp49 PLA2, a type of PLA 2 commonly found in snake venoms [10,27,64]. This aspartic active site is vital for the snake venom PLA 2 catalytic network with His48, Tyr52, and Tyr64 residues [65].…”

Section: Discussionmentioning

confidence: 99%

“…PLA 2 toxins isolated from elapid and viperid snake venoms have been reported to cause muscle necrosis [8][9][10][11][12][13][14][15][16], induction of inflammatory cytokines [9,12,15,[17][18][19][20][21], neurotoxicity [15,[22][23][24][25], edema [9,14,18,19,21], hypotension [26], vasoconstriction [27], hemolysis [28], pulmonary congestion [12], intraperitoneal bleeding [12], and acute kidney injury [29]. Some snake venom PLA 2 s have been reported to have potential therapeutic activities including anti-cancer [30][31][32][33][34][35], anti-angiogenic [36], antibacterial [37,38], antiparasite [39], antithrombotic [40], anticoagulant [8,12,28,…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A2 from Malaysian Spitting Cobra (Naja sumatrana) Venom

Abdullah

Rusmili

Abidin

et al. 2021

Toxins

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Phospholipase A2 (PLA2) toxins are one of the main toxin families found in snake venom. PLA2 toxins are associated with various detrimental effects, including neurotoxicity, myotoxicity, hemostatic disturbances, nephrotoxicity, edema, and inflammation. Although Naja sumatrana venom contains substantial quantities of PLA2 components, there is limited information on the function and activities of PLA2 toxins from the venom. In this study, a secretory PLA2 from the venom of Malaysian N. sumatrana, subsequently named A2-EPTX-Nsm1a, was isolated, purified, and characterized. A2-EPTX-Nsm1a was purified using a mass spectrometry-guided approach and multiple chromatography steps. Based on LC-MSMS, A2-EPTX-Nsm1a was found to show high sequence similarity with PLA2 from venoms of other Naja species. The PLA2 activity of A2-EPTX-Nsm1 was inhibited by 4-BPB and EDTA. A2-EPTX-Nsm1a was significantly less cytotoxic in a neuroblastoma cell line (SH-SY5Y) compared to crude venom and did not show a concentration-dependent cytotoxic activity. To our knowledge, this is the first study that characterizes and investigates the cytotoxicity of an Asp49 PLA2 isolated from Malaysian N. sumatrana venom in a human neuroblastoma cell line.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A2 from Malaysian Spitting Cobra (Naja sumatrana) Venom

Abdullah

Rusmili

Abidin

et al. 2021

Toxins

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“…Likewise, Cañas reported a case of brainstem ischemic stroke after a B. atrox snakebite, evidencing the incidence and importance of cardiovascular complications of bothropic snakebite in Colombia (105). Similarly, Jimenez-Charris et al (106)(107)(108) from Universidad del Valle as well, conducted a proteomic a study of P. lansbergii lansbergii from the department of Atlántico, where the activities of acidic and basic PLA 2 s were tested, and the antitumoral activity of a particular acidic-PLA 2 activity was evaluated. These works have enriched our knowledge about P. lansbergii lansbergii, which had not been studied before.…”

Section: Other Researchesmentioning

confidence: 91%

Toxinology in Colombia: Contributions of Programa De Ofidismo/Escorpionismo and Other Research Groups

Pereañez

Preciado

Romero

2020

Vitae

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Background: Toxinology is a field of toxicology involved in studying toxins from animals, plants and microorganisms. In Colombia, in last thirty years, this area has been mainly studied by Programa de Ofidismo/Escorpionismo of the Universidad de Antioquia, however, some other research groups have been also contributed in our knowledge of venoms, toxin, effects and treatments. Objective: highlights the most important findings in toxinology made by Programa de Ofidismo/Escorpionismo and other research groups in Colombia. Methods: we collected and analyze119 manuscripts dealing with the history of ophidiology and toxinology in Colombia. Results: we describe some useful terms to understand the toxinology and their scopes. In addition, we build a brief history of ophidiology beginning with the period corresponding to the discovery of America, and ending with recent findings. Finally, we perform a great description of several results related to toxin isolation, characterization, antivenoms, clinical trials, new species descriptions, pretoemic and transcriptomic, among other. The nineteens was characterized by the study of snakebites, their clinic manifestations and the use of antivenoms. In addition, the ethnopharmacological studies of medicinal plants used in snakebite treatments began to be explored. The 2000s included the newly ethnopharmacology, toxin isolation, clinical trials, inhibitor studies, scorpion venom characterization and scorpion stings features. Finally, from 2010 until today, proteomic and transcriptomic gave the most important findings. Conclusions: Toxinology works in Colombia have contributed in our knowledge about endemic species, clinical manifestations of snakebites and scorpion stings, however, we invite to Colciencias and other funding agencies to have more resources in order to support more researchers in this field, since, snakebite is a public neglected disease and need more attention from governments and academic people. Finally, we still have ignorance about the venoms of some species and their possible mode of action. In addition, given the complexity of venoms, we ignore the potential use of toxins in current biomedicine. Thus, we must continue performing studies in toxinology.

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“…Therefore, the cytotoxicity of sPLA 2 is probably mediated by the interaction between the C-terminal region and the plasma membrane [ 73 , 74 ]. Moreover, the PLA 2 effects can be mediated through the integrins and other receptors, such as vascular endothelial growth factor receptor-2 (VEGFR-2), and M–Type receptors [ [75] , [76] , [77] ]. Recently, it was demonstrated that the cell surface nucleolin interacts with and internalizes the PLA 2 -like Bothrops asper myotoxin-II, which is responsible to mediate its toxic activity [ 78 ].…”

Section: Phospholipases a 2 From Snake Venommentioning

confidence: 99%

Insights into the antiviral activity of phospholipases A2 (PLA2s) from snake venoms

Teixeira

Borges

Oliveira

et al. 2020

International Journal of Biological Macromolecules

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Viruses are associated with several human diseases that infect a large number of individuals, hence directly affecting global health and economy. Owing to the lack of efficient vaccines, antiviral therapy and emerging resistance strains, many viruses are considered as a potential threat to public health. Therefore, researches have been developed to identify new drug candidates for future treatments. Among them, antiviral research based on natural molecules is a promising approach. Phospholipases A 2 (PLA 2 s) isolated from snake venom have shown significant antiviral activity against some viruses such as Dengue virus, Human Immunodeficiency virus, Hepatitis C virus and Yellow fever virus, and have emerged as an attractive alternative strategy for the development of novel antiviral therapy. Thus, this review provides an overview of remarkable findings involving PLA 2 s from snake venom that possess antiviral activity, and discusses the mechanisms of action mediated by PLA 2 s against different stages of virus replication cycle. Additionally, molecular docking simulations were performed by interacting between phospholipids from Dengue virus envelope and PLA 2 s from Bothrops asper snake venom. Studies on snake venom PLA 2 s highlight the potential use of these proteins for the development of broad-spectrum antiviral drugs.

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Antitumor potential of Pllans–II, an acidic Asp49–PLA2 from Porthidium lansbergii lansbergii snake venom on human cervical carcinoma HeLa cells

Cited by 25 publications

References 50 publications

Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A2 from Malaysian Spitting Cobra (Naja sumatrana) Venom

Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A2 from Malaysian Spitting Cobra (Naja sumatrana) Venom

Toxinology in Colombia: Contributions of Programa De Ofidismo/Escorpionismo and Other Research Groups

Insights into the antiviral activity of phospholipases A2 (PLA2s) from snake venoms

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