Antitumor effects of Scutellariae radix and its components baicalein, baicalin, and wogonin on bladder cancer cell lines

Ikemoto, Shinichi; Sugimura, Kazunobu; Yoshida, Naomasa; Yasumoto, R; Wada, Seiji; Yamamoto, Keisuke; Kishimoto, Toshifumi

doi:10.1016/s0090-4295(00)00467-2

Cited by 269 publications

(177 citation statements)

References 16 publications

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“…Their results indicated that baicalein was a precursor metabolite of baicalin synthesis, and it was suggested that the synthesis of baicalein and/or wogonin would affect the accumulation of baicalin in S. baicalensis cells. In human cell lines, the proliferation of bladder cancer cells and hepatoma cells were known to be suppressed by baicalein, baicalin and wogonin (Chang et al, 2002;Ikemoto et al, 2000). Interestingly, in the present work the different effects between the cancer cell lines and normal diploid cell line were observed by the addition of baicalein, baicalin and wogonin (Himeji et al, 2007).…”

Section: The Overview On Utilization Of S Baicalensisproducing Flavosupporting

confidence: 40%

High-yield production of scutellaria radix flavonoids (baicalein, baicalin and wogonin) by liquid-culture of Scutellaria baicalensis root-derived cells

Ohtsuki

Himeji

Fukazawa

et al. 2009

Braz. arch. biol. technol.

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Section: The Overview On Utilization Of S Baicalensisproducing Flavosupporting

confidence: 40%

High-yield production of scutellaria radix flavonoids (baicalein, baicalin and wogonin) by liquid-culture of Scutellaria baicalensis root-derived cells

Ohtsuki

Himeji

Fukazawa

et al. 2009

Braz. arch. biol. technol.

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“…Flavonoids such as baicalin, wogonoside, wogonin, and oroxylin A are the main constituents in the radix of this plant, and more than 30 flavonoids have been identified up till now [3]. The extract of S. baicalensis has been reported to possess a variety of biological effects including anti-oxidant, anti-inflammatory, anti-bacterial, anti-tumor, and anti-diabetic activities [4][5][6][7][8]. Baicalin was shown to possess anti-diabetic activities in type 2 diabetic Goto-Kakizaki rats [9], and baicalin, baicalein, and wogonin were found to be α-glucosidase inhibitors, which are considered to be potential leading compounds for development of anti-diabetes drug [10].…”

Section: Introductionmentioning

confidence: 99%

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

et al. 2016

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Scutellaria baicalensis is a traditional Chinese medicinal plant possessing a wide variety of biological activities. In this work, lipase immobilized on magnetic nanoparticles (LMNPs) was used as solid phase extract absorbent for screening of lipase inhibitors from this plant. Three flavonoids were found to bind to LMNPs and were identified as baicalin, wogonin, and oroxylin A by liquid chromatography-mass spectrometry (HPLC-MS). Their IC 50 values were determined to be 229.22 ± 12.67, 153.71 ± 9.21, and 56.07 ± 4.90 μM, respectively. Fluorescence spectroscopy and molecular docking were used to probe the interactions between these flavonoids and lipase. All the flavonoids quenched the fluorescence of lipase statically by forming new complexes, implying their affinities with the enzyme. The thermodynamic analysis suggested that van der Waals force and hydrogen bond were the main forces between wogonin and lipase, while hydrophobic force was the main force for the other two flavonoids. The results from a molecular docking study further revealed that all of them could insert into the pocket of lipase binding to a couple of amino acid residues.

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“…The only growth inhibition assay that has been carried out with neobaicalein was done on the LNCaP and PC-3 cells (Bonham et al, 2005). Neobaicalein-mediated inhibition of HeLa cells growth has not been described before, although wogonin has been reported to reduce the proliferation of several cell types (Ikemoto et al, 2000;Sonoda et al, 2004) including HeLa cells (Yang et al, 2009;Yu et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Wogonin and neobaicalein from Scutellaria litwinowii roots are apoptotic for HeLa cells

Tayarani-Najarani¹,

Asili²,

Parsaee³

et al. 2012

Rev. bras. farmacogn.

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Chemical investigation on the CH 2 Cl 2 fraction of the Scutellaria litwinowii Bornm. & Sint., Lamiaceace, root extract for the fi rst time resulted in the isolation of wogonin, and neobaicalein. These compounds were evaluated for their cytotoxicity towards HeLa cell lines and lymphocytes. Meanwhile, the role of apoptosis was explored in this toxicity. The cells were cultured in RPMI medium and incubated with different concentrations of isolated fl avonoids. Cell viability was quantifi ed by MTS assay. Apoptotic cells were determined using propidium iodide staining of DNA fragmentation by fl ow cytometry (sub-G1peak). Wogonin, and neobaicalein inhibited the growth of malignant cells in a dose-dependent manner. The IC50 values of 46.62 and 79.34 µM were, respectively, found for neobaicalein and wogonin against HeLa cells after 48 h of treatment. Neobaicalein induced a sub-G1 peak in the fl ow cytometry histogram of treated cells compared to control cells indicating that apoptotic cell death is involved in neobaicalein toxicity. Neobaicalein exerts cytotoxic and pro-apoptotic effects in HeLa cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.

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Antitumor effects of Scutellariae radix and its components baicalein, baicalin, and wogonin on bladder cancer cell lines

Cited by 269 publications

References 16 publications

High-yield production of scutellaria radix flavonoids (baicalein, baicalin and wogonin) by liquid-culture of Scutellaria baicalensis root-derived cells

High-yield production of scutellaria radix flavonoids (baicalein, baicalin and wogonin) by liquid-culture of Scutellaria baicalensis root-derived cells

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

Wogonin and neobaicalein from Scutellaria litwinowii roots are apoptotic for HeLa cells

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