Antitumor effect of the conjugate of synthetic carbohydrate moiety of the tumor-associated ganglioside GM3 with hemocyanin in combination with the cytostatic cyclophosphamide

Synthesis of a conjugate of 3´-sialyllactoside with recombinant flagellin as a carrier protein and assessment of its immunological activity in comparison with that of a similar hemocyanin-based conjugate

“…29-32 A conjugate 28 synthesized by our group was administered to mice with transplanted melanoma В16, and it was found to stimulate induction of IgM and IgG antibodies specific to the carbohydrate moiety of GM3 ganglioside and suppress the tumor growth. 28 Also, the injection of dendritic cells, which were pulsed with this conjugate, induced production of antibodies and cyto toxic immune response against В16 melanoma cells, which express GM3. 33 It is known, that the efficacy of the den dritic cell based vaccine depends not only on the correct choice of the antigen, but also on the phenotype of den dritic cells, which is, in turn, determined by exogenic factors, and, among others, on the structure of the carrier protein.…”