Antitumor Effect of Culinary-Medicinal Oyster Mushroom, Pleurotus ostreatus (Jacq.: Fr.) P. Kumm., Derived Protein Fraction on Tumor-Bearing Mice Models

Abstract

Previously, we reported the in vitro anticancer and immunomodulatory effect of a protein fraction designated as Cibacron blue affinity purified protein (CBAEP) obtained from the culinary-medicinal oyster mushroom, Pleurotus ostreatus. In the present study, we investigated the in vivo antitumor potential of CBAEP in different tumor-bearing mice models and studied the detailed mechanism of tumor regression in Dalton lymphoma (DL)-bearing mice. The lethal dose (LD50) of CBAEP was found to be 55 mg/kg body weight and sublethal doses (5 mg/kg and 10 mg/kg body weight) showed a prolonged tumor survival time in DL, Sarcoma-180, and B16F0 melanoma tumor-bearing mice. Further, CBAEP reduced about 35.68 and 51.43% DL cell growth in 5 and 10 mg/kg body weight, respectively. The in vivo CBAEP treatment showed an apoptotic feature as demonstrated in morphological study and sub-G0/G1 population in cell cycle and Western blot of DL cells. CBAEP also activated immunosuppression condition in DL tumor-bearing host. It also stimulated immune cells in the presence of nonspecific immunostunulator (LPS and ConA) ex vivo as well as enhanced Th1 response with production of TNF-α, IFN-γ, and IL-2. Moreover, it activated tumor-associated macrophages and NK cells. The present findings revealed the potent antitumor property of CBAEP, which might help in developing a new anticancer drug.

Pleurotus highking Mushroom Induces Apoptosis by Altering the Balance of Proapoptotic and Antiapoptotic Genes in Breast Cancer Cells and Inhibits Tumor Sphere Formation

“…They contain diverse biomolecules that are used for the treatment of various diseases including cancer [5]. Although a number of studies have already been conducted to explore the antibacterial and antifungal [6], anti-HIV [7], antitumoral [8], cytotoxic activity [9], antilipidemic [10], hyperglycemic and hypotensive [11], antiinflammatory [12], and immunomodulatory [13] properties of some species of oyster mushrooms, no reports have been published indicating the anticancer properties of the P. highking mushroom.…”

Mycosynthesis and characterization of silver nanoparticles from Pleurotus djamor var. roseus and their in vitro cytotoxicity effect on PC3 cells

“…They belong to the oyster mushroom family (Pleurotaceae) and are known for being edible and nutritious mushrooms [13]. Fruiting bodies as well as active mycelia of Pleurotus species also possess a number of therapeutic properties such as antiinflammatory, immunostimulatory and immunomodulatory [14], anticancer [15], ribonuclease [16], antiviral [17], anti HIV [18], antitumor [19] and antibacterial activities [20]. The photo-irradiated extracellular synthesis of AgNPs using an aqueous extract of the edible oyster mushroom Pleurotus florida as a reducing agent has been reported earlier [10].…”

THE NUTRITIONAL AND HEALTH PROPERTIES OF AN OYSTER MUSHROOM (Pleurotus ostreatus (Jacq. Fr) P. Kumm.)

“…A direct induction of cancer cells apoptosis, as well as activation of immunological response of an organism were observed [Sarangi et al 2006]. A strong cytostatic effect of water and alcohol extracts towards human lung cancer cells (A549), cervical cancer (SiHa and HeLa), colorectal cancer (HT-29) [Lavi et al 2006], leukemia (HL-60) [Venkatakrishnana et al 2010], as well as a mouse sarcoma (S-180) was proven [Li et al 2008, Maiti et al 2011. Fig.…”

“…Among the phenolic acids identified in oyster mushroom sporocarps, protocatechuic acid shows a strong anticoagulant activity [Alam et al 2011]. Santoyo et al [2012] polysaccharide extract Wolf et al [2008] pulverized mushrooms Arora and Tandon [2015] polysaccharide fraction, glucan Silva et al [2012], Cao et al [2015] proteoglycans Sarangi et al [2006] protein Maiti et al [2011], Wu et al [2011] Anti-neoplastic lectin Lavi et al [2006] water-soluble polysaccharide (POPS-1) Choi et al [2004] ethanol extract Gu and Sivam [2006], Sarangi et al [2006], Venkatakrishnana et al [2010] Anti-tumor immunomodulatory Cibacron Blue F3GA, protein fraction Maiti et al [2011] pleuran (ß-1, 3-glucan with galactose and mannose) water and ethanolic extract (30%)…”