Antitumor and Immunostimulatory Effects of Residual Powder from Barley-Shochu Distillation Remnants

Abstract

The development of effective utilization methods of Shochu distillation remnants is desirable from the viewpoint of environmental protection and biomass. In this study, we examined inhibitory effects of the powder (barleypowder) obtained from the barley-Shochu distillation remnants on the various tumor cells in vitro and immunostimulation effect in vivo. Remarkable inhibitory effects on the growth of human lung tumor cells along with the induction of apoptosis were obtained for barley-powder in vitro. On the other hand, the barley-powder induced the production of serum Interferon (IFN)-γ for normal rats in vivo, and increased natural killer (NK) activity. Furthermore, the barley-powder showed no acute toxicity in the tail vein administration to normal rats. It was found that the barley-powder had not only an antitumor effect but also an immunostimulation effect for the first time.

Residual powders from Shochu distillation remnants induce apoptosis in human hepatoma cells via the caspase-independent pathway

“…We have previously reported that PSDR showed anticancer effects against various cancer cells in vitro (15,16). However, a comparative study on the anticancer effects of different PSDR has not been performed.…”

“…The result is shown in Fig. 2 (15,16). So, we investigated the induction of apoptosis in HepG2 and HuH-7 cells by barley-, rice-, and sweet potato-PSDR on the basis of fluorescence microscopic and flow cytometric analyses.…”

“…In addition, rice-PSDR showed an effective growth inhibition of cancer cells along with the induction of apoptosis in vitro (15). Recently, we found that immunostimulatory effects of barley-PSDR on the serum interferon (IFN)-g production and natural killer (NK) activity without toxicity to normal rats in vivo (16). However, a comparative study on the anticancer effects of different PSDR such as barley-, rice-, and sweet potato-PSDR has not been performed.…”

Dietary supplementation with Lactobacillus plantarum dy‐1 fermented barley suppresses body weight gain in high‐fat diet‐induced obese rats

“…Powder obtained from the remnants of barley‐shochu distillation showed no acute toxicity in tail vein administration to normal rats. Furthermore, the barley powder was shown to have not only an antitumor effect but also an immunostimulatory effect . Giriwono et al demonstrated a novel approach for the use of fermented barley extracts (FBE) with Koji ( Aspergillus kawauchi ) as a supplement to attenuate chronic alcohol consumption.…”

Anticancer Effects of Residual Powder from Barley-<i>Shochu</i> Distillation Remnants against the Orthotopic Xenograft Mouse Models of Hepatocellular Carcinoma <i>in Vivo</i>

“…We have already reported on inhibitory effects of freeze-dried supernatants of barley-, rice-, and sweet potato-SDR on the growth of human tumor cells in vitro. 10) Furthermore, we have obtained valuable powder from Shochu distillation remnants (PSDR) by treatment with ethanol. In addition, we found immunostimulatory effects of barley-PSDR on serum interferon (IFN)-γ production and natural killer (NK) activity without toxicity to normal rats in vivo.…”