Antitumor and immunomodulatory effects of Justicia spicigera Schltdl (Acanthaceae)

Alonso-Castro, Ángel Josabad; Ortíz‐Sánchez, Elizabeth; Domínguez, Fabiola; Arana-Argáez, Víctor; Juárez-Vázquez, María del Carmen; Chávez, Marı́a Isabel; Carranza‐Álvarez, Candy; Gaspar-Ramírez, Octavio; Espinosa-Reyes, Guillermo; López-Toledo, Gabriela; Ortíz-Andrade, Rolffy; García‐Carrancá, Alejandro

doi:10.1016/j.jep.2012.03.036

Cited by 47 publications

(30 citation statements)

References 21 publications

(15 reference statements)

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“…The immune system is the human's ultimate defence against infectious diseases, tumour and cancer growth . Cancer remains a formidable problem for people since the majority of chemical compounds that have been identified as cytotoxic to cancer cells are also toxic to normal cells . In a cancer regimen, immunosuppression remains the main challenge hence the need for immunostimulating anti‐tumour therapies.…”

Section: Discussionmentioning

confidence: 99%

“…Release of NO by macrophages was measured by the amounts of accumulated nitrite (NO 2 − ) in cell‐free supernatants via the Griess reaction . Cells were suspended in DMEM, plated in 96‐well culture plates (2 × 10 5 cells mL −1 ) and incubated for 48 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Hydrogen peroxide release by macrophages was determined according to Alonso‐Castro et al Following treatment with PEQP fractions and control, macrophage cell culture supernatants (100 µL) were mixed with equal volumes of fresh phenol red solution per well in a 96‐well plate and incubated for 3 h. The reaction was stopped by adding 10 µL of 1 mol L −1 NaOH solution into each well and the absorbance was spectrophotometrically read at 620 nm. H 2 O 2 concentration was determined by a H 2 O 2 (0–40 µmol L −1 ) standard curve and was expressed as µmol L −1 .…”

Section: Methodsmentioning

confidence: 99%

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Antiproliferative and immunostimulatory activity of a protein from Pleurotus eryngii

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Antiproliferative and immunostimulatory activity of a protein from Pleurotus eryngii

et al. 2014

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“…Thus, the therapeutic benefits of J. spicigera may also be partly due to its ability to decrease the generation of reactive oxygen species, a process that results in oxidative stress and aggravates the diabetic state. The JSE doses used in this study were selected based on their lack of toxic effects, as evaluated in acute toxicity test and on preliminary studies carried out in our laboratory (Alonso-Castro et al, 2012). It is important to point out that in all in vivo experiments the hypoglycemic activities of JSE were similar to those produced by the positive control glibenclamide, which causes hypoglycemia by enhancing insulin secretion in pancreatic b-cells.…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic effects of Justicia spicigera Schltdl (Acanthaceae)

Ortíz-Andrade¹,

Cabañas-Wuan²,

Arana-Argáez³

et al. 2012

Journal of Ethnopharmacology

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“…The erythrocytes were lysed with red blood cell lysis buffer, while the remaining cells were suspended in RPMI-1640 medium with 10% fetal calf serum. Cells were counted with a hemocytometer using the trypan blue exclusion technique (14). Cell viability exceeded 95% and cells were resuspended with complete medium at a density of 1x10 10 cells/l.…”

Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

Antitumor activity of yulangsan polysacchrides in mice bearing S180 sarcoma tumors

Cai

Chen

Pan

et al. 2017

Molecular and Clinical Oncology

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Abstract. Sarcoma is one of the most prevalent pediatric tumors and the therapeutic role of chemotherapy has yet to be elucidated. It has been reported that extracts of Longyanshen (Yulangsan) may enhance the sensitivity of drug-resistant cancer cell lines, and improve the immune dysfunction induced by cyclophosphamide (CTX) in mice. The present in vivo study investigated the antitumor effects of Yulangsan polysaccharides (YLSPS) and their interaction with CTX in murine sarcoma 180 (S180)-bearing mice. Immunohistochemistry was used to detect the expression of apoptosis-related proteins. The ultrastructure of sarcoma cells was examined by transmission electron microscopy and the tumor growth rate was determined by measuring the tumor weight. A dose-dependent inhibition of sarcoma growth was observed in S180-bearing mice following administration of YLSPS. In combination with CTX, an additive antitumor effect was obtained, which was accompanied by amelioration of immune function. YLSPS also potentiated the tumor suppression effect of CTX while avoiding cytotoxicity against immune cells. YLSPS inhibited sarcoma growth in S180-bearing mice through the induction of apoptosis in S180 sarcoma cells. YLSPS also attenuated CTX-induced cytotoxicity to the immune system while potentiating the tumor suppression effect. These results provide additional information regarding combination therapy with YLSPS and chemotherapy for the treatment of sarcoma. IntroductionSarcomas are a heterogeneous group of tumors that are mesenchymal in origin and account for 1% of adult tumors and 15% of pediatric tumors (1,2). Approximately 80% of sarcomas originate from soft tissue and 20% from bone. Similar to other types of solid tumors, conventional therapy includes surgery, radiotherapy and chemotherapy. The role of adjuvant or neoadjuvant chemotherapy for sarcoma has not yet been fully elucidated, as large prospective analyses have not demonstrated their unequivocal benefit (3,4); however, a previous meta-analysis suggested that adjuvant chemotherapy is associated with a moderate overall survival advantage (5). A number of agents are currently used for sarcoma therapy, including doxorubicin, ifosfamide, dacarbazine, cisplatin, vincristine, cyclophosphamide (CTX) and etoposide. Under most circumstances, the combination of ≥2 agents is commonly used in patients with sarcoma, as combination chemotherapy appears to increase response rates; however, it is associated with greater toxicity, with immunosuppression being one of the most serious side effects, with no overall survival advantage (6).Yulangsan, also referred to as Longyanshen, is a commonly used herb in the indigenous Zhuang community in Guangxi, China, and is derived from the dried roots of Millettia pulchra Kurz var-laxior (Dunn) Z. We. Locally, this herb is used for patients recuperating after illness and those concerned about their general health. In the published literature, it has been reported that Yulangsan may enhance the function of the immune system (7). Kong et al found that ...

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Antitumor and immunomodulatory effects of Justicia spicigera Schltdl (Acanthaceae)

Cited by 47 publications

References 21 publications

Antiproliferative and immunostimulatory activity of a protein from Pleurotus eryngii

Antiproliferative and immunostimulatory activity of a protein from Pleurotus eryngii

Antidiabetic effects of Justicia spicigera Schltdl (Acanthaceae)

Antitumor activity of yulangsan polysacchrides in mice bearing S180 sarcoma tumors

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