Antitumor and Apoptosis Induction Effects of Paeonol on Mice Bearing EMT6 Breast Carcinoma

Ou, Yetao; Li, Qingwang; Wang, Jianjie; Li, Kun; Zhou, Shaobo

doi:10.4062/biomolther.2013.106

Cited by 34 publications

(26 citation statements)

References 13 publications

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“…Previous studies have demonstrated that the mechanism of Pae's antitumor effect is via induction of apoptosis, stimulation of IL-2, and TNF-α production. 5,12,35 In the current study, we showed that Pae-induced apoptosis is closely related to the induction of ROS and the suppression of Akt activation in human lung cancer cells. Furthermore, drug-loaded nanoparticles exhibit clear A similar study is ongoing in the authors' laboratory, focusing on the synergistic effect and the underlying mechanisms of Pae and paclitaxel in several cancer cell lines.…”

Section: Modulation Of Akt/apoptotic Pathways By Pae-npsmentioning

confidence: 50%

Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

Chen¹,

Feng²,

Hou³

et al. 2017

IJN

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Paeonol (Pae; 2′-hydroxy-4′-methoxyacetophenone) has attracted intense attention as a potential therapeutic agent against various cancers. However, the use of Pae is limited owing to its hydrophobicity. Recently, biodegradable polymeric nanoparticles with amphiphilic copolymers have been used as drug carriers; these have better bioavailability and are promising tumor-targeted drug delivery systems. In the current study, we prepared Pae-loaded nanoparticles (Pae-NPs) with amphiphilic block copolymers using nanoprecipitation. The physiochemical characteristics and antitumor effects of nanoparticles were evaluated in different cancer cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed substantial inhibition of cell growth by Pae-NPs. Moreover, lower doses of Pae-NPs inhibited cell growth more efficiently than the equivalent doses of free Pae. Inhibition was characterized by significant elevation of intracellular reactive oxygen species and subsequent inhibition of Akt and regulation of apoptotic proteins, which could be partly reversed by pretreatment with the antioxidant N-acetylcysteine. In vivo results also demonstrated that Pae-NPs could exert much stronger antitumor effects than free Pae. Therefore, Pae-NPs represent a promising delivery system to overcome the low solubility of Pae and enable its use in treating cancer.

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Section: Modulation Of Akt/apoptotic Pathways By Pae-npsmentioning

confidence: 50%

Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

Chen¹,

Feng²,

Hou³

et al. 2017

IJN

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“…[9][10][11] Previous studies suggested that Paeonol possesses anti-proliferation, apoptosis induction, and cell cycle arresting effects in various types of cancers, such as breast, colorectal, lung, ovarian, gastric cancer, etc. 9,[12][13][14][15][16][17][18][19][20][21] More recent studies reported that Paeonol enhanced paclitaxel-induced apoptosis in breast cancer cells, 20,22 reversed Doxorubicin resistance in liver cancer, 23 and enhanced the radiosensitivity of lung cancer cells. 24 Studies also reported the anti-metastatic activities of Paeonol in human fibrosarcoma, chondrosarcoma and gastric cancer.…”

Section: Introductionmentioning

confidence: 99%

<p>Paeonol Inhibits Pancreatic Cancer Cell Migration and Invasion Through the Inhibition of TGF-β1/Smad Signaling and Epithelial-Mesenchymal-Transition</p>

Cheng

Chen

Tang

et al. 2020

CMAR

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These authors contributed equally to this workPurpose: Paeonol, a natural product derived from the root of Cynanchum paniculatum (Bunge) K. Schum and the root of Paeonia suffruticosa Andr. (Ranunculaceae) has attracted extensive attention for its anti-cancer proliferation effect in recent years. The present study examined the role of paeonol in suppressing migration and invasion in pancreatic cancer cells by inhibiting TGF-β1/Smad signaling. Methods: Cell viability was evaluated by MTT and colonial formation assay. Migration and invasion capabilities were examined by cell scratch-wound healing assay and the Boyden chamber invasion assay. Western Blot and qRT-PCR were used to measure the protein and RNA levels of vimentin, E-cadherin, N-cadherin, and TGF-β1/Smad signaling. Results: At non-cytotoxic dose, 100 μΜ and 150 μΜ of paeonol showed significant antimigration and anti-invasion effects on Panc-1 and Capan-1 cells (p<0.01). Paeonol inhibited epithelial-mesenchymal-transition by upregulating E-cadherin, and down regulating N-cadherin and vimentin expressions. Paeonol inhibited TGF-β1/Smad signaling pathway by downregulating TGF-β1, p-Smad2/Smad2 and p-Smad3/Smad3 expressions. Further, TGF-β1 attenuated the anti-migration and anti-invasion capacities of paeonol in Panc-1 and Capan-1 cells. Conclusion: These findings revealed that paeonol could suppress proliferation and inhibit migration and invasion in Panc-1 and Capan-1 cells by inhibiting the TGF-β1/Smad pathway and might be a promising novel anti-pancreatic cancer drug.

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“…Unlike cytotoxic drugs, paeonol has shown cardioprotective, 16 hepatic-protective, 17−19 renal protective 20 and neuroprotective effects. 21 Paeonol has been shown to exhibit antitumor activities on gastric, 22,23 prostate, 24 breast, 25 hepar, 26 ovarian 27 and esophageal cancer cells. 28 Our previous study also revealed that paeonol exhibited the inhibiting effects on colorectal cancer lines (HCT116, SW620 and LoVo), especially in the LoVo cells.…”

Section: Introductionmentioning

confidence: 99%

Paeonol suppresses invasion, migration, and epithelial-to-mesenchymal transition in colorectal cancer cells through inhibition of COX-2 and PGE2

Sheng

2020

Preprint

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Purpose: To observe the effects of paeonol on the invasion and migration of LoVo colorectal cancer cells, and investigate its possible mechanisms. Materials and Methods: Cell transwell assay and wound-healing assay were applied, and the results suggested that paeonol could significantly inhibit the invasion and migration abilities of LoVo cells, which was associated with a reduction in COX-2 expression and PGE2 synthesis. Treatment with the selective COX-2 inhibitor, celecoxib, or transient transfection of colorectal cancer cells with COX-2 siRNA, also inhibited cell invasion and migration. Results: The invasion and migration capacity were evaluated in LoVo cells by transwell assay and wound-healing in vitro. Compared with the control group, the invasion cells through Matrigel and the wound-healing rate were significantly decreased after treated with paeonol for 24 h. Paeonol treatment downregulated the expression of MMP-9 and downregulated the COX-2 expression and PGE2 synthesis in LoVo cells. Paeonol could up-regulate the expression of epithelial marker E-cadherin while down-regulate the expressions of mesenchymal markers, Fibronectin and Vimentin. Paeonol inhibited PI3K-Akt and MAPK-ERK pathways in LoVo cells. Celecoxib treatment significantly decreased the cells penetrating the matrigel in a dose-dependent manner. siRNA knockdown of COX-2 leaded to inhibition of cell invasion in LoVo cells. Knockdown of COX-2 increased the expression of E-cadherin, whereas the expressions of Fibronectin and Vimentin were downregulated. Conclusion: Paeonol may inhibit PI3K-Akt and MAPK-ERK pathways through suppressing of the COX-2 expression and PGE2 synthesis, thus inhibiting the cell invasion, migration, and EMT in LoVo cells.

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Antitumor and Apoptosis Induction Effects of Paeonol on Mice Bearing EMT6 Breast Carcinoma

Cited by 34 publications

References 13 publications

Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

<p>Paeonol Inhibits Pancreatic Cancer Cell Migration and Invasion Through the Inhibition of TGF-β1/Smad Signaling and Epithelial-Mesenchymal-Transition</p>

Paeonol suppresses invasion, migration, and epithelial-to-mesenchymal transition in colorectal cancer cells through inhibition of COX-2 and PGE2

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