Antitumor agents. 33. Isolation and structural elucidation of bruceoside -A and -B, novel antileukemic quassinoid glycosides, and brucein -D and -E from Brucea javanica

Lee, Kuo Hsiung̀; Imakura, Yasuhiro; Sumida, Yoshio; Wu, Rong Yang; Hall, Iris H.; Huang, Huan Chang

doi:10.1021/jo01327a031

Cited by 90 publications

(32 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Beberapa penelitian sebelumnya telah menunjukkan bahwa senyawa quassinoid dari tanaman ini mempunyai aktivitas antitumor. [3][4][5] Senyawa golongan quassinoid itu dapat menginduksi apoptosis sehingga terjadi degradasi DNA menjadi rantai oligonukleosom. 6 Penelitian kami sebelumnya membuktikan bahwa senyawa brusein-A yang diisolasi dari buah makasar menunjukkan aktivitas antikanker secara in vitro terhadap kanker payudara dengan nilai IC50 0,54 mg/L tidak berbeda nyata dengan standar obat cisplatin yang mempunyai nilai IC50 0,43 mg/L.…”

Section: Pendahuluanunclassified

Ekspresi <i>Caspase-3</i> pada Kanker Payudara Tikus Setelah Pemberian Antikanker Brusein-A

Muhartono

subeki

2017

GMHC

View full text Add to dashboard Cite

AbstrakBrusein-A diduga menyebabkan apoptosis. Salah satu protein yang berperan dalam proses apoptosis adalah caspase-3. Penelitian ini bertujuan mengetahui aktivitas antikanker brusein-A terhadap ekspresi caspase-3 pada kanker payudara. Penelitian menggunakan rancang acak lengkap. Sebanyak 27 ekor tikus betina berumur 12 minggu diberi dimethylbenzanthracene (DMBA) 20 mg/kgBB per oral selama 3 minggu sampai terbentuk kanker payudara. Selanjutnya, dibagi dalam 9 kelompok perlakuan brusein-A, yaitu 0; 2, 5; 5; 7,5; 10; 12,5; 15; 17,5; dan 20 mg/L selama 28 hari. Parameter yang diukur adalah ekspresi caspase-3 yang dinilai berdasar atas persentase sitoplasma yang berwarna coklat. Penelitian ini dilakukan di Laboratorium Patologi-Anatomi dan Laboratorium Biokimia, Pusat Penelitian Ilmu Pengetahuan dan Teknologi (Puspiptek) Serpong tahun 2015-2016. Hasil penelitian menunjukkan ekspresi caspase-3 rata-rata pada dosis 0 mg/L sebesar 4%, 2,5 mg/L sebesar 15,3%, 5 mg/L sebesar 21%, 7,5 mg/L sebesar 25%, 10 mg/L sebesar 41%, 12,5 mg/L sebesar 65%, 15 mg/L sebesar 75,3%, 17,5 mg/L sebesar 84%, dan 20 mg/L sebesar 94,7%. Hasil uji one way ANOVA menunjukkan perbedaan ekspresi caspase-3 rata-rata yang signifikan antarkelompok perlakuan (p=0,0001). Uji korelasi Spearman menunjukkan hubungan yang sangat erat dan positif antara dosis brusein-A dan ekspresi caspase-3 (r=0,994). Simpulan, brusein-A meningkatkan ekpresi caspase-3 pada kanker payudara tikus yang diinduksi DMBA. Kata kunci: Brusein-A, caspase-3, kanker payudara Caspase-3 Expression on Breast Cancer Rats After Brusein-A Administration AbstractBrusein-A is thought to cause apoptosis. Caspase-3 is a protein that plays a role in the process of apoptosis. This study aims to determine anticancer activity of brusein-A on the expression of caspase-3 in breast cancer. This study uses a completely randomized control design. A total of 27 female rats, 12 week aged, were given 20 mg dimethylbenzanthracene (DMBA)/kgBW peroral for 3 weeks until they had breast cancer. They divided into 9 treatment group of brusein A, that were 0, 2.5, 5, 7.5, 10, 12.5, 15, 17.5, and 20 mg/L for 28 days. Parameter measured were caspase-3 expression, assessed on the percentage of brown cytoplasm. This research was conducted in Pathology-Anatomy Laboratory and Biochemistry Laboratory, Research Center for Science and Technology (Puspiptek) Serpong in 2015-2016. The results showed caspase expression rate of 4%, 15.3%, 21%, 25%, 41%, 65%, 75.3%, 84%, and 94.7% on the dosage of , 2.5, 5, 7.5, 10, 12.5, 15, 17.5, and 20 mg/L respectively. The one way ANOVA test results showed significant difference of caspase-3 expression between treatment group (p=0.0001). Spearman's rank correlation test showed that a very close and positive relationship between brusein-A dose and caspase-3 expression (r=0.994). In conclusion, brusein-A increased caspase-3 expression in DMBA induced breast cancer rats.

show abstract

Section: Pendahuluanunclassified

Ekspresi <i>Caspase-3</i> pada Kanker Payudara Tikus Setelah Pemberian Antikanker Brusein-A

Muhartono

subeki

2017

GMHC

View full text Add to dashboard Cite

show abstract

“…The structures of other sixteen known compounds were identified by comparing their spectroscopic data (UV, MS, 1 H and 13 C NMR) with those of reported values and found to be bruceine D (3) [19], yadanzioside E (4) [20], brusatol (5) [21], bruceoside B (6) [22], bruceantinol (7) [23], yadanzioside K (8) [23], P (9) [24], bruceine A (10) [20], yadanzioside B (11) [20], bruceantarin (12) [25], bruceoside A (13) [26], yadanzioside C (14) [20], G (15) [20], F (16) [27], A (17) [20], and M (18) [23].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Isolation, chemotaxonomic significance and cytotoxic effects of quassinoids from Brucea javanica

Bai

et al. 2015

Fitoterapia

View full text Add to dashboard Cite

“…Other quassinoids [e.g., brusatol (H2)] and quassinoid glycosides [e.g., bruceoside B (H3)] from B. javanica have been studied extensively for antitumor [89][90][91][92] and cancer chemopreventive [93] effects. A recent review of biologically active quassinoids discussed their potential for drug design [94].…”

Section: Anticancer and Antitumor Compoundsmentioning

confidence: 99%

Plant-derived natural product research aimed at new drug discovery

et al. 2008

Self Cite

View full text Add to dashboard Cite

Many important bioactive compounds have been discovered from natural sources using bioactivity-directed fractionation and isolation (BDFl) [Balunas MJ, Kinghorn AD (2005) Drug discovery from medicinal plants. Life Sci 78:431-441]. Continuing discovery has also been facilitated by the recent development of new bioassay methods. These bioactive compounds are mostly plant secondary metabolites, and many naturally occurring pure compounds have become medicines, dietary supplements, and other useful commercial products. Active lead compounds can also be further modified to enhance the biological profiles and developed as clinical trial candidates. In this review, the authors will summarize research on many different useful compounds isolated or developed from plants with emphasis placed on those recently discovered by the authors' laboratories as antitumor and anti-HIV clinical trial candidates.

show abstract

Antitumor agents. 33. Isolation and structural elucidation of bruceoside -A and -B, novel antileukemic quassinoid glycosides, and brucein -D and -E from Brucea javanica

Cited by 90 publications

References 6 publications

Ekspresi <i>Caspase-3</i> pada Kanker Payudara Tikus Setelah Pemberian Antikanker Brusein-A

Ekspresi <i>Caspase-3</i> pada Kanker Payudara Tikus Setelah Pemberian Antikanker Brusein-A

Isolation, chemotaxonomic significance and cytotoxic effects of quassinoids from Brucea javanica

Plant-derived natural product research aimed at new drug discovery

Contact Info

Product

Resources

About