Antitumor Activity of Some Polysaccharides Isolated from an Edible Mushroom,<i>Ningyotake</i>, the Fruiting Body and the Cultured Mycelium of<i>Polyporous confluens</i>

Mizuno, Takashi; Ando, Motoharu; Sugie, Reiko; Ichikawa, Hitoshi; Shimura, Keishiro; Sumiya, Takahiro; Matsuura, Akira

doi:10.1271/bbb.56.34

Cited by 43 publications

(19 citation statements)

References 4 publications

(5 reference statements)

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“…For example, sclerotia extracts of I. obliquus are known to inhibit the growth and protein synthesis of tumor cells. Additionally, Mizuno (1992Mizuno ( , 1999 reported that polysaccharides from fungal sclerotia, which are known to include hetero-polysaccharide and homoglycan, showed strong antitumor effects, while polysaccharides from cultured mycelia did not. However, Kim et al (2005Kim et al ( , 2006 reported that the polysaccharides extracted from cultivated mycelia (termed endo-polysaccharides) also have anticancer activity.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, Kim et al (2006) reported that watersoluble polysaccharides from the sclerotia of I. obliquus were hetero-polysaccharides of xylogalactoglucan composed primarily of glucose, galactose and xylose, with low contents of arabinose and fucose. The presence of protein has been reported to be important for biological activities (Mizuno et al, 1992), and is especially important for the biological activities of hetero-polysaccharides. However, Kim et al (2005Kim et al ( , 2006 demonstrated that proteins combined with endo-polysaccharides were unlikely to affect the biological activity.…”

Section: Discussionmentioning

confidence: 99%

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Antitumor activity of water extract of a mushroom, Inonotus obliquus, against HT‐29 human colon cancer cells

Lee

Hwang

Yun

2009

Phytotherapy Research

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In the current study, it was demonstrated that the hot water extract of I. obliquus (IOWE) exerts inhibitory activity against the proliferation of human colon cancer cells (HT-29). The inhibitory effect of IOWE on the growth of HT-29 cancer cells was evaluated by treating cells with IOWE at concentrations of 0.25, 0.5 and 1.0 mg/mL for 24 or 48 h. The IOWE inhibited cell growth in a dose-dependent manner, and this inhibition was accompanied by apoptotic cell death. The maximum inhibitory effect (56%) was observed when IOWE was treated at a concentration of 1.0 mg/mL for 48 h. The apoptotic effect of IOWE on HT-29 cells was also confirmed by flow cytometric analysis. In addition, the apoptotic cell percentage was closely associated with down-regulation of Bcl-2 and up-regulation of Bax and caspase-3. The results suggest that IOWE would be useful as an antitumor agent via the induction of apoptosis and inhibition of the growth of cancer cells through up-regulation of the expression of proapoptotic proteins and down-regulation of antiapoptotic proteins.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antitumor activity of water extract of a mushroom, Inonotus obliquus, against HT‐29 human colon cancer cells

Lee

Hwang

Yun

2009

Phytotherapy Research

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“…The biomass (100 g) produced was extracted to obtain the endopolysaccharides using modified Mizuno method [8], involving hot water extraction for at least 2 h, filtration, concentration process and centrifugation. The supernatant was added to absolute ethanol (ratio 1:1) and kept overnight before lyophilization to get the polysaccharides.…”

Section: Hot Water Extraction To Produce Endopolysaccharidesmentioning

confidence: 99%

Production of Endopolysaccharides from Malaysia’s Local Mushrooms in Air-Lift Bioreactor

Mohamad¹,

Awang²,

Ibrahim³

et al. 2015

ABB

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Four local mushroom species, viz. Auricularis polytricha, Lentinus edodes, Agrocybe sp and Pleurotus flabellatus were grown under submerged culture and screened for endopolysaccharides. The fermentation was done in 250 ml working volume Erlenmeyer flask and the fermentation curves for all species were established. Pleurotus flabellatus has the highest rate of biomass production at the rate of 0.180 g/L/day, at 10 days hence chosen for further investigation. Two additional media, viz. Mushroom Complete Media (MCM) and Yeast Malt (YM) were selected to be compared with potato extract(PE) media used initially. MCM media produced the highest biomass productivity at the rate of 0.311 g/L/day. Pleurotus flabellatus biomass was extracted using modified Mizuno method and the endopolysaccharide obtained was tested for β-glucan. The yield of β-glucan was 7.70 ± 1.11 g/100g. The polysaccharides were purified using column chromatography to yield four fractions. The fourth fraction F4, gave the highest molecular weight at 3.058 × 10 6 Dalton (11.8%) and 1.282 × 10 4 Dalton (88.2%). The mushroom, P. flabbelatus was cultured using air-lift bioreactor, and the highest productivity was obtained at air-flowrate 2 L/min, yielding 2.25 g/L/day. The yield of biomass against substrate used (glucose consumption) Y b/s was 0.78 g/g.

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“…A variety of compounds with important pharmacological properties have been isolated from mushrooms [3][4][5]. Among them, the study of mushroom lectins with immunomodulatory, antiproliferative, antitumour/cytotoxic and hypotensive activities has attracted growing interest [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Crystallization and Preliminary Crystallographic Studies of an Antitumour Lectin from the Edible Mushroom Agrocybe aegerita

Yang¹,

Liang²,

Xiang³

et al. 2005

PPL

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An antitumour lectin named AAL has been purified from the fruiting body of edible mushroom Agrocybe aegerita. In addition to having a distinct bioactivity, AAL shows strong inhibition effects on human and mouse tumour cells. It has been shown that AAL exerts its antitumour effects via apoptosis-induction. AAL and AAL-lactose complex have been crystallized and their diffraction data were collected with resolution of 2.6 Å and 3.0 Å, respectively. Both crystals belong to space group P 6122 with unit cell parameters a = 123.98 Å, b = 123.98 Å, c = 56.86 Å, α= β=90˚, γ = 120˚ and a = 123.69 Å, b = 123.69 Å, c = 56.64 Å, α= β=90˚, γ = 120˚, respectively.

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Antitumor Activity of Some Polysaccharides Isolated from an Edible Mushroom,Ningyotake, the Fruiting Body and the Cultured Mycelium ofPolyporous confluens

Cited by 43 publications

References 4 publications

Antitumor activity of water extract of a mushroom, Inonotus obliquus, against HT‐29 human colon cancer cells

Antitumor activity of water extract of a mushroom, Inonotus obliquus, against HT‐29 human colon cancer cells

Production of Endopolysaccharides from Malaysia’s Local Mushrooms in Air-Lift Bioreactor

Crystallization and Preliminary Crystallographic Studies of an Antitumour Lectin from the Edible Mushroom Agrocybe aegerita

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