Antitumor activity of silver nanoparticles in Dalton&amp;rsquo;s lymphoma ascites tumor model

Sriram, Muthu Irulappan; Kanth, Selvaraj Barath Mani; Kalishwaralal, Kalimuthu; Gurunathan, Sangiliyandi

doi:10.2147/ijn.s11727

Cited by 217 publications

(71 citation statements)

References 35 publications

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“…Color formation rapidly occurred in this mixture, possibly because of the reaction between Ag ions and NAR [12,28]. The synthesis of AgNPs was confirmed using UV–visible spectroscopy (UV–vis).…”

Section: Resultsmentioning

confidence: 99%

“…Gurunathan et al demonstrated the anti-angiogenic properties of AgNPs in vascular endothelial cells, which showed equivalent potency to a natural anti-angiogenic molecule, known as pigment epithelium-derived factor, by inhibiting the phosphorylation of protein kinase B/Akt [11]. SriRam et al demonstrated the potential cytotoxicity against Dalton’s lymphoma ascites (DLA) and DLA-induced tumors in mice, with significantly increased survival times in the tumor mouse model compared to the untreated group [12]. Low concentrations of poly ( N -vinyl-2-pyrrolidone)-coated AgNPs inhibited the viability of acute myeloid leukemia cells and the viability of K562 cells in a dose-dependent manner after entering the cells via endocytic pathway endosomes [13].…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic Potential and Molecular Pathway Analysis of Silver Nanoparticles in Human Colon Cancer Cells HCT116

Gurunathan

Qasim

Park

et al. 2018

IJMS

137

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Silver nanoparticles (AgNPs) have gained attention for use in cancer therapy. In this study, AgNPs were biosynthesized using naringenin. We investigated the anti-colon cancer activities of biogenic AgNPs through transcriptome analysis using RNA sequencing, and the mechanisms of AgNPs in regulating colon cancer cell growth. The synthesized AgNPs were characterized using UV–visible spectroscopy (UV–vis), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and transmission electron microscopy (TEM). The AgNPs were spherical with sizes of 2–10 nm. Cytotoxicity assays indicated that the AgNPs in HCT116 colorectal cancer cells were very effective at low concentrations. The viability and proliferation of colon cancer cells treated with 5 µg/mL biogenic AgNPs were reduced by 50%. Increased lactate dehydrogenase leakage (LDH), reactive oxygen species (ROS) generation, malondialdehyde (MDA), and decreased dead-cell protease activity and ATP generation were observed. This impaired mitochondrial function and DNA damage led to cell death. The AgNPs upregulated and downregulated the most highly ranked biological processes of oxidation–reduction and cell-cycle regulation, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that AgNPs upregulated GADD45G in the p53 pathway. Thus, the AgNP tumor suppressive effects were mediated by cell apoptosis following DNA damage, as well as by mitochondrial dysfunction and cell-cycle arrest following aberrant regulation of p53 effector proteins. It is of interest to mention that, to the best of our knowledge, this study is the first report demonstrating cellular responses and molecular pathways analysis of AgNPs in HCT116 colorectal cancer cells.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxic Potential and Molecular Pathway Analysis of Silver Nanoparticles in Human Colon Cancer Cells HCT116

Gurunathan

Qasim

Park

et al. 2018

IJMS

137

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“…The IC 50 value of biosynthesized AgNPs against HeLa cells at 200 μ g/mL concentrations (Figure 6(a)). Previously, synthesized AgNPs inducing cytotoxicity were discussed by Sriram et al [42] and Safaepour et al [43]. …”

Section: Resultsmentioning

confidence: 99%

Biosynthesis, Antimicrobial and Cytotoxic Effect of Silver Nanoparticles Using a NovelNocardiopsissp. MBRC-1

Manivasagan

Venkatesan

Kalimuthu

et al. 2013

BioMed Research International

191

121

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The biosynthesis of nanoparticles has been proposed as a cost effective environmental friendly alternative to chemical and physical methods. Microbial synthesis of nanoparticles is under exploration due to wide biomedical applications, research interest in nanotechnology and microbial biotechnology. In the present study, an ecofriendly process for the synthesis of nanoparticles using a novel Nocardiopsis sp. MBRC-1 has been attempted. We used culture supernatant of Nocardiopsis sp. MBRC-1 for the simple and cost effective green synthesis of silver nanoparticles. The reduction of silver ions occurred when silver nitrate solution was treated with the Nocardiopsis sp. MBRC-1 culture supernatant at room temperature. The nanoparticles were characterized by UV-visible, TEM, FE-SEM, EDX, FTIR, and XRD spectroscopy. The nanoparticles exhibited an absorption peak around 420 nm, a characteristic surface plasmon resonance band of silver nanoparticles. They were spherical in shape with an average particle size of 45 ± 0.15 nm. The EDX analysis showed the presence of elemental silver signal in the synthesized nanoparticles. The FTIR analysis revealed that the protein component in the form of enzyme nitrate reductase produced by the isolate in the culture supernatant may be responsible for reduction and as capping agents. The XRD spectrum showed the characteristic Bragg peaks of 1 2 3, 2 0 4, 0 4 3, 1 4 4, and 3 1 1 facets of the face centered cubic silver nanoparticles and confirms that these nanoparticles are crystalline in nature. The prepared silver nanoparticles exhibited strong antimicrobial activity against bacteria and fungi. Cytotoxicity of biosynthesized AgNPs against in vitro human cervical cancer cell line (HeLa) showed a dose-response activity. IC50 value was found to be 200 μg/mL of AgNPs against HeLa cancer cells. Further studies are needed to elucidate the toxicity and the mechanism involved with antimicrobial and anticancer activity of the synthesized AgNPs as nanomedicine.

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“…[33][34][35][36][37][38] Ag NPs are also potent sensitizers for cancer chemotherapy 39 and radiotherapy, 40,41 and show significant antitumor activity in several animal models. [42][43][44] However, whether Ag NPs can induce autophagy and how this might affect the anticancer activity of Ag NPs have not been reported. In this report, we showed for the first time that Ag NPs effectively induced cytoprotective autophagy, and that inhibition of autophagy significantly promoted the antitumor activity of Ag NPs, as illustrated in Scheme 1.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of autophagy enhances the anticancer activity of silver nanoparticles

et al. 2014

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Abbreviations: ANXA5, annexin A5; CASP3, caspase 3, apoptosis-related cysteine peptidase; CTSB, cathepsin B; DLS, dynamic light scattering; DMEM, Dulbecco's Modified Eagle's medium; EGFP-LC3, enhanced green fluorescent protein-tagged LC3; ICP-MS, inductively coupled plasma-mass spectrometry; I-MEF, immortalized mouse embryonic fibroblast; MDC, monodansylcadaverine; MTOR, mechanistic target of rapamycin; P-MEF, primary mouse embryonic fibroblast; PI, propidium iodide; PI3K, phosphoinositide 3-kinase; PtdIns3K, phosphatidylinositol 3-kinase; PVP, polyvinylpyrrolidone; RPS6KB, ribosomal protein S6 kinase, 70 kDa; s.c., subcutaneously; SQSTM1, sequestosome 1; TEM, transmission electron microscopy; TUNEL, terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTPdigoxigenin nick end labeling; UV-Vis, ultraviolet visible; XRD, X-ray diffraction.Silver nanoparticles (Ag NPs) are cytotoxic to cancer cells and possess excellent potential as an antitumor agent. A variety of nanoparticles have been shown to induce autophagy, a critical cellular degradation process, and the elevated autophagy in most of these situations promotes cell death. Whether Ag NPs can induce autophagy and how it might affect the anticancer activity of Ag NPs has not been reported. Here we show that Ag NPs induced autophagy in cancer cells by activating the PtdIns3K signaling pathway. The autophagy induced by Ag NPs was characterized by enhanced autophagosome formation, normal cargo degradation, and no disruption of lysosomal function. Consistent with these properties, the autophagy induced by Ag NPs promoted cell survival, as inhibition of autophagy by either chemical inhibitors or ATG5 siRNA enhanced Ag NPs-elicited cancer cell killing. We further demonstrated that wortmannin, a widely used inhibitor of autophagy, significantly enhanced the antitumor effect of Ag NPs in the B16 mouse melanoma cell model. Our results revealed a novel biological activity of Ag NPs in inducing cytoprotective autophagy, and inhibition of autophagy may be a useful strategy for improving the efficacy of Ag NPs in anticancer therapy.

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Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model

Cited by 217 publications

References 35 publications

Cytotoxic Potential and Molecular Pathway Analysis of Silver Nanoparticles in Human Colon Cancer Cells HCT116

Cytotoxic Potential and Molecular Pathway Analysis of Silver Nanoparticles in Human Colon Cancer Cells HCT116

Biosynthesis, Antimicrobial and Cytotoxic Effect of Silver Nanoparticles Using a NovelNocardiopsissp. MBRC-1

Inhibition of autophagy enhances the anticancer activity of silver nanoparticles

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