Antitumor activity of Raddeanin A is mediated by Jun amino‐terminal kinase activation and signal transducer and activator of transcription 3 inhibition in human osteosarcoma

Wang, Zhuoying; Shen, Junhao; Sun, Wei; Zhang, Tao; Zuo, Dongqing; Wang, Hongsheng; Wang, Gangyang; Xu, Jing; Yin, Fei; Mao, Min; Zhou, Zifei; Hua, Yingqi; Cai, Zhengdong

doi:10.1111/cas.14008

Cited by 24 publications

(18 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Osteosarcoma is the commonest non-hematological malignancy of bone in children aged 15–19 years with highly aggressive and systemic metastasis, and characteristically occurs around the growth plate of long bones [17]. In recent decades, a large number of lncRNAs ranging in size from several hundred base pairs to tens of thousands base pairs have been clarified as a novel group of cancer-related non-coding RNAs (ncRNAs) in human.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Degradation of long non-coding RNA-CIR decelerates proliferation, invasion and migration, but promotes apoptosis of osteosarcoma cells

Liu

Kang

et al. 2019

Cancer Cell Int

View full text Add to dashboard Cite

BackgroundOver the years, long non-coding RNAs (lncRNAs) have been clarified in malignancies, this research was focused on the role of lncRNA cartilage injury-related (lncRNA-CIR) in osteosarcoma cells.MethodsLncRNA-CIR expression in osteosarcoma tissues and cells, and adjacent normal tissues and normal osteoblasts was determined, then the relations between lncRNA-CIR expression and the clinicopathological features, and between lncRNA-CIR expression and the prognosis of osteosarcoma patients were analyzed. Moreover, the MG63 and 143B cells were treated with silenced or overexpressed lncRNA-CIR, and then the proliferation, invasion, migration and apoptosis of the cells were evaluated by gain- and loss-of-function approaches. The tumor growth, and proliferation and apoptosis of osteosarcoma cells in vivo were observed by subcutaneous tumorigenesis in nude mice.ResultsWe have found that lncRNA-CIR was up-regulated in osteosarcoma tissues and cells, which was respectively relative to adjacent normal tissues and normal osteoblasts. The expression of lncRNA-CIR was evidently correlated with disease stages, distant metastasis and differentiation of osteosarcoma patients, and the high expression of lncRNA-CIR indicated a poor prognosis. Furthermore, the reduction of lncRNA-CIR could restrict proliferation, invasion and migration, but promote apoptosis of osteosarcoma cells in vitro. Meanwhile, inhibited lncRNA-CIR also restrained tumor growth and osteosarcoma cell proliferation, whereas accelerated apoptosis of osteosarcoma cells in vivo.ConclusionWe have found in this study that the inhibited lncRNA-CIR could decelerate proliferation, invasion and migration, but accelerate apoptosis of osteosarcoma cells, which may provide a novel target for osteosarcoma treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Degradation of long non-coding RNA-CIR decelerates proliferation, invasion and migration, but promotes apoptosis of osteosarcoma cells

Liu

Kang

et al. 2019

Cancer Cell Int

View full text Add to dashboard Cite

show abstract

“…STAT3 is transcription factor that involved in growth and metastasis in several types of cancer cell (22,23). STAT3 is highly expressed in osteosarcoma and may be a key molecular target for developing drugs for human osteosarcoma (24).…”

Section: Discussionmentioning

confidence: 99%

Wogonin Influences Osteosarcoma Stem Cell Stemness Through ROS-dependent Signaling

Koh

Sun

Xing

et al. 2020

In Vivo

View full text Add to dashboard Cite

Backgorund/Aim: Wogonin, a flavonoid-like compound extracted from the root of Scutellaria baicalensis Georgi, has been shown to have anticancer effects against cancer cells. Osteosarcoma is the most malignant type of bone cancer and can appear in any bone, with a high propensity for relapse and metastasis. The present study aimed to assess the anticancer effects of wogonin on osteosarcoma stem cells. Materials and Methods: The cytotoxic effects of wogonin on CD133 + Cal72 osteosarcoma stem cells were assessed through in vitro experiments by MTT assay, transwell assay, sphere-formation assay, flow cytometry, immunocytochemistry and western blotting. Results: Wogonin suppressed stem cell characteristics and the expression of stem cell-related genes by regulating reactive oxygen species (ROS) levels and ROS-related signaling of CD133 + Cal72 cells, effects which were reversed by ROS scavenger N-acetylcysteine. Conclusion: Wogonin may be a promising candidate for successful clinical management of osteosarcoma by regulating ROSrelated mechanisms and stem cell-related genes. Osteosarcoma, the most common type of malignant bone tumor, is most prevalent among children and adolescents and accounts for 2.4% of pediatric cancer cases (1). It is most common among the ages of 10-16 years and the second most common among those aged over 40 years. The tumor is potentially present in bone marrow and then may grow into a mass of tumors and can occur in any bone (2). At present, the first-line treatment for most osteosarcoma involves surgery with intensive adjuvant chemotherapy (3). However, many patients still have poor survival due to relapse and metastasis of osteosarcoma (4). Cancer stem cells (CSCs) are cancer cells that have characteristics related to stem cells, such as self-renewal and differentiation abilities (5). Expression of CSC genes is known to induce cancer metastasis (6). In particular, it was reported that osteosarcoma stem cells have a high capacity for tumor metastasis (2). A pentaspan membrane glycoprotein, CD133, is one of the most well-known biomarkers used for the detection of CSCs. It plays an important role in tumor growth, metastasis, tumorigenesis and tumor relapse (7). Finding a drug candidate involved in down-regulating the expression of genes related to osteosarcoma stem cell behaviour is an urgent need. Reactive oxygen species (ROS) are forms of active oxygen and result from cellular metabolism (8). Some studies have revealed that an increased ROS level reduces the expression of stemness-related genes in CSCs and this effect can be reversed by ROS scavengers such as Nacetylcysteine (NAC) (9, 10). Wogonin (5,7-dihydroxy-8-methoxyflavone) is a flavonoid-like compound that extracted from the root of Scutellaria baicalensis Georgi (Figure 1A) (11). It is wellknown for diverse biological effects such as anti-1077 This article is freely accessible online.

show abstract

“…An in vivo study following exposure of RA in ROS xenograft models also confirmed the anti-cancer effects of RA on human osteosarcoma. Zhuoying and coworkers [124] suggested that RA treatment induced apoptosis and abrogated proliferation in osteosarcoma cells through modulation of the JNK/c-Jun and STAT3 signaling pathways. An in vivo study on tibial xenograft tumor model, administration of apoptosis in OS cells, which lead to the reduction of tumor size.…”

Section: Osteosarcomamentioning

confidence: 99%

Anticancer Potential of Raddeanin A, a Natural Triterpenoid Isolated from Anemone raddeana Regel

et al. 2020

View full text Add to dashboard Cite

Natural compounds extracted from plants have gained immense importance in the fight against cancer cells due to their lesser toxicity and potential therapeutic effects. Raddeanin A (RA), an oleanane type triterpenoid is a major compound isolated from Anemone raddeana Regel. As an anticancer agent, RA induces apoptosis, cell cycle arrest, inhibits invasion, migration and angiogenesis in malignant cell lines as well as in preclinical models. In this systemic review, the pharmacological effects of RA and its underlying molecular mechanisms were carefully analyzed and potential molecular targets have been highlighted. The apoptotic potential of RA can be mediated through the modulation of Bcl-2, Bax, caspase-3, caspase-8, caspase-9, cytochrome c and poly-ADP ribose polymerase (PARP) cleavage. PI3K/Akt signaling pathway serves as the major molecular target affected by RA. Furthermore, RA can block cell proliferation through inhibition of canonical Wnt/β-catenin signaling pathway in colorectal cancer cells. RA can also alter the activation of NF-κB and STAT3 signaling pathways to suppress invasion and metastasis. RA has also exhibited promising anticancer potential against drug resistant cancer cells and can enhance the anticancer effects of several chemotherapeutic agents. Overall, RA may function as a promising compound in combating cancer, although further in-depth study is required under clinical settings to validate its efficacy in cancer patients.

show abstract

Antitumor activity of Raddeanin A is mediated by Jun amino‐terminal kinase activation and signal transducer and activator of transcription 3 inhibition in human osteosarcoma

Cited by 24 publications

References 45 publications

RETRACTED ARTICLE: Degradation of long non-coding RNA-CIR decelerates proliferation, invasion and migration, but promotes apoptosis of osteosarcoma cells

RETRACTED ARTICLE: Degradation of long non-coding RNA-CIR decelerates proliferation, invasion and migration, but promotes apoptosis of osteosarcoma cells

Wogonin Influences Osteosarcoma Stem Cell Stemness Through ROS-dependent Signaling

Anticancer Potential of Raddeanin A, a Natural Triterpenoid Isolated from Anemone raddeana Regel

Contact Info

Product

Resources

About