Antitumor activity of paederosidic acid in human non-small cell lung cancer cells via inducing mitochondria-mediated apoptosis

Yu, Ping; Shi, Lifeng; Song, Meiyan; Yu, Meng

doi:10.1016/j.cbi.2017.02.003

Cited by 11 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are two principal apoptosis pathways: the extrinsic pathway and the intrinsic mitochondrial pathway [ 31 ], which is the key signaling pathway in the induction of apoptosis [ 32 ]. It has been reported that the loss of MMP is a typical phenomenon in mitochondrial-dependent apoptosis [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Lipophilic Grape Seed Proanthocyanidin Exerts Anti-Cervical Cancer Effects in HeLa Cells and a HeLa-Derived Xenograft Zebrafish Model

Zhang

Liu

et al. 2022

Antioxidants

View full text Add to dashboard Cite

Lipophilic grape seed proanthocyanidin (LGSP) synthesized from GSP and lauric acid exhibits an excellent antioxidant and anti-inflammatory effect. However, its anti-cervical cancer activity is still unknown. In this study, the in vitro anti-cervical cancer activity of LGSP on HeLa cell lines was investigated by MTT assay, flow cytometry and Western blot analysis, and its effect was explored by a HeLa-derived xenograft zebrafish model. LGSP exhibited an excellent anti-proliferative effect on HeLa cells by increasing the level of reactive oxygen species, which further induced cell apoptosis and blocked cell cycle progression in the G2/M phase. LGSP-treated HeLa cells showed a reduction in mitochondrial membrane potential, upregulation of the Bax/Bcl-2 ratio, release of cytochrome c into the cytoplasm, and activation of cleaved caspase-9/3 and cleavage of PARP, thus indicating that LGSP induced apoptosis through the intrinsic mitochondrial/caspase-mediated pathway. In the zebrafish model, LGSP effectively suppressed the growth of a HeLa xenograft tumor. These data suggest that LGSP may be a good candidate for the prevention or treatment of cervical cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Lipophilic Grape Seed Proanthocyanidin Exerts Anti-Cervical Cancer Effects in HeLa Cells and a HeLa-Derived Xenograft Zebrafish Model

Zhang

Liu

et al. 2022

Antioxidants

View full text Add to dashboard Cite

show abstract

“…JNK is one of the major subfamilies of MAPK pathway which can activate Bax, cytochrome c and the onset of mitochondrial apoptosis (Yu et al. 2017 ). Akt is a serine–threonine kinase which is critical for regulating cell survival and blocking apoptosis, and acts as a hub in regulating multi-pathways (Gao et al.…”

Section: Discussionmentioning

confidence: 99%

The antitumour effects of eudesmin on lung cancer by inducing apoptosis via mitochondria-mediated pathway in the tumour cells

Sun

Zheng

Yang

2017

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Limonoids possess broad range of biological activities, including antitumour, antimicrobial and antioxidant activities, etc. Eudesmin (EDN) is a type of limonoid which also possesses various activities. However, there is no report on the antitumour lung cancer (LC) activities of this compound.Objective: The present study investigates the antitumour effects of EDN and its potential molecular mechanisms.Materials and methods: The in vitro antitumour effects of EDN on LC A549 cells were evaluated by using MTT assay. The in vivo antitumour effects were investigated on a xenograft athymic nude mouse model. The mice were administered orally with EDN (10, 20 and 40 mg/kg) once daily for 28 days. Effects of EDN on apoptosis-related or signalling proteins (Bcl-2, Bax, caspase-3, caspase-9, P53, Akt and JNK) were assayed by western blot analysis.Results: EDN showed significant inhibitory effects on the growth of LC A549 cells in vitro with the half maximal inhibitory concentration (IC50) of 18.3 μM. By treating with EDN (10, 20 and 40 μM), expression of caspase-3, caspase-9, Bax, P53 and phosphorylated JNK in A549 cells were significantly upregulated, whereas expression of Bcl-2 and Akt phosphorylation were significantly down-regulated. Interestingly, EDN-induced apoptosis could be attenuated by JNK inhibitor. In addition, in vivo experiments also indicated EDN (10, 20 and 40 mg/kg) had significant antitumour effects (p < 0.01) on nude mice.Conclusions:Overall, the results indicated that EDN possesses significant antitumour effects on LC and the possible mechanism might be related to induction of mitochondria-mediated apoptosis.

show abstract

“…The mitochondrial pathway is the key signaling pathway in the induction of apoptosis. 35 The fluorescent microscopy results indicated that 5′-epi-SPA-6952A could collapse MMP in HeLa cells. And the cellular ATP levels were significantly reduced in HeLa cells treated with F I G U R E 1 0 Effect of 5′-epi-SPA-6952A on the expression of apoptosis-related proteins in HeLa cells.…”

Section: Discussionmentioning

confidence: 97%

“…The mitochondrial pathway is the key signaling pathway in the induction of apoptosis . The fluorescent microscopy results indicated that 5′‐ epi ‐SPA‐6952A could collapse MMP in HeLa cells.…”

Section: Discussionmentioning

confidence: 99%

A natural product enhances apoptosis via mitochondria/caspase‐mediated pathway in HeLa cells

Fan

Zhang

Liu

et al. 2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

Cervical cancer is the fourth most lethal human malignancy and the leading cause of death among females around the world. Many antitumor agents have microbial origins. 5′‐epi‐SPA‐6952A is a new 24‐membered macrolide isolated from the cultured broth of Streptomyces diastatochromogenes. Therefore, we studied the activity and molecular mechanism of 5′‐epi‐SPA‐6952A in human cervical carcinoma HeLa cell. The results showed that 5′‐epi‐SPA‐6952A significantly inhibited cell proliferation and migration. In addition, 5′‐epi‐SPA‐6952A obviously increased the production of intracellular reactive oxygen species and DNA damage in HeLa cells. Moreover, nuclear shrinkage of cells, decrease in mitochondrial membrane potential, and upregulation of Bax/Bcl‐2 ratio resulted in the release of cytochrome c, and activation of caspase‐9/3 was observed in HeLa cells treated with 5′‐epi‐SPA‐6952A, which means it enhanced the intrinsic mitochondrial apoptosis. Besides, DNA‐damage associated proteins poly (ADP‐ribose) polymerase (PARP) and p53 were also studied, and the expressions of cleaved‐PARP and p53 were drastically increased in HeLa cells treated with 5′‐epi‐SPA‐6952A. Furthermore, we confirmed that 5′‐epi‐SPA‐6952A affected the survival of HeLa cells by blocking cell cycle progression in the G1 phase. Taken together, the results shows that 5′‐epi‐SPA‐6952A significantly inhibited HeLa cells proliferation via intrinsic mitochondrial apoptosis, cell cycle arrest, and blocking cell migration.

show abstract

Antitumor activity of paederosidic acid in human non-small cell lung cancer cells via inducing mitochondria-mediated apoptosis

Cited by 11 publications

References 18 publications

Lipophilic Grape Seed Proanthocyanidin Exerts Anti-Cervical Cancer Effects in HeLa Cells and a HeLa-Derived Xenograft Zebrafish Model

Lipophilic Grape Seed Proanthocyanidin Exerts Anti-Cervical Cancer Effects in HeLa Cells and a HeLa-Derived Xenograft Zebrafish Model

The antitumour effects of eudesmin on lung cancer by inducing apoptosis via mitochondria-mediated pathway in the tumour cells

A natural product enhances apoptosis via mitochondria/caspase‐mediated pathway in HeLa cells

Contact Info

Product

Resources

About