Antitrypanosomal activity and evaluation of the mechanism of action of diterpenes from aerial parts of Baccharis retusa (Asteraceae)

Ueno, Anderson K.; Barcellos, Aline F.; Costa-Silva, Thaís A.; Mesquita, Juliana Tonini; Ferreira, Daiane Dias; Tempone, André G.; Romoff, Paulete; Antar, Guilherme Medeiros; Lago, João Henrique G.

doi:10.1016/j.fitote.2017.12.016

Cited by 23 publications

(17 citation statements)

References 23 publications

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“…Promastigotes forms were kept in cell culture flasks in M-199 medium supplemented with 10% fetal calf serum (FCS) and 0.25 % hemin at 24 ºC BOD incubator. Macrophages were obtained from the peritoneal cavity of BALB/c mice by washing with RPMI-1640 medium supplemented with 10% FCS and kept at 37ºC in a 5% CO 2 -humidified incubator [22]. Murine fibroblasts NCTC cells (clone L929 ATCC) were kept in cell culture flasks in M-199 medium supplemented with 10% FBS and 20 µg/mL gentamicin at 37ºC in a 5% CO 2 -humidified incubator.…”

Section: Methodsmentioning

confidence: 99%

Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae)

Costa-Silva

Conserva

Galisteo

et al. 2019

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Visceral leishmaniasis is a complex neglected tropical disease caused by Leishmania donovani complex. Its current treatment reveals strong limitations, especially high toxicity. In this context, natural products are important sources of new drug alternatives for VL therapy. Therefore, the antileishmanial and immunomodulatory activity of compounds isolated from Nectandra oppositifolia (Lauraceae) was investigated herein . Methods: The n -hexane extract from twigs of N. oppositifolia were subjected to HPLC/HRESIMS and bioactivity-guided fractionation to afford compounds 1 and 2 which were evaluated in vitro against Leishmania ( L. ) infantum chagasi and NCTC cells. Results: The n -hexane extract displayed activity against L. ( L. ) infantum chagasi and afforded iso linderanolide E ( 1 ) and seco subamolide A ( 2 ), which were effective against L. ( L. ) infantum chagasi promastigotes, with IC 50 values of 57.9 and 24.9 µM, respectively. Compound 2 was effective against amastigotes (IC 50 = 10.5 µM) and displayed moderate mammalian cytotoxicity (CC 50 = 42 µM). The immunomodulatory studies of compound 2 suggested an anti-inflammatory activity, with suppression of IL-6, IL-10, TNF with lack of nitric oxide. Conclusion: This study showed the antileishmanial activity of compounds 1 and 2 isolated from N. oppositifolia . Furthermore, compound 2 demonstrated an antileishmanial activity towards amastigotes associated to an immunomodulatory effect.

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Section: Methodsmentioning

confidence: 99%

Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae)

Costa-Silva

Conserva

Galisteo

et al. 2019

J. Venom. Anim. Toxins incl. Trop. Dis

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“…These compounds were more effective against trypomastigotes, and only ent -16-oxo-17-nor-kauran-19-oic acid was active against the amastigote form. The most active against the trypomastigotes forms was e nt -15β-senecioyl-oxy-kaur-16-en-19-oic acid (IC50 = 3.8 μM; SI = 50 as determined using NCTC cells-clone L929) and its effects were related to interference in the permeability of the plasma membrane of the parasite (probably due its lipophilic characteristics; Ueno et al, 2018).…”

Section: Compounds From Baccharis Genus Are Active Against Trypanosommentioning

confidence: 99%

Asteraceae Plants as Sources of Compounds Against Leishmaniasis and Chagas Disease

et al. 2019

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Leishmaniasis and Chagas disease cause great impact on social and economic aspects of people living in developing countries. The treatments for these diseases are based on the same regimen for over 40 years, thus, there is an urgent need for the development of new drugs. In this scenario, Asteraceae plants (a family widely used in folk medicine worldwide) are emerging as an interesting source for new trypanocidal and leishmanicidal compounds. Herein, we provide a non-exhaustive review about the activity of plant-derived products from Asteraceae with inhibitory action toward Leishmania spp. and T. cruzi . Special attention was given to those studies aiming the isolation (or identification) of the bioactive compounds. Ferulic acid, rosmarinic acid, and ursolic acid ( Baccharis uncinella DC.) were efficient to treat experimental leishmaniasis; while deoxymikanolide ( Mikania micrantha ) and (+)-15-hydroxy-labd-7-en-17-al ( Aristeguietia glutinosa Lam.) showed in vivo anti- T. cruzi action. It is also important to highlight that several plant-derived products (compounds, essential oils) from Artemisia plants have shown high inhibitory potential against Leishmania spp., such as artemisinin and its derivatives. In summary, these compounds may help the development of new effective agents against these neglected diseases.

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“…16,17 At this point, essential oils could be considered as an important source of bioactive compounds due to the antimicrobial, antioxidant, antiviral, and antiparasitic effects. 18 As part of our continuous study to the discovery of antiparasitic natural products, 19 the essential oils from leaves of G. macrophylla from two different populations (São Paulo (SP) and Cubatão (CB) cities) were chemically analyzed and tested in vitro against promastigote forms of L. amazonensis. In order to measure the importance of the identified constituents of essential oils to the antileishmanial activity, a multivariate statistical analysis (MSA) and machine learning approaches were performed.…”

Section: A Comparative Study On Chemical Composition Antileishmanialmentioning

confidence: 99%

A Comparative Study on Chemical Composition, Antileishmanial and Cytotoxic Activities of the Essential Oils from Leaves of Guarea macrophylla (Meliaceae) from Two Different Regions of São Paulo State, Brazil, Using Multivariate Statistical Analysis

Oliveira¹,

Martins²,

Soares³

et al. 2019

J. Braz. Chem. Soc.

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Meliaceae representatives are economically important in several aspects including the production of highly prized woods (mahogany, cedar, etc.), constituents for cosmetics, and insecticides. The present study aimed to verify the chemical composition as well as leishmanicidal and cytotoxic potential of essential oils from leaves of two different populations of Guarea macrophylla collected at cities of São Paulo (population I) and Cubatão (population II), São Paulo State, Brazil. Chemically, the oils showed the predominance of sesquiterpenes: cis-β-guaiene, bicyclogermacrene, viridiflorol, and isolongifolan-7α-ol from population I and α-copaene, E-caryophyllene, cis-β-guaiene, and γ-amorphene from population II. In vitro antileishmanial activity against promastigote forms of Leishmania (L.) amazonensis of essential oils was evaluated and displayed 50% effective concentration (EC 50) values ranging from 11.8 to 20.5 µg mL-1. Furthermore, toxicity against peritoneal macrophages of BALB/c mice was observed, with 50% cytotoxic concentration (CC 50) ranging from 17.7 to > 100 µg mL-1. Multivariate statistical analysis revealed the influence of each constituent of the oils against L. amazonensis being 1,10-di-epi-cubenol, α-amorphene, E-caryophyllene, isopimara-7,15-diene, and β-elemene associated with the antileishmanial potential.

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Antitrypanosomal activity and evaluation of the mechanism of action of diterpenes from aerial parts of Baccharis retusa (Asteraceae)

Cited by 23 publications

References 23 publications

Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae)

Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae)

Asteraceae Plants as Sources of Compounds Against Leishmaniasis and Chagas Disease

A Comparative Study on Chemical Composition, Antileishmanial and Cytotoxic Activities of the Essential Oils from Leaves of Guarea macrophylla (Meliaceae) from Two Different Regions of São Paulo State, Brazil, Using Multivariate Statistical Analysis

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