Antithymocyyte Serum Treatment of Hamstres Inoculated with a Measles Virus Isolated from a Patient with Subacute Sclerosing Panencephalitis

Kibler, Ruthann; Cremer, B. E.

doi:10.3109/08820137309022802

Cited by 10 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As in our previous study (9), ATS-treated newborn hamsters inoculated with Lec virus VOL. 16,1977 on August 5, 2020 by guest http://iai.asm.org/ Downloaded from developed signs of CNS disease, and virus was isolated from the brain.…”

Section: Discussionmentioning

confidence: 70%

“…At the time of onset of overt signs of CNS disease in the ATS-treated hamsters, antibody was absent or present in low titer (9). The serum titer at autopsy of the animal that survived for 1 month with chronic progressive signs of CNS involvement in experiment 2 was only 1:8.…”

Section: Discussionmentioning

confidence: 91%

“…Some studies indicate a reduced cellular response (8,11,12,17,21); others indicate a normal response (13,16,19,20). We (9), among others (6,7), have attempted to develop an animal model to permit study of the immunological factors operative in the disease and in the viral infection. Antithymocyte serum (ATS) treatment of newborn hamsters rendered them susceptible to intraperitoneal (i.p.)…”

Section: Received For Publication 2 November 1976mentioning

confidence: 99%

“…FA technique. The fluorescent-antibody (FA) technique was done as reported previously (9), using the fluorescein-conjugated gamma globulin fraction of pooled sera from hamsters immunized to measles virus HNT, Philadelphia strain 26, adapted to hamsters (5).…”

Section: Received For Publication 2 November 1976mentioning

confidence: 99%

“…Neutralization tests. Neutralizing antibody titers were determined by micro-plaque assay as described previously (9), except for a 6-day rather than a 4-day incubation period prior to the second overlay of agar. The titers are expressed as the reciprocal of the highest dilution of serum causing 80% plaque reduction.…”

Section: Received For Publication 2 November 1976mentioning

confidence: 99%

See 4 more Smart Citations

Complications and immunological studies of measles virus infection in antithymocyte-treated hamsters

Cremer¹,

Hagens²,

Taylor³

et al. 1977

Infect Immun

View full text Add to dashboard Cite

A study was done to investigate possible late central nervous system (CNS) complications of latent measles infection in hamsters. Signs of CNS disease, consisting of myoclonus and paralysis, occurred in some weanling hamsters inoculated intraperitoneally at 3 or 9 days of age with antithymocyte serum (ATS) and measles virus, but no late complications in adult life attributable to measles virus were seen. A single series of ATS injections plus an injection of measles virus resulted in sustained antibody formation postulated due to establishment of a latent viral infection, whereas similar treatment with normal rabbit serum plus virus caused no or minimal antibody response. The majority of hamsters receiving ATS as newborns and ATS plus virus as weanlings also did not produce antibody. This differential effect of ATS may be due to combined elimination of suppressor and helper cells in the latter ATS-treated group and of suppressor cells only in the former ATS-treated group. Cellular immunity could not be evaluated since lymphocytes from inoculated and uninoculated hamsters released equal and variable amounts of 5'Cr for both infected and noninfected labeled, allogeneic hamster target cells.The immunological implications in the pathogenesis of subacute sclerosing panencephalitis (SSPE) induced by latent measles virus infection of the central nervous system (CNS) are not clear at present. Antibody titers to measles virus in serum and cerebrospinal fluid at the time of recognized CNS disease are elevated by all criteria tested. There is less unanimity in reports on cellular immunity (14). Some studies indicate a reduced cellular response (8,11,12,17,21); others indicate a normal response (13,16,19,20). We (9), among others (6, 7), have attempted to develop an animal model to permit study of the immunological factors operative in the disease and in the viral infection. Antithymocyte serum (ATS) treatment of newborn hamsters rendered them susceptible to intraperitoneal (i.p.) injection of an SSPE strain of measles virus (Lec), a route of injection to which the untreated hamster is resistant. Such ATS-treated animals developed delayed overt signs of CNS involvement 3 weeks after virus inoculation. Only a small number ofthe treated hamsters survived, and with time these developed high titers of circulating antibody to measles virus, suggesting development ofa chronic, latent viral infection. Virus was isolated from the ATS-treated hamsters with CNS disease, but not from the survivors tested.

show abstract

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 91%

Section: Received For Publication 2 November 1976mentioning

confidence: 99%

Section: Received For Publication 2 November 1976mentioning

confidence: 99%

Section: Received For Publication 2 November 1976mentioning

confidence: 99%

See 3 more Smart Citations

Complications and immunological studies of measles virus infection in antithymocyte-treated hamsters

Cremer¹,

Hagens²,

Taylor³

et al. 1977

Infect Immun

View full text Add to dashboard Cite

show abstract

Encephalitis after inhalation of measles virus: A pathogenetic study in hamsters

Parhad

Johnson

Wolinsky

et al. 1981

Annals of Neurology

View full text Add to dashboard Cite

A neuroadapted strain of measles virus (HNT) was administered by inhalation to newborn hamsters. Primary replication of virus in the lung was followed by the transient appearance of virus in spleen within 7 to 9 days of inoculation. A terminal encephalitis occurred between 6 and 60 days in 31% of infected hamsters, and virus was recovered by explant culture of these brains. Virus could not be cultured directly from brain or tissue homogenates. At least 7% of hamsters that had survived the infection for two months had antibody to measles virus. The histopathological change in morbid animals was limited to the central nervous system (CNS) and consisted of small foci of necrosis, perivascular mononuclear cell infiltrates, intracytoplasmic and intranuclear inclusions, and vacuolated pyknotic neurons. Immunofluorescent studies disclosed measles antigen in the lungs and brain. This hamster model of measles encephalitis following a "natural" route of inoculation appears to represent a faithful reproduction of certain CNS complications of natural measles infection in humans, i.e., measles encephalitis and subacute sclerosing panencephalitis.

show abstract

Measles Virus-Host Cell Relationships in Subacute Sclerosing Panencephalitis

Fraser¹

1979

Aspects of Slow and Persistent Virus Infections

View full text Add to dashboard Cite

Antithymocyyte Serum Treatment of Hamstres Inoculated with a Measles Virus Isolated from a Patient with Subacute Sclerosing Panencephalitis

Cited by 10 publications

References 23 publications

Complications and immunological studies of measles virus infection in antithymocyte-treated hamsters

Complications and immunological studies of measles virus infection in antithymocyte-treated hamsters

Encephalitis after inhalation of measles virus: A pathogenetic study in hamsters

Measles Virus-Host Cell Relationships in Subacute Sclerosing Panencephalitis

Contact Info

Product

Resources

About