Antithrombotic Peptides from Scolopendra subspinipes mutilans Hydrolysates

Kong, Yi; Li, Shuai; Shao, Yu; He, Zhi Long; Chen, Mei Mei; Xin, Ming; Jiang, Wei

doi:10.1007/s10989-013-9387-3

Cited by 6 publications

(2 citation statements)

References 20 publications

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“…The whole body of SSM has been used for the treatment of blood coagulation-related symptoms such as hemiplegia and is listed in the Korean Herbal Pharmacopeia and Chinese Pharmacopeia. Evidence-based researches on SSM resulted in the discovery of antithrombic peptides and proteins from the venom of SSM 5 6 7 8 . Despite of the several investigations on antithrombic constituents in SSM, there are few studies on the small-molecule secondary metabolites with antithrombotic and antiplatelet activities.…”

Section: Discussionmentioning

confidence: 99%

“…The traditional application of SSM in stroke and stroke-related hemiplegia has attracted attention to discover anticoagulant agents from the centipede. Thus far, peptides and proteins in the venom of SSM have been demonstrated to have an antithrombotic effect 5 6 7 8 . In addition, evidence-based research on SSM resulted in the discovery of a peptide with a potential to be developed into an analgesic as effective as morphine 9 .…”

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confidence: 99%

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Antithrombotic and antiplatelet activities of small-molecule alkaloids from Scolopendra subspinipes mutilans

Lee

Kulkarni

et al. 2016

Sci Rep

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The aim of this study was to discover small-molecule anticoagulants from Scolopendra subspinipes mutilans (SSM). A new acylated polyamine (1) and a new sulfated quinoline alkaloid (2) were isolated from SSM. Treatment with the new alkaloids 1, 2, and indole acetic acid 4 prolonged the activated partial thromboplastin time and prothrombin time and inhibited the activity and production of thrombin and activated factor X. Furthermore, compounds 1, 2, and 4 inhibited thrombin-catalyzed fibrin polymerization and platelet aggregation. In accordance with these potential in vitro antiplatelet activities, compounds 1, 2, and 4 showed enhanced antithrombotic effects in an in vivo pulmonary embolism and arterial thrombosis model. Compounds 1, 2, and 4 also elicited anticoagulant effects in mice. Collectively, this study may serve as the groundwork for commercializing SSM or compounds 1, 2, and 4 as functional food components for the prevention and treatment of pathogenic conditions and serve as new scaffolds for the development of anticoagulants.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Antithrombotic and antiplatelet activities of small-molecule alkaloids from Scolopendra subspinipes mutilans

Lee

Kulkarni

et al. 2016

Sci Rep

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Antithrombotic Activity of Brewers’ Spent Grain Peptides and their Effects on Blood Coagulation Pathways

Cian

Garzón

Martínez‐Augustin

et al. 2018

Plant Foods Hum Nutr

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Antithrombotic activity of brewers' spent grain peptides before and after simulated gastrointestinal digestion and their effects on blood coagulation pathways were evaluated. Two hydrolysates were produced using sequential enzymatic systems: alkaline protease + Flavourzyme (AF) and neutral protease + Flavourzyme (PF). Simulation of gastrointestinal digestion of AF and PF hydrolysates was made using porcine pepsin and pancreatin enzymes, obtaining the corresponding digested samples: AFD and PFD, respectively. Peptides were fractionated by ultrafiltration using a 1 kDa cut-off membrane. Hydrolysates had peptides with medium and low molecular weights (2100 and 500 Da, respectively), and Glu, Asp, Leu, Ala, and Phe were the most abundant amino acids. Gastrointestinal digested hydrolysates presented high proportion of small peptides (~500 Da), and higher amount of Val, Tyr, and Phe than hydrolysates. Mass spectrum (HDMS Q-TOF) of AFD-ultrafiltered fraction <1 kDa exhibited peptides from 500 to 1000 Da, which are not present in AF. PFD showed the generation of new peptides from 430 to 1070 Da. All samples showed thrombin inhibitory activity. However, no effect was observed on prothrombin time. Peptides <1 kDa from hydrolysates and digested samples delayed thrombin and thromboplastin time respect to the control (~63%). Also the samples showed thrombin inhibitory activity at common pathway level. Thus, brewers' spent grain peptides exerted their antithrombotic activity by inhibiting the intrinsic and common pathways of blood coagulation. This is the first report to demonstrate that brewers' spent grain peptides are able to delay clotting time after simulated gastrointestinal digestion.

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Protein Hydrolysates and Biopeptides

Nasri

2017

Advances in Food and Nutrition Research

157

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Antithrombotic Peptides from Scolopendra subspinipes mutilans Hydrolysates

Cited by 6 publications

References 20 publications

Antithrombotic and antiplatelet activities of small-molecule alkaloids from Scolopendra subspinipes mutilans

Antithrombotic and antiplatelet activities of small-molecule alkaloids from Scolopendra subspinipes mutilans

Antithrombotic Activity of Brewers’ Spent Grain Peptides and their Effects on Blood Coagulation Pathways

Protein Hydrolysates and Biopeptides

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