Antithrombotic Effect of FermentedOphiopogon japonicusin Thrombosis-Induced Rat Models

Abstract

In this study, the antithrombotic and thrombolytic ability of second fermented extract of Ophiopogon japonicus (FEOJ) was verified in thrombosis-induced rats. Thrombosis was induced by oral administration of 2% carrageenan for 4 weeks. Five experimental groups (n = 9/group) involved in the study were control group, thrombosis group, low-dose FEOJ group (2 mL/kg, low-dose Ophiopogon japonicus [LOJ]), middle-dose FEOJ group (6 mL/kg, medium-dose Ophiopogon japonicus [MOJ]), and high-dose FEOJ group (12 mL/kg, high-dose Ophiopogon japonicus [HOJ]). The clotting time (CT), bleeding time (BT), prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen (FBG) were assessed in blood samples, and histological studies were performed on liver and lung tissues. The results demonstrated delayed CT only in MOJ and HOJ groups and delayed BT in all FEOJ groups compared with those in thrombosis and control groups (P < .05). Similarly, APTT was significantly delayed only in MOJ and HOJ groups, and PT was significantly delayed in all FEOJ groups, compared with those in control and thrombosis groups (P < .05). Although concentrations of FBG were similar in control, thrombosis, and LOJ groups, the tendency for decreased concentration of FBG (statistically nonsignificant) in MOJ and HOJ groups has been observed. Histological examination of livers and lungs revealed that thrombosis was partially improved in FEOJ group compared with the thrombosis group. In conclusion, CT, BT, PT, and APTT were prolonged in FEOJ group more than in control and thrombosis groups, thereby, depicting antithrombotic and thrombolytic effects. However, concentration-dependent effects of FEOJ were more prominent in MOJ and HOJ groups than in the LOJ group.

Novel steroidal saponins with cytotoxic activities from the roots of Ophiopogon japonicus (L. f.) Ker-Gawl

“…The phytochemical investigation of a 75% EtOH extract of the roots of Ophiopogon japonicus (L. f.) Ker-Gawl resulted in the isolation of seven steroidal saponins (1-7), including six new steroidal saponins (1)(2)(3)(4)(5)(6) and one known steroidal saponin (7). The known compound (7) was identied as (25R)-ruscogenin-1-O-a-L-rhamnopyranosyl-(1 / 2)-[b-D-xylopyranosyl-(1 / 3)]-a-Larabinopyranoside by comparison of its MS, 1 H NMR and 13 C NMR data with published data in the literature.…”

“…2 Pharmacological studies indicated that the tubers of O. japonicus exhibit various biological activities such as immunomodulation, anti-diabetes, cardiovascular protection, anti-oxidation and anti-cancer. [3][4][5][6][7] In recent years, the signicant pharmacological effects of the tubers of O. japonicus on cardiovascular disease and cancer have drawn much attention from medicinal researchers. Previous phytochemical investigations on the tubers of O. japonicas resulted in the isolation of homoisoavonoids, [8][9][10][11][12][13] organic acids, [14][15][16] saccharides 17,18 and steroidal saponins.…”

“…[19][20][21][22][23] However, the bioactive ingredients of this herb are still not fully elucidated. As part of our ongoing progress to search for bioactive constituents from traditional Chinese medicine, a 75% EtOH extract of the roots of O. japonicus was investigated which led to the isolation of six new steroidal saponins (1)(2)(3)(4)(5)(6) and one known steroidal saponin (7) (Fig. 1).…”

Granule of BU-XIN RUAN-MAI Attenuates the Patients’ Angina Pectoris of Coronary Heart Disease via Regulating miR-542-3p/GABARAP Signaling

“…Western medicine has stated that coronary heart disease is a chronic inflammatory process (Yin deficiency with internal heat), lipid accumulation (sputum) and migration and proliferation of smooth muscle cells (blood stasis). Luckily, Granule of BU-XIN RUAN-MAI is just the compound prescription that it may nourish Qi and Yin to enhance blood circulation for clearing internal heat and removing obstruction in collaterals, and products of these herbs exhibit perfect activity of anti-inflammation, antioxidative stress, antithrombotic activity, and cardiovascular protective activity [20][21][22][23][24][25][26][27][28][29][30][31][51][52][53][54][55][56]. Consequently, Granule of BU-XIN RUAN-MAI is a perfect prescription for treatment of ischemic heart disease (angina pectoris).…”

Evaluation Procoagulant Activity and Mechanism of Astragalin

“…However, the side effects limit their use. Natural products with procoagulant activities are current research hot spot [32,33]. In this paper, it was found that astragalin had good coagulant Effects of Astragalin on ESR and PCV (X ± SD, n = 8).…”