Antithrombogenic properties of a nitric oxide-releasing dextran derivative: evaluation of platelet activation and whole blood clotting kinetics

Damodaran, Vinod B.; Leszczak, Victoria; Wold, Kathryn A.; Lantvit, Sarah M.; Popat, Ketul C.; Reynolds, Melissa M.

doi:10.1039/c3ra45521a

Cited by 25 publications

(27 citation statements)

References 62 publications

(88 reference statements)

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“…Incubation was performed at room temperature on a horizontal shaker plate (100 revolutions per minute) for 2 hours with the aim of dispersing coagulation factors produced from activating platelets. These methods are similar to previously used techniques to assess platelet adhesion and activation on various biomaterials 23, 52, 53, 63, 75 . After incubation, samples were fixed with 1% gluteraldehyde and were dried through a series of ethanol treatments.…”

Section: Methodsmentioning

confidence: 95%

Hemodynamic Performance and Thrombogenic Properties of a Superhydrophobic Bileaflet Mechanical Heart Valve

et al. 2016

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In this study, we explore how blood-material interactions and hemodynamics are impacted by rendering a clinical quality 25 mm St. Jude Medical Bileaflet mechanical heart valve (BMHV) superhydrophobic (SH) with the aim of reducing thrombo-embolic complications associated with BMHVs. Basic cell adhesion is evaluated to assess blood-material interactions, while hemodynamic performance is analyzed with and without the SH coating. Results show that a SH coating with a receding contact angle (CA) of 160º strikingly eliminates platelet and leukocyte adhesion to the surface. Alternatively, many platelets attach to and activate on pyrolytic carbon (receding CA=47), the base material for BMHVs. We further show that the performance index increases by 2.5% for coated valve relative to an uncoated valve, with a maximum possible improved performance of 5%. Both valves exhibit instantaneous shear stress below 10 N/m2 and Reynolds Shear Stress below 100 N/m2. Therefore, a SH BMHV has the potential to relax the requirement for antiplatelet and anticoagulant drug regimens typically required for patients receiving MHVs by minimizing blood-material interactions, while having a minimal impact on hemodynamics. We show for the first time that SH-coated surfaces may be a promising direction to minimize thrombotic complications in complex devices such as heart valves.

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Section: Methodsmentioning

confidence: 95%

Hemodynamic Performance and Thrombogenic Properties of a Superhydrophobic Bileaflet Mechanical Heart Valve

et al. 2016

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“…For this reason, endothelium is one of the most thromboresisitive materials. Consequently, materials that can release NO at a steady-state equivalent to that released by the natural endothelium (0.5 × 10 −10 mol cm −2 min −1 ) [ 15 ], can be a suitable material for making biomedical devices with enhanced antifouling capabilities [ 16 , 17 ]. Liu et al recently demonstrated that the presence of NO significantly reduces the formation of Shewanella woodyi ( S. woodyi ) biofilm by simultaneously down-regulating the cyclase activity and up-regulating the phosphodiesterase activity of the diguanylate cyclase gene ( Sw DGC) (Fig.…”

Section: Strategies For Making Antifouling Biomedical Surfacesmentioning

confidence: 99%

“…Additionally, it has been shown that reactive oxygen species, produced by PEG might modulate the cell response [ 46 ]. For these reasons, alternate hydrophilic polymers such as polyglycerols [ 47 ], polyoxazolines [ 48 ], polyamides [ 49 ], and naturally occurring polysaccharides [ 17 ] have been evaluated for antifouling applications.…”

Section: Strategies For Making Antifouling Biomedical Surfacesmentioning

confidence: 99%

Bio-inspired strategies for designing antifouling biomaterials

2016

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Contamination of biomedical devices in a biological medium, biofouling, is a major cause of infection and is entirely avoidable. This mini-review will coherently present the broad range of antifouling strategies, germicidal, preventive and cleaning using one or more of biological, chemical and physical techniques. These techniques will be discussed from the point of view of their ability to inhibit protein adsorption, usually the first step that eventually leads to fouling. Many of these approaches draw their inspiration from nature, such as emulating the nitric oxide production in endothelium, use of peptoids that mimic protein repellant peptides, zwitterionic functionalities found in membrane structures, and catechol functionalities used by mussel to immobilize poly(ethylene glycol) (PEG). More intriguing are the physical modifications, creation of micropatterns on the surface to control the hydration layer, making them either superhydrophobic or superhydrophilic. This has led to technologies that emulate the texture of shark skin, and the superhyprophobicity of self-cleaning textures found in lotus leaves. The mechanism of antifouling in each of these methods is described, and implementation of these ideas is illustrated with examples in a way that could be adapted to prevent infection in medical devices.

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“…Many published methods to investigate the anti-thrombogenic properties of biomaterials focus on studying the early stages of blood clotting, such as protein adsorption, and platelet adhesion and activation. Although they are important to understand the interaction between blood and the implant surface, these studies do not provide significant information about the overall coagulation process (Damodaran et al, 2013;Simon-Walker et al, 2018;Obstals et al, 2018).…”

Section: Thrombogenicitymentioning

confidence: 99%