1992
DOI: 10.1007/bf02399809
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Antithrombin III (AT3) polymorphism in the marsupialMonodelphis domestica: Identification and genetics

Abstract: Antithrombin III polymorphism was observed in the gray short-tailed opossum, Monodelphis domestica, by either one-dimensional polyacrylamide gel electrophoresis (PAGE; pH 7.9), two-dimensional PAGE (agarose, pH 5.4; 12% T, pH 7.9), or isoelectric focusing (pH 4.2-4.9) followed by immunoblotting with rabbit antiserum to human antithrombin III. Family studies demonstrated an inheritance of three codominant autosomal alleles, AT3A, AT3B, and AT3C, and a population study revealed frequencies of 0.70, 0.10, and 0.2… Show more

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Cited by 6 publications
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“…The esterase antiserum was produced by immunizing rabbits with native horse esterase electroeluted from two-dimensional (ZD)-PAGE gels (Patterson 1989). Plasmas (5pl) were also subjected to polyacrylamide isoelectric focusing (PAIEF), 5%T pH4.2-4.9 (Pharmalyte) with 3% N-(2-acetamido)-Z-aminoethane sulphonic acid 408 (ACES; BDH chemicals) (gel dimensions Bell, Arthur, Breen 240xllOx0.5mm) as described by Bell et al (1992) for 3 h at constant 8 W and 15°C and stained for ES activity as described for the I D -PAGE gels.…”
Section: Electrophoresismentioning
confidence: 99%
“…The esterase antiserum was produced by immunizing rabbits with native horse esterase electroeluted from two-dimensional (ZD)-PAGE gels (Patterson 1989). Plasmas (5pl) were also subjected to polyacrylamide isoelectric focusing (PAIEF), 5%T pH4.2-4.9 (Pharmalyte) with 3% N-(2-acetamido)-Z-aminoethane sulphonic acid 408 (ACES; BDH chemicals) (gel dimensions Bell, Arthur, Breen 240xllOx0.5mm) as described by Bell et al (1992) for 3 h at constant 8 W and 15°C and stained for ES activity as described for the I D -PAGE gels.…”
Section: Electrophoresismentioning
confidence: 99%