Antithrombin III (AT3) polymorphism in the marsupialMonodelphis domestica: Identification and genetics

Bell, K.; Arthur, Helen; Oorschot, Roland A.H. van; VandeBerg, John L.

doi:10.1007/pl00020520

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…Proteins were electroblotted from stained (for TF) and unstained (for ES) ID-PAGE gels on to nitrocellulose (0.45 pm) in a Bio-Rad Trans Blot cell at 3 mA/cm2 for 1 2 min and probed with either rabbit antiserum to human TF (Dako) (1:500 dilution) or equine esterase (1:20 dilution) using the procedure described in the Bio-Rad bulletin 1721 (goat anti-rabbit IgG alkaline phosphatase conjugate kit). Plasmas (5pl) were also subjected to polyacrylamide isoelectric focusing (PAIEF), 5%T pH4.2-4.9 (Pharmalyte) with 3% N-(2-acetamido)-Z-aminoethane sulphonic acid 408 (ACES; BDH chemicals) (gel dimensions Bell, Arthur, Breen 240xllOx0.5mm) as described by Bell et al (1992) for 3 h at constant 8 W and 15°C and stained for ES activity as described for the I D -PAGE gels. Plasmas (5pl) were also subjected to polyacrylamide isoelectric focusing (PAIEF), 5%T pH4.2-4.9 (Pharmalyte) with 3% N-(2-acetamido)-Z-aminoethane sulphonic acid 408 (ACES; BDH chemicals) (gel dimensions Bell, Arthur, Breen 240xllOx0.5mm) as described by Bell et al (1992) for 3 h at constant 8 W and 15°C and stained for ES activity as described for the I D -PAGE gels.…”

Section: Electrophoresismentioning

confidence: 99%

Mutations in the equine plasma transferrin and esterase systems

1995

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Eleven apparent mutations of the equine plasma transferrin and esterase gene (10 in TF and one in ES) were found in an analysis of approximately 240 000 thoroughbred horses. Eight of the transferrin mutations produced variants not previously recognized in horses. In the two remaining transferrin mutations and the esterase mutation, reduced plasma concentrations of the proteins were demonstrated by immunological techniques and together with the family data indicated the existence of 'null' alleles.

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Section: Electrophoresismentioning

confidence: 99%

Mutations in the equine plasma transferrin and esterase systems

1995

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Plasma protease inhibitor (PI) system in the laboratory opossum,Monodelphis domestica

et al. 1996

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Protease inhibitor (PI) polymorphism was observed in the laboratory opossum, Monodelphis domestica, by either one-dimensional acid polyacrylamide gel electrophoresis (PAGE; pH 4.6) or isoelectric focusing (pH 3.5-5.0) followed by immunoblotting with rabbit antiserum to human alpha 1-antitrypsin; but acid PAGE produced superior resolution of the PI proteins. Family studies demonstrated an inheritance of nine codominant autosomal alleles, PID, PIE, PIF, PIG, PIH, PII, PIJ, PIK, and PIM, and a population study revealed frequencies of 0.411, 0.010, 0.341, 0.034, 0.023, 0.071, 0.035, 0.020, and 0.055, respectively.

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First-Generation Linkage Map of the Gray, Short-Tailed Opossum,Monodelphis domestica, Reveals Genome-Wide Reduction in Female Recombination Rates

et al. 2004

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The gray, short-tailed opossum, Monodelphis domestica, is the most extensively used, laboratory-bred marsupial resource for basic biologic and biomedical research worldwide. To enhance the research utility of this species, we are building a linkage map, using both anonymous markers and functional gene loci, that will enable the localization of quantitative trait loci (QTL) and provide comparative information regarding the evolution of mammalian and other vertebrate genomes. The current map is composed of 83 loci distributed among eight autosomal linkage groups and the X chromosome. The autosomal linkage groups appear to encompass a very large portion of the genome, yet span a sex-average distance of only 633.0 cM, making this the most compact linkage map known among vertebrates. Most surprising, the male map is much larger than the female map (884.6 cM vs. 443.1 cM), a pattern contrary to that in eutherian mammals and other vertebrates. The finding of genome-wide reduction in female recombination in M. domestica, coupled with recombination data from two other, distantly related marsupial species, suggests that reduced female recombination might be a widespread metatherian attribute. We discuss possible explanations for reduced female recombination in marsupials as a consequence of the metatherian characteristic of determinate paternal X chromosome inactivation. M ETATHERIAN ("marsupial") mammals have a biomedical models, all of which are eutherian species. However, marsupials and eutherians are more closely long history in research as models for organismal related to one another than to any other vertebrate and cellular physiology, endocrinology, and developmodel species (e.g., birds, amphibians, fishes). Thus, mental patterns and processes and more recently have marsupials represent a unique midpoint between euthetaken their place alongside eutherian ("placental") mamrian and nonmammalian vertebrate models. mals as serious subjects for genetically oriented biomediAs a legacy of their common ancestry, marsupials and cal and evolutionary research (reviewed by Samollow eutherians share genetic mechanisms and molecular and Graves 1998; Graves and Westerman 2002). This processes that represent fundamental (ancestral) mamincreased interest in the genetic characteristics of marmalian characteristics. Nevertheless, since their diversupials reflects the growing utility and importance of gence from a common ancestor ‫081-051ف‬ million years comparative models in biomedical research (Womack ago (MYA; Hope et al. 1990;Kumar and Hedges 1998; Pollock et al. 2000; Postlethwait Woodburne et al. 2003) eutherian and marsupial mamet al. 2000) and the broadening recognition of the value mals have evolved many distinctive morphologic, physof the unique phylogenetic position of marsupials in iologic, and genetic variations on these elemental the mammalian lineage. Marsupials are phylogenetimammalian designs. These phylogenetically restricted cally distinct from the more commonly used mammalian differences can be used as c...

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Antithrombin III (AT3) polymorphism in the marsupialMonodelphis domestica: Identification and genetics

Cited by 3 publications

References 23 publications

Mutations in the equine plasma transferrin and esterase systems

Mutations in the equine plasma transferrin and esterase systems

Plasma protease inhibitor (PI) system in the laboratory opossum,Monodelphis domestica

First-Generation Linkage Map of the Gray, Short-Tailed Opossum,Monodelphis domestica, Reveals Genome-Wide Reduction in Female Recombination Rates

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