Antispasmodic effect of Bupi Yichang pill on colonic contraction of rats in vitro

Wang, Yueqi; Wang, Zhengfang; Chang, Xiongfei; Zhang, Shengsheng

doi:10.1016/j.jtcms.2017.07.012

Cited by 1 publication

(1 citation statement)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Acetylcholinesterase has no negative effects on CCh, which is a structural homolog of acetylcholine; when it interacts with the M 2 and M 3 muscarinic receptors, the tone is increased ( 25 ). Acetylcholine induces smooth muscle contraction by acting on voltage-operated Ca 2+ channels (VOCC) and receptor-operated Ca 2+ channels (ROCC), which involve both intracellular calcium storage release and external calcium input ( 26 ). These experimental studies demonstrated that AEJO, when added cumulatively, reduced the maximum tone induced by CCh in a dose-dependent manner, similar to the maximum tone induced by low K + .…”

Section: Discussionmentioning

confidence: 99%

Antispasmodic and antidiarrheal effects of <i>Juniperus oxycedrus</i> L. on the jejunum in rodents

Amrani,

Karim,

Marghich

et al. 2024

J. Smooth Muscle Res.

View full text Add to dashboard Cite

Functional bowel disorders (FBD) have a major potential to degrade the standards of public life. Juniperus oxycedrus L. ( J. oxycedrus ) (Cupressaceae) has been described as a plant used in traditional medicine as an antidiarrheal medication. The present study is the first to obtain information on the antispasmodic and antidiarrheic effects of J. oxycedrus aqueous extract through in vitro and in vivo studies. An aqueous extract of J. oxycedrus (AEJO) was extracted by decoctioning air-dried aerial sections of the plant. Antispasmodic activity was tested in an isolated jejunum segment of rats exposed to cumulative doses of drogue extract. The antidiarrheic activity was tested using diarrhea caused by castor oil, a transit study of the small intestine, and castor oil-induced enteropooling assays in mice. In the jejunum of rats, the AEJO (0.1, 0.3 and 1 mg/ml) diminished the maximum tone induced by low K + (25 mM), while it exhibited a weak inhibitory effect on high K + (75 mM) with an IC 50 =0.49 ± 0.01 mg/ml and IC 50 =2.65 ± 0.16 mg/ml, respectively. In the contractions induced by CCh (10 −6 M), AEJO diminished the maximum tone, similar to that induced by low K + (25 mM). with an IC 50 =0.45 ± 0.02 mg/ml. The inhibitory effect of AEJO on low K + induced contractions was significantly diminished in the presence of glibenclamide (GB) (0.3 µM) and 4-aminopyrimidine (4-AP) (100 µM), with IC 50 values of 1.84 ± 0.09 mg/ml. and 1.63 ± 0.16 mg/ml, respectively). The demonstrated inhibitory effect was similar to that produced by a non-competitive antagonist acting on cholinergic receptors and calcium channels. In castor oil-induced diarrhea in mice, AEJO (100, 200, and 400 mg/kg) caused an extension of the latency time, a reduced defecation frequency, and a decrease in the amount of wet feces compared to the untreated group (distilled water). Moreover, it showed a significant anti-motility effect and reduced the amount of fluid accumulated in the intestinal lumen at all tested doses. These findings support the conventional use of Juniperus oxycedrus L. as a remedy for gastrointestinal diseases.

show abstract

Section: Discussionmentioning

confidence: 99%