Antispasmodic, bronchodilator, vasorelaxant and cardiosuppressant effects of Buxus papillosa

Abstract

BackgroundThe present research was carried out to investigate pharmacological properties of Buxus papillosa C.K. Schneid. (Buxaceae).Methods Buxus papillosa extracts of leaves (BpL), stem (BpS), roots (BpR) and BpL fractions: hexane (BpL-H), aqueous (BpL-A) also plant constituent, cyclomicrobuxine effect were studied in jejunum, atria, aorta and tracheal preparations from rabbit and guine-peg.ResultsCa++ antagonistic effect of BpS, BpR, BpL-H, BpL-A and cyclomicrobuxine were conclusively suggested, when spontaneous contractions of rabbit jejunal preparation was relaxed along with subsequent relaxation of potassium chloride (80 mM) induced contractions. Ca++ antagonistic effect was further confirmed, when a prominent right shift like that of verapamil was observed in Ca++ concentration-response curves, drawn in a tissue pretreated with BpL (0.3–1.0 mg/mL). In rabbit tracheal tissues BpL, BpS, BpR, BpL-H and BpL-A produced a prominent relaxation in contractions induced by potassium chloride (80 mM) and carbachol (1 μm). When tested in rabbit aortic rings, BpL, BpS, BpR, BpL-H and BpL-A showed concentration-dependent (0.1–3.0 mg/mL) vasorelaxant effect against phenylephrine (1 μM) and high K+-induced contractions. In isolated guinea-pig right atria, BpL, BpS, BpR, BpL-H and BpL-A suppressed atrial force of spontaneous contractions, with BpL-A being most potent.ConclusionsOur results reveal that Buxus papillosa possesses gut, airways and cardiovascular inhibitory actions.

In Vivo, Proteomic, and In Silico Investigation of Sapodilla for Therapeutic Potential in Gastrointestinal Disorders

“…Papaverine (a standard phosphodiesterase enzyme (PDE) inhibitor), chloroform, and aqueous extracts of plant caused a dose-dependent inhibition of spontaneous and high K + -induced contractions in isolated rabbit preparations by producing a similar pattern of nonspecific inhibitory response whereas verapamil was found more potent against K + (80 mM) induced contractions. These data proposed that plant extracts produce their relaxant effect through the dual mechanisms with CCB and producing relaxation effect, like PDE enzyme inhibition [18]. Antidiarrheal effect of the Mz fruit extract, mediated possibly through the dual blockade of phosphodiesterase enzyme receptors and Ca 2+ channels, provides evidence for its effectiveness in diarrhea, though additional mechanisms can be ruled out.…”

Genomics approaches to synthesize plant-based biomolecules for therapeutic applications to combat SARS-CoV-2

“… [ 93 ] Bronchodilation compounds Emodin Folium Sennae Anthraquinone Inhibition of acetylcholine mediated contraction of airway smooth muscle [ 71 ] Curcumin Curcuma longa Polyphenol Relaxation of tracheal smooth muscles contraction mediated by KCl. Regulation involves calcium channel blocking and potassium channel opening [ 22 ] Berberine Berberis aristata Alkaloid Inhibition of histamine receptors, cyclooxygenase pathway and nitric oxide which are involved in bronchoconstriction [ 75 ] 9-octadecenamide Anacardium occidentale Amide derivative of fatty acid Inhibition of histamine mediated bronchoconstriction [ 5 ] Cyclomicrobuxine and its derivatives Buxus papillosa Steroidal alkaloids Inhibition of calcium channels involved in bronchoconstriction [ 38 ] Citral Zingiber officinale Terpenoid Bronchodialation by regulation of β-adrenergic receptor [ 57 ] Expectorant compounds chlorogenic acid, 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid Tussilago farfara Caffeoylquinic acids Unknown [ 100 ] Liquiritin apioside, liquiritigenin and liquiritin Glycyrrhiza uralensis Flavonoid Unknown [ 44 ] Caffeoylquinic acids, astersaponins, and aster peptides Aster tataricus Caffeoylquinic acid, saponin and peptide Unknown [ 103 ] Imperialine, imperialine-β-N-oxide, isoverticine, and isoverticine-β-N-oxide Fritillaria wabuensis Alkaloids Unknown ...…”

“…Steroidal alkaloids Inhibition of calcium channels involved in bronchoconstriction [38] Citral Zingiber officinale…”

Phytochemical Composition and Enzyme Inhibition Studies of Buxus papillosa C.K. Schneid

“…This genus is comprised of about 70 different species and is native to China, Europe, America and Asia among other countries. Most of the plants of this genus are distributed in northern America and Eurasia, such as Pakistan, Turkey and China [14][15][16]. This genus is known to contain many important classes of phytochemicals including steroids, alkaloids, terpenoids, among others [17][18][19].…”