Antispasmodic and spasmolytic effects of methanolic extract from seeds of garcinia kola on isolated rat small intestine

Abstract

Summary:The antispasmodic and spasmolytic effects of methanolic extract of seeds of Garcinia kola Heckel were studied on smooth muscle preparations in vitro. The influence of the extract on rat duodenum, jejunum and ileum was investigated using acetylcholine and barium chloride as agonists. The extract exhibited dose-dependent antispasmodic effects on contractions induced by acetylcholine, and dose-dependent spasmolytic effects on spasms induced by cumulatively increased concentrations of acetylcholine and barium chloride. The graded log concentration-response curves for acetylcholine were non-parallel but shifted to the right in the presence of the extract. It is concluded that the Garcinia kola extract inhibits smooth muscle activity via other mechanisms but not involving neither cholinergic nor adrenergic receptor interaction.

Effects of the aqueous extract of Pittosporum mannii Hook. f. (Pittosporaceae) stem barks on spontaneous and spasmogen-induced contractile activity of isolated rat duodenum

“…The most important finding in the present study was that PMAE elicited its relaxant effect only within a very short range of concentrations (10-40 mg/mL) whereas high concentrations (80 mg/mL and above) further induced a slight or a potent transient increase of the tonus (biphasic effect). A similar biphasic effect was observed in several studies with other plant extracts which exhibited a biphasic action on contractility of intestinal smooth muscle cells (Udia et al, 2009;Hu et al, 2010 Data are mean 7SEM of EC 50 , pD 2 and E max in PMAE 5-80 mg/mL (n ¼14-16) treated preparations. Atropine 0.089-1.426 mg/mL (n¼ 7) and promethazine 0.125-2 mg/mL (n¼7) were used as positive controls.…”

Protective effect of methanolic extract of Garcinia indica fruits in 6-OHDA rat model of Parkinson′s disease

“…[51011] Methanolic extract of Garcinia species also reported antioxidant and antispasmodic effects. [1213] Therefore, present study was undertaken to evaluate the effect of methanolic extract of Garcinia indica (GIM) in dopaminergic neuronal loss induced by 6-hydroxydopamine (6-OHDA) in rat model of PD.…”

Modulation in Airway Smooth Muscle Reactivity and Improvement in Lung Function of Cigarette Smokers and Passive Non-smokers Following Garcinia kola treatment

“…13,14 which have been shown to be effective smooth muscle relaxants, 12,18,22 potent antioxidant, [14][15][16]34 antiinflammatory and antigenotoxic agents believed to result from inhibition of cyclo-oxygenase enzyme, a mechanism associated with chemoprevention against carcinogenic substances. 35 We have recently shown a considerable evidence linking kolaviron, a biflavanoid-complex of Garcinia kola seed to vascular smooth muscle relaxation in isolated rabbit aortic arterial rings following precontractions with agonists through receptor operated Ca 2+ channels (ROCCs) and voltage-operated Ca 2+ channels (VOCCs).…”

“…[12][13][14] The pharmacological properties and medicinal values of G. kola Heckel plant are widely reported and linked with various bioactive compounds including Garcinia-biflavonoids GB-1, GB-2 and kolaflavanone with high antioxidant and safety profile. 15 Previous studies have established the hepatoprotective, 16,17 gastroprotective, 18 hypolipidemic, hypoglycaemic 19 and anti-asthmatic 20 effects of G. kola on experimental animal models. It has also become increasingly apparent that G. kola inhibits smooth muscle activity.…”